FPG 蛋白, E.coli (His)

FPG is a key base excision repair enzyme that recognizes and eliminates damaged bases, especially oxidized purines such as 8-oxoG. It acts on a variety of pathologies, including guanidinohydantoin: C, spiroiminodiahydantoin: C, thymine glycol: G, 5,6-dihydrouracil: G, and 5-hydroxy Uracil: G. FPG Protein, E.coli (His) is the recombinant E. coli-derived FPG protein, expressed by E. coli , with His, tag free. FPG Protein, E.coli (His), has molecular weight of ~30.3 kDa.

FPG 是碱基切除修复中的重要参与者，可解决因氧化或暴露于诱变剂而造成的 DNA 损伤。FPG 作为 DNA 糖基化酶发挥作用，可识别并消除受损碱基，并优先选择氧化嘌呤，例如 7,8-二氢-8-氧代鸟嘌呤 (8-oxoG) 及其衍生物，包括胍基乙内酰脲：C 和螺亚氨基二乙内酰脲：C。此外，FPG 还作用于胸腺嘧啶二醇：G、5,6-二氢尿嘧啶：G 和 5-羟基尿嘧啶：G。值得注意的是，FPG 具有 AP（脱嘌呤/脱嘧啶）裂解酶活性，可在 DNA 链中引入切口。通过消除 β-δ，它会切割 DNA 主链，在被去除的碱基位点产生单链断裂，包含 3'- 和 5'-磷酸。此外，FPG 可有效切割含有 AP 位点的单链 DNA，有助于 DNA 损伤的修复。

E.coli

E. coli

His

P05523

946765  [NCBI]

MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRLSDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKVLRYTDPRRFGAWLWTKELEGHNVLTHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMDNKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLKDFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK

Approximately 30.3 kDa

Greater than 95% as determined by reducing SDS-PAGE.