TCPTP 蛋白, Mouse (sf9, His)

TCPTP is a non-receptor tyrosine-specific phosphatase that regulates multiple signaling pathways by dephosphorylating proteins such as INSR, EGFR, CSF1R, PDGFR, JAK, Src kinase and STAT. TCPTP acts in the nucleus and cytoplasm to regulate hematopoiesis, inflammation, cell proliferation, differentiation, and glucose homeostasis. TCPTP Protein, Mouse (sf9, His) is the recombinant mouse-derived TCPTP protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of TCPTP Protein, Mouse (sf9, His) is 313 a.a., with molecular weight of ~42 kDa.

TCPTP 是一种非受体型酪氨酸特异性磷酸酶，通过对参与各种信号传导途径的一系列蛋白质进行去磷酸化来发挥其调节作用。这包括受体蛋白酪氨酸激酶，如 INSR、EGFR、CSF1R 和 PDGFR，以及非受体蛋白酪氨酸激酶，如 JAK1、JAK2、JAK3、Src 家族激酶、STAT1、STAT3 和 STAT6。TCPTP 在细胞核和细胞质中发挥作用，对重要的生物过程产生负向调节，包括造血、炎症反应、细胞增殖和分化以及葡萄糖稳态。它在免疫系统发育中发挥着关键作用，影响 T 细胞受体信号传导、巨噬细胞分化和细胞因子介导的途径。此外，TCPTP 参与生长因子信号传导的负调节，其在 EGF 刺激的细胞生长的贴壁依赖性控制中的作用就证明了这一点。TCPTP 被整合素 ITGA1/ITGB1 激活，使 EGFR、PDGFRB 和 SRC 去磷酸化，对其各自的信号通路发挥调节作用。值得注意的是，TCPTP 通过负向调节胰岛素受体信号传导和控制糖异生来扩大其对葡萄糖稳态的影响。此外，它可能表现出 DNA 结合能力。

Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor 988-998 phosphopeptide substrate and the specific activity is > 15 nmoles/min/μg.

Mouse

Sf9 insect cells

N-His

Q06180 (S2-N314)

19255  [NCBI]

Approximately 42 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 20% Glycerol, 0.1 mM TCEP. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.