CD46 蛋白, Human (HEK293, hFc)

CD46 Protein serves as a cofactor for complement factor I, safeguarding cells against complement-induced harm. It aids in sperm-oocyte fusion and acts as a T-cell costimulatory factor, facilitating the formation of T-regulatory 1 cells. These cells secrete interleukin-10, suppressing immune responses and preventing autoimmunity. Pathogens can exploit CD46's immune regulation abilities to induce an immunosuppressive state in T-cells. CD46 Protein, Human (HEK293, hFc) is the recombinant human-derived CD46 protein, expressed by HEK293, with C-hFc labeled tag. The total length of CD46 Protein, Human (HEK293, hFc) is 294 a.a., with molecular weight of ~70.0 kDa.

CD46 蛋白充当补体因子 I 的辅助因子，通过裂解宿主组织上的 C3b 和 C4b 来保护细胞免受补体介导的损伤。它还在受精过程中的精子-卵母细胞融合中发挥作用，并作为 T 细胞的共刺激因子，促进 CD4+ 细胞分化为 T 调节 1 细胞。这些调节性 T 细胞 1 通过分泌白细胞介素 10 来抑制免疫反应，从而预防自身免疫。在微生物感染的情况下，某些病毒和细菌病原体通过与其结合并在 T 细胞中诱导免疫抑制表型来利用 CD46 的免疫调节特性。

Measured by its binding ability in a functional ELISA. Immobilized CD46 at 2 μg/mL can bind Anti-CD46 rabbit monoclonal antibody, the EC₅₀ of human CD46 protein is 0.8-1.38 ng/mL.

Human

HEK293

C-hFc

P15529 (C35-Y328)

4179  [NCBI]

CEEPPTFEAMELIGKPKPYYEIGERVDYKCKKGYFYIPPLATHTICDRNHTWLPVSDDACYRETCPYIRDPLNGQAVPANGTYEFGYQMHFICNEGYYLIGEEILYCELKGSVAIWSGKPPICEKVLCTPPPKIKNGKHTFSEVEVFEYLDAVTYSCDPAPGPDPFSLIGESTIYCGDNSVWSRAAPECKVVKCRFPVVENGKQISGFGKKFYYKATVMFECDKGFYLDGSDTIVCDSNSTWDPPVPKCLKVLPPSSTKPPALSHSVSTSSTTKSPASSASGPRPTYKPPVSNY

Approximately 70.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.