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  3. Fluorescent Dye
  4. Bromothymol Blue

Bromothymol Blue  (Synonyms: 溴百里酚蓝)

目录号: HY-D0012 纯度: ≥98.0%
COA 产品使用指南

摩尔计算器

Bromothymol Blue 是一种 pH 指示剂，是测定弱酸弱碱时使用的一种指示剂，其酸性时为黄色，碱性时为蓝色。

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Bromothymol Blue Chemical Structure

Bromothymol Blue Chemical Structure

CAS No. : 76-59-5

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1 g ￥330
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Customer Review

Bromothymol Blue 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Bromothymol Blue is a pH indicator.

体外研究
(In Vitro)

A first characterization and comparison is done by developing an easy and direct measurement method based on a pH indicator system using Bromothymol Blue (BTB) as the indicator and potassium phosphate as the buffer. A pH-shift assay is developed on the basis of Bromothymol Blue as the pH indicator and potassium phosphate as the buffer component[1]. Three pH indicators are tested for the direct determination of 2- 2-keto-L-gulonic acid (2-KLG) production on a plate. The results show that Bromothymol Blue is superior to the other two indicators in terms of the obvious color change and a suitable pH range (blue to yellow at pH 6.5-7.5). Upon the addition of a Bromothymol Blue solution (0.1%, w/v) to an agar plate, zones surrounding colonies of K. vulgare 07 mutants change their color from blue to yellow because K. vulgare 07 mutants release 2-KLG on agar plates, thereby acidifying surrounding areas around colonies[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Bromothymol Blue 相关抗体:
分子量

624.38

Formula

C27H28Br2O5S
CAS 号
性状

固体

颜色

Pale purple to purple

中文名称

溴麝香草酚蓝；溴百里酚蓝；溴百里香酚蓝；溴百里香酚蓝；溴化麝香草酚蓝；溴瑞香草酚蓝
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO 中的溶解度 : 100 mg/mL (160.16 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

H2O 中的溶解度 : < 0.1 mg/mL (insoluble)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.6016 mL 8.0079 mL 16.0159 mL
5 mM 0.3203 mL 1.6016 mL 3.2032 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献
Kinase Assay
[1]

For all three enzymes, an expression in the 96-deep well scale is performed. Therefore, electrocompetent E. coli ArcticExpress (DE3) cells are transformed with the corresponding plasmid. Single clones are picked using the CP7200 Colony Picker and transferred to 96-deep well plates filled with 1.2 mL autoinduction media by a MicroFlo Select dispenser. After incubation (36 h, 37°C at 1,000 rpm), further processing is done manually. First, 100 μL of cell culture is transferred into a 96-well plate (U-shaped bottom) and harvested by centrifugation (4,570 rpm, 10 min at RT) while the supernatant is discarded. The frozen pellets (1 h at -20°C) are thawed at room temperature for one hour to improve cell lysis. Lysis is continued by the addition of 30 μL lysis buffer (3 h, 1,000 rpm, 37°C) containing 2 mM KPi, pH 7.0, 2 mM MgCl2, 10 μg/mL DNaseI, 100 μg/mL lysozyme. Next, 120 μL buffer (2 mM KPi, pH 7.0) is added followed by centrifugation (3,000 rpm, 15 min at RT). For the photometric measurement, 20 μL of the crude extract is transferred to a 96-well plate (F-shaped bottom) and the reaction is started by adding 180 μL master mix to give a final volume of 200 μL (2.5 mM KPi, pH 7.0, 2 mM MgCl2, 25 μg/mL Bromothymol Blue (BTB) and 5 mM keto-deoxy-D-glucarate). The measurements are carried out for 60 min at 2-min intervals. Depending on the enzyme, different time windows are used for the activity calculation[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

Bromothymol Blue 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6016 mL 8.0079 mL 16.0159 mL 40.0397 mL
5 mM 0.3203 mL 1.6016 mL 3.2032 mL 8.0079 mL
10 mM 0.1602 mL 0.8008 mL 1.6016 mL 4.0040 mL
15 mM 0.1068 mL 0.5339 mL 1.0677 mL 2.6693 mL
20 mM 0.0801 mL 0.4004 mL 0.8008 mL 2.0020 mL
25 mM 0.0641 mL 0.3203 mL 0.6406 mL 1.6016 mL
30 mM 0.0534 mL 0.2669 mL 0.5339 mL 1.3347 mL
40 mM 0.0400 mL 0.2002 mL 0.4004 mL 1.0010 mL
50 mM 0.0320 mL 0.1602 mL 0.3203 mL 0.8008 mL
60 mM 0.0267 mL 0.1335 mL 0.2669 mL 0.6673 mL
80 mM 0.0200 mL 0.1001 mL 0.2002 mL 0.5005 mL
100 mM 0.0160 mL 0.0801 mL 0.1602 mL 0.4004 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bromothymol Blue76-59-5溴麝香草酚蓝溴百里酚蓝溴百里香酚蓝溴化麝香草酚蓝溴瑞香草酚蓝Fluorescent DyeInhibitorinhibitorinhibit

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