Apolipoprotein E/APOE 蛋白, Mouse (HEK293, Fc)

Apolipoprotein E/APOE is crucial in lipid transport and participates in the production, transformation and clearance of plasma lipoproteins. It interacts with various particles, including chylomicrons and high-density lipoproteins, and binds to cellular receptors such as LDLR and VLDLR, promoting uptake. Apolipoprotein E/APOE Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Apolipoprotein E/APOE protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Apolipoprotein E/APOE Protein, Mouse (HEK293, Fc) is 293 a.a., with molecular weight of 62-66 kDa.

APOE 是一种载脂蛋白，在通过血浆和间质液在器官之间转运脂质方面发挥着至关重要的作用。它是血浆脂蛋白的关键成分，参与其产生、转化和清除。APOE 与各种脂蛋白颗粒相互作用，包括乳糜微粒、乳糜微粒残余物、VLDL 和 IDL，且优先考虑 HDL。此外，它还与一系列细胞受体结合，例如 LDL 受体/LDLR 和 VLDL 受体/VLDLR，促进细胞摄取含有 APOE 的脂蛋白。APOE 还具有肝素结合活性，可与细胞表面的硫酸乙酰肝素蛋白聚糖结合，支持捕获和受体介导的含 APOE 脂蛋白的摄取。值得注意的是，APOE 形成同四聚体，并在 HDL 的生物合成中与 ABCA1 发生功能性相互作用。它还可能与脑脊液中的 APP/A4 淀粉样蛋白-β 肽、MAPT、MAP2 和分泌的 SORL1 以及 PMEL 相互作用，诱导管腔内囊泡上的原纤维成核。

Mouse

HEK293

C-hFc

P08226 (E19-Q311)

11816  [NCBI]

EGEPEVTDQLEWQSNQPWEQALNRFWDYLRWVQTLSDQVQEELQSSQVTQELTALMEDTMTEVKAYKKELEEQLGPVAEETRARLGKEVQAAQARLGADMEDLRNRLGQYRNEVHTMLGQSTEEIRARLSTHLRKMRKRLMRDAEDLQKRLAVYKAGAREGAERGVSAIRERLGPLVEQGRQRTANLGAGAAQPLRDRAQAFGDRIRGRLEEVGNQARDRLEEVREHMEEVRSKMEEQTQQIRLQAEIFQARLKGWFEPIVEDMHRQWANLMEKIQASVATNPIITPVAQENQ

62-66 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution in 20 mM PB, 150 mM NaCl, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.