1. Epigenetics PI3K/Akt/mTOR
  2. AMPK
  3. Ampkinone

Ampkinone 是一种间接的 AMP 激活蛋白激酶 (AMPK) 激活剂。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

Ampkinone Chemical Structure

Ampkinone Chemical Structure

CAS No. : 1233082-79-5

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥1779
In-stock
2 mg ¥1000
In-stock
5 mg ¥1600
In-stock
10 mg ¥3000
In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Customer Review

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products
  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Ampkinone is an indirect AMP-activated protein kinase (AMPK) activator.

IC50 & Target[1]

AMPK

 

体外研究
(In Vitro)

Ampkinone stimulates the phosphorylation of AMPK via the indirect activation of AMPK in various cell lines. Ampkinone-mediated activation of AMPK requires the activity of LKB1 and results in increased glucose uptake inmuscle cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Ampkinone-treated DIO mice significantly reduce total body weight and overall fat mass. Histological examination and measurement of lipid parameters show that Ampkinone effectively improves metabolic abnormalities in the DIO mice model[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

505.52

Formula

C31H23NO6

CAS 号
性状

固体

颜色

Light yellow to yellow

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
溶解性数据
In Vitro: 

DMSO 中的溶解度 : 50 mg/mL (98.91 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.9782 mL 9.8908 mL 19.7816 mL
5 mM 0.3956 mL 1.9782 mL 3.9563 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 2 years; -20°C, 1 year。-80°C储存时,请在2年内使用, -20°C储存时,请在1年内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

In Vivo:

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (4.95 mM); 悬浊液; 超声助溶

    此方案可获得 2.5 mg/mL的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;再向上述体系中加入 50 μL Tween-80,混合均匀;然后再继续加入 450 μL 生理盐水 定容至 1 mL

    生理盐水的配制:将 0.9 g 氯化钠,溶解于 ddH₂O 并定容至 100 mL,可以得到澄清透明的生理盐水溶液。
动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
请输入您的动物体内配方组成:
%
DMSO +
+
%
Tween-80 +
%
Saline
如果您的动物是免疫缺陷鼠或者体弱鼠,建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
方案所需 助溶剂 包括:DMSO ,均可在 MCE 网站选购。 Tween 80,均可在 MCE 网站选购。
计算结果
工作液所需浓度 : mg/mL
储备液配制方法 : mg 药物溶于 μL  DMSO(母液浓度为 mg/mL)。
您所需的储备液浓度超过该产品的实测溶解度,以下方案仅供参考,如有需要,请与 MCE 中国技术支持联系。
动物实验体内工作液的配制方法 : 取 μL DMSO 储备液,加入 μL  μL ,混合均匀至澄清,再加 μL Tween 80,混合均匀至澄清,再加 μL 生理盐水
连续给药周期超过半月以上,请谨慎选择该方案。
请确保第一步储备液溶解至澄清状态,从左到右依次添加助溶剂。您可采用超声加热 (超声清洗仪,建议频次 20-40 kHz),涡旋吹打等方式辅助溶解。
纯度 & 产品资料

纯度: 99.28%

参考文献
Kinase Assay
[1]

Total AMPK activity is measured using a synthetic SAMS peptide substrate and [γ-32P]ATP. Briefly, 500 μg of protein extract is incubated with anti-AMPK-α1 and α2 antibodies for 2 h at 4°C. Protein A/G sepharose and agarose are added, and the mixtures are incubated for 3 h at 4°C. After the samples are washed three times with RIPA buffer, the activity is assessed in AMPK reaction buffer containing 20 mM HEPES-NaOH (pH 7.0), 0.4 mM Dithiothreitol, and 0.01% Brij-35, with or without 300 μM AMP. The immune complexes are added to 23 μL of reaction buffer per assay, and then 7 μL of SAMS substrate peptide (HMRSAMSGLHLVKRR, 100 μM final concentration) and 10 μL of ATP mixture (an aliquot of 1 μCi/μL: 90 μL of 75 mM magnesium chloride, 500 μM unlabeled ATP in 20 mM MOPS, pH7.2, 25 mMβ-glycerophosphate, 5 mM EGTA, 1 mM sodium orthovanadate, and 1 mM Dithiothreitol) are added, and the mixture is incubated at 30°C for 15 min. After 35 μL is spotted onto the center of P81 paper, the paper is washed three times with 0.75% phosphoric acid and once with acetone for 5 min. The samples are read using a scintillation counter[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Mice[1]
C57BL/6J mice are used and housed individually in a room maintained at 25°C on a 12/12 h light/dark schedule. For dietinduced obesity (DIO) mice, 4-week-old male C57BL/6J mice are fed a high-fat diet (HFD, 60% calories from fat) ad libitum for 8 weeks. Ampkinone in polyethylene glycol (PEG400) or vehicle is administered subcutaneously at 10 mg/kg body weight per day for 1 month. Body weight and food intake are measured every 3 days. All animals are given an insulin tolerance test (ITT) and sacrificed at 30 days. Liver weight and fat masses are measured, sectioned, and stained with Oil-red O and H&E.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

Ampkinone 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 2 years; -20°C, 1 year。-80°C储存时,请在2年内使用, -20°C储存时,请在1年内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9782 mL 9.8908 mL 19.7816 mL 49.4540 mL
5 mM 0.3956 mL 1.9782 mL 3.9563 mL 9.8908 mL
10 mM 0.1978 mL 0.9891 mL 1.9782 mL 4.9454 mL
15 mM 0.1319 mL 0.6594 mL 1.3188 mL 3.2969 mL
20 mM 0.0989 mL 0.4945 mL 0.9891 mL 2.4727 mL
25 mM 0.0791 mL 0.3956 mL 0.7913 mL 1.9782 mL
30 mM 0.0659 mL 0.3297 mL 0.6594 mL 1.6485 mL
40 mM 0.0495 mL 0.2473 mL 0.4945 mL 1.2364 mL
50 mM 0.0396 mL 0.1978 mL 0.3956 mL 0.9891 mL
60 mM 0.0330 mL 0.1648 mL 0.3297 mL 0.8242 mL
80 mM 0.0247 mL 0.1236 mL 0.2473 mL 0.6182 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
Ampkinone
目录号:
HY-12831
需求量: