2. Stem Cell/Wnt Cell Cycle/DNA Damage Epigenetics Anti-infection Autophagy
  3. Organoid Nucleoside Antimetabolite/Analog DNA Methyltransferase Bacterial Autophagy Antibiotic
  4. 5-Azacytidine

5-Azacytidine  (Synonyms: 5-氮杂胞苷; Azacitidine; 5-AzaC; Ladakamycin)

目录号: HY-10586 纯度: 99.56%
COA 产品使用指南

摩尔计算器

5-Azacytidine (Azacitidine; 5-AzaC; Ladakamycin) 是胞苷核苷类似物，特异型抑制 DNA 甲基化。5-Azacytidine 与 DNA 结合共价捕获 DNA甲基转移酶 (DNA methyltransferases)，有益于逆转表观遗传变化。5-Azacytidine 诱导细胞自噬 (autophagy)。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

5-Azacytidine Chemical Structure

5-Azacytidine Chemical Structure

CAS No. : 320-67-2

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥500
In-stock
100 mg ￥346
In-stock
200 mg ￥625
In-stock
500 mg ￥1188
In-stock
1 g   询价  
5 g   询价  

* Please select Quantity before adding items.

Customer Review

5-Azacytidine 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 67 篇科研文献

WB
RT-PCR

    5-Azacytidine purchased from MCE. Usage Cited in: Int J Biol Macromol. 2022.

    5-azacytidine (5-AzaC; 5 μM; 1, 2, 3 days) results in an increase in the expression of MYOD1 and MYOG during myoblast differentiation.

    5-Azacytidine purchased from MCE. Usage Cited in: Cell Death Dis. 2018 Jan 26;9(2):129.  [Abstract]

    The effects of treatment with the indicated epigenetic inhibitors on cleaved PARP (Clv-PARP) expression in both PC9/ER and HCC827/ER cells. β-actin is used as a loading control.

    查看 DNA Methyltransferase 亚型特异性产品:

    查看所有亚型
    DNA Methyltransferase DNMT1 DNMT3 MGMT

    查看 Antibiotic 亚型特异性产品:

    查看所有亚型
    Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    5-Azacytidine (Azacitidine; 5-AzaC; Ladakamycin) is a nucleoside analogue of cytidine that specifically inhibits DNA methylation. 5-Azacytidine is incorporated into DNA to covalently trap DNA methyltransferases and contributes to reverse epigenetic changes[1][2]. 5-Azacytidine induces cell autophagy[4].

    IC50 & Target[1]

    DNMT1

     

    Nucleoside Antimetabolite/Analog

     

    Autophagy

     

    体外研究
    (In Vitro)

    与多种基因相关的未甲基化 CpG 岛在肿瘤中部分或完全甲基化，可被 5-Azacytidine 重新激活[1]。5-Azacytidine 在影响 DNA 甲基转移酶活性的相同浓度范围内作为 Friend 红白血病细胞红细胞分化的弱诱导剂[2]。5-Azacytidine 抑制 L1210 细胞，ID50 和 ID90 值分别为 0.019 和 ~0.15 μg/mL[3]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    5-Azacytidine 相关抗体:
    体内研究
    (In Vivo)

    当动物暴露于 5-Azacytidine (100 mg/kg，腹腔注射) 达 2 小时或更长时间时，TdR-3H 的掺入被显著抑制[3]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    分子量

    244.20

    Formula

    C8H12N4O5
    CAS 号
    性状

    固体

    颜色

    White to yellow

    中文名称

    阿扎胞苷；阿托胞苷；5-氮包苷；5-氮杂胞苷；5-氮杂胞嘧啶核苷；氮胞苷；氮杂胞苷
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    溶解性数据
    In Vitro: 

    DMSO 中的溶解度 : ≥ 31 mg/mL (126.95 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    H2O 中的溶解度 : 25 mg/mL (102.38 mM; 超声助溶 (<60°C))

    * "≥" means soluble, but saturation unknown.

