EXO I 蛋白, E. coli (His)

EXO I Protein, documented with high processivity, degrades single-stranded DNA (ssDNA). It also acts as a DNA deoxyribophosphodiesterase, releasing deoxyribose-phosphate moieties post-cleavage at apurinic/apyrimidinic (AP) sites by AP endonuclease or AP lyase. EXO I's dual functionality in ssDNA degradation and deoxyribose-phosphate removal at AP sites underscores its crucial role in DNA repair processes, maintaining genome integrity. EXO I Protein, E. coli (His) is the recombinant E. coli-derived EXO I protein, expressed by E. coli , with N-6*His labeled tag. EXO I Protein, E. coli (His), has molecular weight of ~54.5 kDa.

正如各种研究所记录的那样，EXO I 蛋白作为主题，在单链 DNA (ssDNA) 的降解中表现出高持续性。此外，它还充当 DNA 脱氧核糖磷酸二酯酶，在脱氧核糖磷酸部分的释放中发挥作用。该活性发生在脱嘌呤/脱嘧啶 (AP) 位点 DNA 裂解后，由 AP 核酸内切酶或 AP 裂解酶催化。EXO I 在 ssDNA 降解和 AP 位点脱氧核糖磷酸部分去除中的双重功能反映了其在 DNA 修复过程和维持基因组完整性中的关键作用。

E.coli

E. coli

N-6*His

P04995

946529  [NCBI]

MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNIFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGLPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLVQQYADDKEKVALLKALWQYAEEIV

Approximately 54.5 kDa

Greater than 95% as determined by reducing SDS-PAGE.