2. Apoptosis
  3. Caspase
  4. Ac-DEVD-CHO

Ac-DEVD-CHO 是一种特异性 Caspase-3 抑制剂，Ki 值为 230 pM。

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Custom Peptide Synthesis

Ac-DEVD-CHO Chemical Structure

Ac-DEVD-CHO Chemical Structure

CAS No. : 169332-60-9

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Customer Review

Ac-DEVD-CHO 相关抗体

Top Publications Citing Use of Products

    Ac-DEVD-CHO purchased from MCE. Usage Cited in: Apoptosis. 2019 Oct;24(9-10):703-717.  [Abstract]

    BMDMs were first primed with LPS (500 ng/ml) for 4 h, pre-treated with or without MCC950 (1 μM) and caspase-3 inhibitor Ac-DEVD-CHO (DEVD) for 1 h and followed by stimulation with ATP (5 mM) in the presence or absence of MCC950. The cell lysates are collected and western blotting is performed to assess the expression levels of indicated proteins.

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    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Ac-DEVD-CHO is a specific Caspase-3 inhibitor with a Ki value of 230 pM.

    IC50 & Target[1]

    Caspase 3

    0.23 nM (Ki)

    Caspase-8

    0.92 nM (Ki)

    Caspase-7

    1.6 nM (Ki)

    Caspase-10

    12 nM (Ki)

    Caspase-1

    18 nM (Ki)

    Caspase-6

    31 nM (Ki)

    Caspase-9

    60 nM (Ki)

    Caspase-4

    132 nM (Ki)

    Caspase-5

    205 nM (Ki)

    Caspase-2

    1710 nM (Ki)

    体外研究
    (In Vitro)

    为了确定 caspase-3 在 SLNT 诱导的细胞凋亡中的作用，使用了 caspase-3 抑制剂 (Ac-DEVD-CHO)。添加Ac-DEVD-CHO显著阻止SLNT诱导的细胞凋亡 (从32.91±1.21%下降到15.88±1.58%，而NC和Ac-DEVD-CHO组分别为6.45±0.96%、7.77±0.79%)[2]。
    zVAD-fmk (5.32%) 或Ac-DEVD-CHO (7.43%) 预处理的细胞凋亡率在金丝桃素介导的PDT处理后明显下降[3]。
    值得注意的是，10 μMol/L Ac-DEVD-CHO 部分阻断了 SIN 诱导的细胞凋亡的作用并减少了凋亡细胞核的数量。SIN 的这些作用被 caspase-3 抑制剂 Ac-DEVD-CHO 阻断。阳性对照喜树碱 (4 μM) 可增加 caspase-3 活性，该活性也被 Ac-DEVD-CHO 阻断[4]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Ac-DEVD-CHO 相关抗体:
    体内研究
    (In Vivo)

    与模型组比较，CI 组术后各时间点血清 BUN 浓度均显著降低，Cr 浓度在术后 6 h 显著降低，12 h、24 h 恢复至假手术组；血清 TNF-α、IL-6 浓度降低，IL-10 浓度在所有时间点均显著升高。[TNF-α (ug/L) 6小时：436.2±64.2 vs. 653.6±8.9，12小时：233.4±85.4 vs. 579.7±137.1，24小时：151.0±90.3 vs. 551.0±119.8，IL-6 (ug/L) 6小时：1033.2±345.8 vs. 1 595.3±159.4，12小时：366.3±68.3 vs. 1 330.7±249.8，24小时：241.2±208.4 vs. 815.3±572.7，IL-10 (ug/L) 6小时：33.6±10.4 vs. 26.6±4.5，12小时：37.2±5.0 vs. 24.5±4.3，24小时：38.3±5.5 vs. 18.2±1.6，均P<0.05]；肾细胞凋亡率在各时间点均显著下降：凋亡率6小时：(13.9±3.2) % vs. (18.3±1.4) %，12小时：(10.5±3.6) % vs. (15.9±3.5) %，24小时：(8.4±1.8) % vs. (12.5±2.1) %[5]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    502.47

    Formula

    C20H30N4O11
    CAS 号
    性状

    固体

    颜色

    White to off-white

    Sequence

    N-Acetyl-Asp-Glu-Val-Asp-al
    Sequence Shortening

    Ac-DEVD-al
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    Sealed storage, away from moisture and light, under nitrogen

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

    溶解性数据
    In Vitro: 

    H2O 中的溶解度 : ≥ 50 mg/mL (99.51 mM)

    * "≥" means soluble, but saturation unknown.

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 1.9902 mL 9.9508 mL 19.9017 mL
    5 mM 0.3980 mL 1.9902 mL 3.9803 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    In Vivo:

    以下溶解方案，请直接配置工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 100 mg/mL (199.02 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.94%

    COA (269 KB) RP-HPLC (201 KB) MS (68 KB)

    产品使用指南 (1538 KB)
    参考文献
    Cell Assay
    [4]

    OCLs are incubated with RANKL and treated with 0.5 mM SIN with or without the specific caspase-3 inhibitor Ac-DEVD-CHO (10 μM) for 24 h. At the end of the treatment, the cells are washed with PBS and are stained for 15 min with 10 μM Hoechst 33258 dye. Images of the staineing cells are captured with a fluorescent microscope. The differences are evaluated by counting the number of cells with apoptotic nuclear condensation in each well[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [5]

    One hundred and two male mice are subjected to cecal ligation and puncture or sham operation. The animals are assigned into three equal groups (n=34) according to random number table: sham group, model group, and caspase-3 inhibitor (CI) group. Thirty minutes before CLP, Ac-DEVD-CHO (4 μg/g) is injected subcutaneously in CI group. The levels of blood urea nitrogen (BUN) and creatinine (Cr) are determined, and the concentrations of tumor necrosis factor-α (TNF-α), interleukins (IL-6 and IL-10) are measured by enzyme linked immunosorbent assay (ELISA), the renal cell apoptosis rate is determined by flow cytometry. The 4-day and 7-day survival rates of three groups of mice are observed[5].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    Ac-DEVD-CHO 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 1.9902 mL 9.9508 mL 19.9017 mL 49.7542 mL
    5 mM 0.3980 mL 1.9902 mL 3.9803 mL 9.9508 mL
    10 mM 0.1990 mL 0.9951 mL 1.9902 mL 4.9754 mL
    15 mM 0.1327 mL 0.6634 mL 1.3268 mL 3.3169 mL
    20 mM 0.0995 mL 0.4975 mL 0.9951 mL 2.4877 mL
    25 mM 0.0796 mL 0.3980 mL 0.7961 mL 1.9902 mL
    30 mM 0.0663 mL 0.3317 mL 0.6634 mL 1.6585 mL
    40 mM 0.0498 mL 0.2488 mL 0.4975 mL 1.2439 mL
    50 mM 0.0398 mL 0.1990 mL 0.3980 mL 0.9951 mL
    60 mM 0.0332 mL 0.1658 mL 0.3317 mL 0.8292 mL
    80 mM 0.0249 mL 0.1244 mL 0.2488 mL 0.6219 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Ac-DEVD-CHO169332-60-9CaspaseInhibitorinhibitorinhibit

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