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 4.0950 mL 20.4750 mL 40.9500 mL
    5 mM 0.8190 mL 4.0950 mL 8.1900 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    In Vivo:

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
    ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (8.52 mM); 澄清溶液

      此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

      生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
    • 方案 二

      请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (8.52 mM); 澄清溶液

      此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

      20% SBE-β-CD in Saline 的配制（4°C，储存一周）：2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。

    以下溶解方案，请直接配置工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 20 mg/mL (81.90 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.79%

    COA (198 KB)

    产品使用指南 (1538 KB)
    参考文献
    Kinase Assay
    [3]

    A crude cell-free extract is isolated from LI 210 cells in culture by suspension of the cells in a given volume of 0.05mol/LTris-HCl buffer, pH 7.4, and sonic extraction with a Biosonik at 70% maximal output for 30 sec. The supernatant is collected after centrifugation at 105,000 × g for 60 min (4°C) in a Model L Spinco ultracentrifuge. The final protein concentration of the cell-free extracts is approximately 3 mg/mL. The extracts are used as the source of enzymes. Ribonucleotide reductase activity is measured. A unit of enzyme is defined as the amount that catalyzed dCMP synthesis at a rate of 1 mμmole/hr. The assay systems for the measurement of pyrimidine nucleoside (CR) and deoxynucleoside (TdR, CdR) kinases are essentially those described by Chu and Fischer. However, reactions are terminated by heating for 2 min in a boiling water bath, and the phosphorylated derivatives are isolated according to the method of Bach. Fifty-jul aliquots are applied to 1-inch discs of diethylaminoethyl paper, which are then placed in counting vials and eluted with 0.5 mL of 0.5 mol/LPCA. After 1 hr, 12 mL of Diotol are added, and the radioactivity is determined.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Cell Assay
    [3]

    Twenty mL of cells (circa 1×104 cells/mL) are pipetted into sterilized culture tubes with screw caps and incubated at 37°C overnight. The experiment is initiated by the addition of 1 mL of 5-Azacytidine (5-azaCR) or medium for a given period (from 0 to 240 min) prior to the addition of 1 mL of metabolite (or medium). Cell growth is determined twice a day for 3 days by means of a Model A Coulter counter. To determine IDSO and ID90 values, 5 mL of L1210 cells (5×103 cells/mL) are incubated with the drug at 37°C for 3 days, and cell growth is determined.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [3]

    For the in vivo experiments, leukemic mice (bearing circa 1×103 cells/animal) are given injections i.p. with 0.2 mL of 5-Azacytidine (5-azaCR) of a given concentration. Two hr later, the reaction is started by injecting 0.5 mL of labeled metabolite (TdR-3H or UR-3H, 10 /μCi/12.5 μg). After 1 hr, animals (3 mice/group) are killed by cervical fracture.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    5-Azacytidine 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 4.0950 mL 20.4750 mL 40.9500 mL 102.3751 mL
    5 mM 0.8190 mL 4.0950 mL 8.1900 mL 20.4750 mL
    10 mM 0.4095 mL 2.0475 mL 4.0950 mL 10.2375 mL
    15 mM 0.2730 mL 1.3650 mL 2.7300 mL 6.8250 mL
    20 mM 0.2048 mL 1.0238 mL 2.0475 mL 5.1188 mL
    25 mM 0.1638 mL 0.8190 mL 1.6380 mL 4.0950 mL
    30 mM 0.1365 mL 0.6825 mL 1.3650 mL 3.4125 mL
    40 mM 0.1024 mL 0.5119 mL 1.0238 mL 2.5594 mL
    50 mM 0.0819 mL 0.4095 mL 0.8190 mL 2.0475 mL
    60 mM 0.0683 mL 0.3413 mL 0.6825 mL 1.7063 mL
    80 mM 0.0512 mL 0.2559 mL 0.5119 mL 1.2797 mL
    100 mM 0.0410 mL 0.2048 mL 0.4095 mL 1.0238 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    5-Azacytidine320-67-2Azacitidine 5-AzaC Ladakamycin阿扎胞苷阿托胞苷5-氮包苷5-氮杂胞苷5-氮杂胞嘧啶核苷氮胞苷氮杂胞苷OrganoidNucleoside Antimetabolite/AnalogDNA MethyltransferaseBacterialAutophagyAntibioticDNMTsDNA MTasesInhibitorinhibitorinhibit

    您最近查看的产品:

    Your information is safe with us. * Required Fields.

       产品名称：

    		  

    * 需求量：

    * 客户姓名：

    		 

    * Email：

    * 电话：

    		 

    * 公司或机构名称：

       留言给我们：

    Bulk Inquiry

    Inquiry Information

    产品名称：
    5-Azacytidine
    目录号：
    HY-10586
    需求量：

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

       产品名称：

    		  

    * Lot #：

    * 客户姓名：

    		 

    * Email：

       电话：

    		 

    * 部门：

    * 公司或机构名称：

    		 

       留言给我们：

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.

    嗨！很高兴为您提供帮助！

    尊敬的 MCE 客户您好，
    请您选择所在区域，我们将转接对应客服为您服务！

    热门区域

    A

    B

    C

    F

    G

    H

    J

    L

    N

    Q

    S

    T

    X

    Y

    Z

    热 A B C F G H J L N Q S T X Y Z