1. Dye Reagents

CFSE (Synonyms: 5(6)-Carboxyfluorescein diacetate succinimidyl ester; CFDA-SE; 5(6)-CFDA N-succinmidyl ester)

目录号: HY-D0938
产品使用指南

CFSE 是可以跟踪细胞分裂的荧光染料。

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CFSE Chemical Structure

CFSE Chemical Structure

CAS No. : 150347-59-4

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Description

Carboxyfluorescein diacetate succinimidyl ester (CFSE) is a fluorescent dye which can track the cell division.

In Vitro

Carboxyfluorescein diacetate succinimidyl ester (CFSE) is a fluorescent dye which can track the cell division[1].To examine the ability of immune cells to migrate into live slices, a single-cell suspension of autologous splenocytes or PBMC is isolated, label them with CFSE, and add them to the top of the slices on day 2 of culture. After 6 additional days of culture, the CFSE-labeled immune cells (green) are found to have migrated throughout the slices. No positive cells are found in the slices without the addition of CFSE-labeled immune cells. The presence of adoptively transfer leukocytes in the slice cultures 6 d after addition to the culture system demonstrates the feasibility of using slice cultures as a preclinical model for the evaluation of adoptive cellular immunotherapies[2].

References
Preparing Stock Solutions
Concentration Volume (DMSO) Mass 1 mg 5 mg 10 mg
1 mM 1.7939 mL 8.9693 mL 17.9385 mL
5 mM 0.3588 mL 1.7939 mL 3.5877 mL
10 mM 0.1794 mL 0.8969 mL 1.7939 mL
Cell Assay

Single-cell suspensions of splenocytes (or PBMC) are stained with 1 µM CFSE in PBS for 9 min at 37°C, combine with 20 mL of 10% FBS RPMI-1640 medium at RT for 2 min, centrifuge, wash, and count. 2×106 CFSE-labeled splenocytes are added to the top of the slices in 24-well membrane culture insert. After 6 d co-culture, slices are fixed with 4% paraformaldehyde and stained the nuclei with DAPI. Finally, slices are visualized with the confocal microscope with Z stack of 2 µM/step. The image process is analyzed by software[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

557.46

Formula

C₂₉H₁₉NO₁₁

CAS No.

150347-59-4

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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产品名称:
CFSE
目录号:
HY-D0938
需求量: