LRRC32 蛋白, Human (HEK293, Fc)

LRRC32 is a key regulator of TGF-β activation and maintains TGFB1, TGFB2, and TGFB3 in the latent state during extracellular storage by binding to latency-associated peptide (LAP). LRRC32 competes with LTBP1 for LAP binding and effectively regulates integrin-dependent TGF-β activation. LRRC32 Protein, Human (HEK293, Fc) is the recombinant human-derived LRRC32 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of LRRC32 Protein, Human (HEK293, Fc) is 608 a.a., with molecular weight of 104-110.0 kDa.

LRRC32 是转化生长因子 β（TGFB1、TGFB2 和 TGFB3）的关键调节因子，通过在细胞外储存期间将 TGF-β 维持在潜伏状态，在控制 TGF-β 激活中发挥关键作用。LRRC32 特别与潜伏相关肽 (LAP)（TGF-β 的调节链）相关，对整合素依赖性 TGF-β 激活发挥调节作用。值得注意的是，LRRC32 与 LTBP1 有效竞争 LAP 结合，进一步调节 TGF-β 激活。其重要性延伸至调节激活的调节性 T 细胞 (Treg) 表面的 TGF-β-1 (TGFB1) 激活。此外，LRRC32 的参与对于上腭发育过程中的上皮融合至关重要，它调节 TGF-β-3 (TGFB3) 的激活。LRRC32 与 LAP 的结合可直接与 TGFB1、TGFB2 和 TGFB3 相互作用，调节 TGF-β-1 和 TGF-β-3 的激活，突显其在微调 TGF-β 信号转导中的复杂作用。此外，LRRC32 与 LAPTM4B 相互作用，有助于减少调节性 T 细胞中 TGFB1 的产生。

Human

HEK293

C-hFc

Q14392 (H20-N627)

2615  [NCBI]

HQDKVPCKMVDKKVSCQVLGLLQVPSVLPPDTETLDLSGNQLRSILASPLGFYTALRHLDLSTNEISFLQPGAFQALTHLEHLSLAHNRLAMATALSAGGLGPLPRVTSLDLSGNSLYSGLLERLLGEAPSLHTLSLAENSLTRLTRHTFRDMPALEQLDLHSNVLMDIEDGAFEGLPRLTHLNLSRNSLTCISDFSLQQLRVLDLSCNSIEAFQTASQPQAEFQLTWLDLRENKLLHFPDLAALPRLIYLNLSNNLIRLPTGPPQDSKGIHAPSEGWSALPLSAPSGNASGRPLSQLLNLDLSYNEIELIPDSFLEHLTSLCFLNLSRNCLRTFEARRLGSLPCLMLLDLSHNALETLELGARALGSLRTLLLQGNALRDLPPYTFANLASLQRLNLQGNRVSPCGGPDEPGPSGCVAFSGITSLRSLSLVDNEIELLRAGAFLHTPLTELDLSSNPGLEVATGALGGLEASLEVLALQGNGLMVLQVDLPCFICLKRLNLAENRLSHLPAWTQAVSLEVLDLRNNSFSLLPGSAMGGLETSLRRLYLQGNPLSCCGNGWLAAQLHQGRVDVDATQDLICRFSSQEEVSLSHVRPEDCEKGGLKNIN

104-110.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.