Androgen receptor 蛋白, Human (His-SUMO)

The androgen receptor protein is a steroid hormone receptor that acts as a ligand-activated transcription factor that regulates gene expression and affects cell proliferation and differentiation. Coactivators and corepressors such as ZBTB7A negatively regulate androgen receptor signaling by recruiting NCOR1 and NCOR2 to androgen response elements on target genes. Androgen receptor Protein, Human (His-SUMO) is the recombinant human-derived Androgen receptor protein, expressed by E. coli , with N-10*His, N-SUMO, C-Myc labeled tag. The total length of Androgen receptor Protein, Human (His-SUMO) is 369 a.a., with molecular weight of ~62.4 kDa.

雄激素受体是一种类固醇激素受体，作为配体激活的转录因子发挥作用，调节真核细胞中的基因表达并影响细胞增殖和分化。其转录活性通过共激活蛋白和辅阻遏蛋白（例如 ZBTB7A）进行微调，它将 NCOR1 和 NCOR2 招募到靶基因上的雄激素反应元件 (ARE)，从而对雄激素受体信号传导和雄激素诱导的细胞增殖发挥负调节作用。此外，转录激活被 NR0B2 抑制。HIPK3 和 ZIPK/DAPK3 在激活但未磷酸化时可以激活雄激素受体。有趣的是，由于缺乏C端配体结合结构域，雄激素受体可能表现出独立于类固醇激素的特定基因组的组成型转录激活，从而增加了其调节功能的复杂性。

Human

E. coli

N-10*His;N-SUMO;C-Myc

P10275 (D551-T919)

367  [NCBI]

DYYFPPQKTCLICGDEASGCHYGALTCGSCKVFFKRAAEGKQKYLCASRNDCTIDKFRRKNCPSCRLRKCYEAGMTLGARKLKKLGNLKLQEEGEASSTTSPTEETTQKLTVSHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALLSSLNELGERQLVHVVKWAKALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRMYSQCVRMRHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKNPTSCSRRFYQLTKLLDSVQPIARELHQFTFDLLIKSHMVSVDFPEMMAEIISVQVPKILSGKVKPIYFHT

Approximately 62.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.