VLDLR 蛋白, Mouse (HEK293, His)

VLDLR protein is a multifunctional cell surface receptor that plays a crucial role in energy metabolism by binding VLDL and promoting cellular uptake through endocytosis. In addition to lipid transport, VLDLR has broad binding affinities for molecules such as Reelin/RELN, APOE, and CLU. VLDLR Protein, Mouse (HEK293, His) is the recombinant mouse-derived VLDLR protein, expressed by HEK293 , with C-His labeled tag. The total length of VLDLR Protein, Mouse (HEK293, His) is 770 a.a., with molecular weight of 110-130 kDa.

VLDLR 蛋白作为一种在能量代谢中至关重要的多功能细胞表面受体，特别是通过结合 VLDL 并通过内吞作用促进其细胞摄取。除了在脂质转运中的作用外，VLDLR 对各种分子也表现出广泛的结合亲和力，包括 Reelin/RELN、载脂蛋白 E/APOE 含配体和簇蛋白/CLU。在该途径的非活性状态下，VLDLR 与 LRP8 形成同源或异源寡聚体。配体结合后，这些同源寡聚物重排成更高级的受体簇，通过 DAB1 结合在其细胞质尾部，将细胞外 RELN 信号转导至细胞内信号传导过程。这种相互作用会触发 DAB1 磷酸化，协调细胞反应，这对于新生成的神经元的正确定位至关重要。此外，VLDLR 还可作为神经元迁移的停止信号，阻止其进入边缘区。与各种蛋白质（包括 LDLRAP1、SNX17、PCSK9、PAFAH1B3、PAFAH1B2、STX5 和 CLU）的相互作用进一步凸显了 VLDLR 在细胞过程中复杂的调节网络。

Mouse VLDLR, His Tag immobilized on CM5 Chip can bind Mouse PCSK9, His Tag with an affinity constant of 0.28 nM as determined in SPR assay (Biacore T200).

Mouse

HEK293

C-His

P98156 (G28-S797)

22359  [NCBI]

GKKAKCDSSQFQCTNGRCITLLWKCDGDEDCADGSDEKNCVKKTCAESDFVCKNGQCVPNRWQCDGDPDCEDGSDESPEQCHMRTCRINEISCGARSTQCIPVSWRCDGENDCDNGEDEENCGNITCSADEFTCSSGRCVSRNFVCNGQDDCDDGSDELDCAPPTCGAHEFQCSTSSCIPLSWVCDDDADCSDQSDESLEQCGRQPVIHTKCPTSEIQCGSGECIHKKWRCDGDPDCKDGSDEVNCPSRTCRPDQFECEDGSCIHGSRQCNGIRDCVDGSDEVNCKNVNQCLGPGKFKCRSGECIDMSKVCDQEQDCRDWSDEPLKECHINECLVNNGGCSHICKDLVIGYECDCAAGFELIDRKTCGDIDECQNPGICSQICINLKGGYKCECSRGYQMDLATGVCKAVGKEPSLIFTNRRDIRKIGLERKEYIQLVEQLRNTVALDADIAAQKLFWADLSQKAIFSASIDDKVGRHFKMIDNVYNPAAIAVDWVYKTIYWTDAASKTISVATLDGAKRKFLFNSDLREPASIAVDPLSGFVYWSDWGEPAKIEKAGMNGFDRRPLVTEDIQWPNGITLDLVKSRLYWLDSKLHMLSSVDLNGQDRRIVLKSLEFLAHPLALTIFEDRVYWIDGENEAVYGANKFTGSELATLVNNLNDAQDIIVYHELVQPSGKNWCEDDMENGGCEYLCLPAPQINDHSPKYTCSCPNGYNLEENGRECQSTSTPVTYSETKDINTTDILRTSGLVPGGINVTTAVSEVSVPPKGTS

110-130 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.