Fc gamma RIIIA/CD16a 蛋白, Rat (HEK293, His)

Fc γ RIIIA/CD16a is a receptor for the invariant Fc fragment of immunoglobulin γ (IgG). Fc γ RIIIA/CD16a regulates NK cell survival and proliferation and prevents NK cell progenitor cell apoptosis. Fc γ RIIIA/CD16a plays a role in mediating the anti-tumor activity of therapeutic antibodies by triggering TNFA-dependent ADCC, which promotes the entry of the virus into bone marrow cells during secondary infection and subsequent viral replication through antibody-dependent enhancement (ADE). Fc gamma RIIIA/CD16a Protein, Rat (HEK293, His) is the recombinant rat-derived Fc gamma RIIIA/CD16a protein, expressed by HEK293 , with C-His labeled tag. The total length of Fc gamma RIIIA/CD16a Protein, Rat (HEK293, His) is 179 a.a., with molecular weight of 28-33 kDa.

Fc γ RIIIA/CD16a 蛋白是免疫球蛋白 γ (IgG) 不变 Fc 片段的受体，在结合细胞表面显示的聚集抗原-IgG复合物时被最佳激活，启动抗体依赖性细胞毒性 (ADCC)。Fc γ RIIIA/CD16a 介导 IgG 效应作用于自然杀伤 (NK) 细胞，结合感染过程中产生的抗原-IgG 复合物，触发 NK 细胞依赖性细胞因子的产生和脱颗粒。Fc γ RIIIA/CD16a 在产生记忆样适应性 NK 细胞中起着至关重要的作用，调节 NK细胞存活、增殖，阻止 NK 细胞祖细胞凋亡。Fc γ RIIIA/CD16a 在介导治疗性抗体的抗肿瘤活性中发挥作用，在 IgG 包被的肿瘤细胞中触发 TNFA 依赖性 ADCC，并在对聚焦的 IgG 的反应中增强 ADCC。Fc γ RIIIA/CD16a 通过抗体依赖性增强 (ADE) 机制参与发病，促进病毒在继发性感染期间进入骨髓细胞并随后进行病毒复制。

Measured by its binding ability in a functional ELISA.Immobilized rat FCGR3A-His at 10 μg/mL (100 μl/well) can bind biotinylated human IgG1, The EC₅₀ of biotinylated human IgG1 is 59.0-139.0 ng/mL.

Rat

HEK293

C-His

XP_008767961.1 (L22-P200)

304966  [NCBI]

28-33 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.