Serpin A5 蛋白, Mouse (Baculovirus, His)

Serpin A5 protein is a heparin-dependent serine protease inhibitor that controls proteolytic activity in body fluids. It binds irreversibly to the serine activation site, inactivating serine proteases and regulating intravascular and extravascular proteolysis. Serpin A5 Protein, Mouse (Baculovirus, His) is the recombinant mouse-derived Serpin A5 protein, expressed by Sf9 insect cells , with N-10*His labeled tag. The total length of Serpin A5 Protein, Mouse (Baculovirus, His) is 381 a.a., with molecular weight of ~45.3 kDa.

Serpin A5 蛋白是一种肝素依赖性丝氨酸蛋白酶抑制剂，复杂地控制体液和分泌物中的蛋白水解活性。这种多面蛋白质通过不可逆地结合丝氨酸蛋白酶的丝氨酸激活位点，从而使它们失活来发挥其功能。Serpin A5 在血管内和血管外蛋白水解的调节中发挥作用，在血浆中发挥着至关重要的止血作用。作为促凝血和促炎因子，它抑制活化蛋白 C 因子以及凝血酶/血栓调节蛋白复合物产生活化蛋白 C 因子。同时，它通过抑制凝血酶原、XI 因子、Xa 因子、血浆激肽释放酶和纤溶酶等因子发挥抗凝剂的作用。在精浆中，丝氨酸蛋白酶抑制剂 A5 以生殖系统中的丝氨酸蛋白酶为目标，间接保护男性生殖道成分免受顶体蛋白过度释放。它通过抑制各种酶进一步调节精子活力和受精。在尿液中，该蛋白质抑制纤溶酶原激活剂和激肽释放酶的活性，同时还使参与肿瘤细胞侵袭和转移的膜锚定丝氨酸蛋白酶失活。此外，丝氨酸蛋白酶抑制剂 A5 通过与视黄酸结合，可以作为精浆和尿液中的疏水性激素载体发挥非抑制作用。当糖胺聚糖、肝素、血栓调节蛋白和磷脂囊泡存在时，其抑制效力显着增强。

Mouse

Sf9 insect cells

N-10*His

P70458 (S25-P405)

268591  [NCBI]

SKKKKAKESSVGAVGPPSSKDFAFRLYRALVSESPGQNVFFSPLSVSMSLGMLSLGAGLKTKTQILDGLGLSLQQGQEDKLHKGFQQLLQRFRQPSDGLQLSLGSALFKDPAVHIRDDFLSAMKTLYMSDTFSTNFGNPEIAKKQINNYVAKQTKGKIVDFIKDLDSTHVMIVVNYIFFKAKWQTAFSETNTHKMDFHVTPKRTTQVPMMNREDGYSYYLDQNISCTVVGIPYQGNAIALFILPSEGKMKQVEDGLDERTLRNWLKMFTKRRLDLYLPKFSIEATYKLENVLPKLGIQDVFTTHADLSGITDHTNIKLSEMVHKSMMEVEESGTTAAAITGAIFTFRSARPSSLKIEFTRPFLLTLMEDSHILFVGKVTRP

Approximately 45.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.