2. 重组蛋白
  3. Enzymes & Regulators
  4. Protease Inhibitors
  5. Serpin (Protease Inhibitor)
  6. Serpin B12
  7. Serpin B12 蛋白, Mouse (sf9, His)

Serpin B12 蛋白, Mouse (sf9, His)

目录号: HY-P77197
产品使用指南

Serpin B12 抑制胰蛋白酶和纤溶酶，但不抑制凝血酶、凝血因子 Xa 和尿激酶型纤溶酶原激活剂。此外，它与 SLFN12 相互作用，有助于细胞分化途径，展示了其超越经典蛋白酶抑制的多功能性质。Serpin B12 蛋白, Mouse (sf9, His) 是重组的 Serpin B12 蛋白，由 Sf9 insect cells 表达，带有 C-His 标签。Serpin B12 蛋白, Mouse (sf9, His) 全长 423 个氨基酸，分子量约为 ~58 kDa。

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100 μg   询价  

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描述

Serpin B12 inhibits trypsin and plasmin but not thrombin, coagulation factor Xa, and urokinase-type plasminogen activator. Additionally, it interacts with SLFN12, contributing to cellular differentiation pathways, showcasing its multifunctional nature beyond classical protease inhibition. Serpin B12 Protein, Mouse (sf9, His) is the recombinant mouse-derived Serpin B12 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Serpin B12 Protein, Mouse (sf9, His) is 423 a.a., with molecular weight of ~58 kDa.
研究背景

Serpin B12 是一种多功能蛋白酶抑制剂，对胰蛋白酶和纤溶酶发挥抑制作用，同时不影响凝血酶、凝血因子 Xa 和尿激酶型纤溶酶原激活剂。除了作为蛋白酶调节剂的作用外，Serpin B12 还参与细胞分化，它与 SLFN12 相互作用，有助于形成控制细胞分化复杂过程的途径。这凸显了 Serpin B12 的多功能性质，表明它除了作为蛋白酶抑制剂的经典作用之外，还参与了关键的细胞过程。
种属

Mouse
表达系统

Sf9 insect cells
标签

C-His
蛋白编号

Q9D7P9 (M1-P423)
基因 ID

71869  [NCBI]
蛋白结构
N-term
Serpin B12 (M1-P423)
Accession # Q9D7P9
His
C-term
同用名
SERPINB12; Serpin B12; Serine Protease Inhibitor-clade B12
分子量

Approximately 58 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Serpin B12 Protein, Mouse (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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