1. 重组蛋白
  2. CD Antigens
  3. NK Cell CD Proteins
  4. CD100/Semaphorin-4D
  5. Semaphorin-4D/SEMA4D 蛋白, Mouse (HEK293, His)

Semaphorin-4D/SEMA4D 蛋白, Mouse (HEK293, His)

目录号: HY-P74563
产品使用指南

Semaphorin-4D/SEMA4D 是 PLXNB1 和 PLXNB2 的细胞表面受体,在细胞间信号传导中发挥着至关重要的作用。它以 PLXNB1 依赖性方式调节 GABA 能突触发育并促进抑制性突触。Semaphorin-4D/SEMA4D 蛋白, Mouse (HEK293, His) 是重组的 Semaphorin-4D/SEMA4D 蛋白,由 HEK293 表达,带有 C-His 标签。Semaphorin-4D/SEMA4D 蛋白, Mouse (HEK293, His) 全长 733 个氨基酸,分子量为 100-110 kDa。

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描述

Semaphorin-4D/SEMA4D is the cell surface receptor for PLXNB1 and PLXNB2 and plays a crucial role in intercellular signaling. It regulates GABAergic synapse development and promotes inhibitory synapses in a PLXNB1-dependent manner. Semaphorin-4D/SEMA4D Protein, Mouse (HEK293, His) is the recombinant mouse-derived Semaphorin-4D/SEMA4D protein, expressed by HEK293 , with C-His labeled tag. The total length of Semaphorin-4D/SEMA4D Protein, Mouse (HEK293, His) is 733 a.a., with molecular weight of 100-110 kDa.

研究背景

Semaphorin-4D (SEMA4D) 作为 PLXNB1 和 PLXNB2 的细胞表面受体,在细胞间信号传导中发挥着至关重要的作用。这种多功能蛋白质涉及多种细胞过程,包括 GABA 能突触发育的调节,它以 PLXNB1 依赖性方式促进抑制性突触的发育。在海马神经元中,SEMA4D 通过激活 PLXNB1 影响发育中神经突的复杂性和树枝化,从而介导 RHOA 激活。此外,SEMA4D 还参与促进小脑颗粒细胞的迁移并诱导 B 细胞聚集,从而增强其体外活力。此外,SEMA4D 通过激活 PTK2B/PYK2、SRC 和磷脂酰肌醇 3-激酶-AKT 途径刺激内皮细胞迁移。作为同二聚体,SEMA4D 与 PLXNB1 和 PLXNB2 相互作用,强调了它在各种细胞和发育过程中的复杂参与。

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

O09126 (M1-R733)

基因 ID

20354  [NCBI]

蛋白结构
N-term
SEMA4D (M1-R733)
Accession # O09126
His
C-term
同用名
SEMA4D; Semaphorin-4D; M-Sema G; Semaphorin-C-like 2; Sema J; CD100; Semacl2
分子量

100-110 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Semaphorin-4D/SEMA4D Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Semaphorin-4D/SEMA4D Protein, Mouse (HEK293, His)
目录号:
HY-P74563
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