Kininogen-1 蛋白, Human (409a.a, HEK293, His)

Kininogen-1 is a key regulator in the coagulation process, inhibiting thiol proteases and strategically targeting prekallikrein and factor XI for coagulation regulation. HMW-kininogen prevents thrombin and plasmin-induced platelet aggregation and aids in hemostasis. Kininogen-1 Protein, Human (409a.a, HEK293, His) is the recombinant human-derived Kininogen-1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Kininogen-1 Protein, Human (409a.a, HEK293, His) is 409 a.a., with molecular weight of ~63.0 kDa.

Kininogen-1 是凝血过程中的关键参与者，可作为硫醇蛋白酶的抑制剂。在这种能力下，HMW-激肽原战略性地将前激肽释放酶和因子 XI 定位在因子 XII 附近，这对于凝血调节至关重要。HMW-激肽原积极防止凝血酶和纤溶酶诱导的血小板聚集，有助于止血。相反，LMW-激肽原不参与血液凝固，但对血小板聚集有抑制作用。HMW-激肽原释放的活性肽缓激肽表现出多种生理效应，包括影响平滑肌收缩、诱发低血压、促进尿钠和利尿、降低血糖水平、通过增加血管通透性介导炎症、刺激伤害感受器以及触发释放其他炎症介质如前列腺素。此外，缓激肽具有心脏保护作用，通过内皮源性舒张因子的作用直接或间接发挥作用。kininogen-1 的多方面作用强调了其在协调各种生理过程中的重要性。

Human

HEK293

C-6*His

P01042-2 (Q19-S427)

3827  [NCBI]

QESQSEEIDCNDKDLFKAVDAALKKYNSQNQSNNQFVLYRITEATKTVGSDTFYSFKYEIKEGDCPVQSGKTWQDCEYKDAAKAATGECTATVGKRSSTKFSVATQTCQITPAEGPVVTAQYDCLGCVHPISTQSPDLEPILRHGIQYFNNNTQHSSLFMLNEVKRAQRQVVAGLNFRITYSIVQTNCSKENFLFLTPDCKSLWNGDTGECTDNAYIDIQLRIASFSQNCDIYPGKDFVQPPTKICVGCPRDIPTNSPELEETLTHTITKLNAENNATFYFKIDNVKKARVQVVAGKKYFIDFVARETTCSKESNEELTESCETKKLGQSLDCNAEVYVVPWEKKIYPTVNCQPLGMISLMKRPPGFSPFRSSRIGEIKEETTSHLRSCEYKGRPPKAGAEPASEREVS

Approximately 63.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM HAc-NaAC, 150 mM NaCl, pH 4.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.