1. Dye Reagents

CFSE (Synonyms: 5(6)-Carboxyfluorescein diacetate succinimidyl ester; CFDA-SE; 5(6)-CFDA N-succinmidyl ester)

目录号: HY-D0938 纯度: 90.91%
Data Sheet SDS 产品使用指南

CFSE 是可以跟踪细胞分裂的荧光染料。

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CFSE Chemical Structure

CFSE Chemical Structure

CAS No. : 150347-59-4

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Size Price Stock Quantity
10 mM * 1 mL in DMSO ¥1594 In-stock
5 mg ¥1300 In-stock
10 mg ¥2100 In-stock
25 mg ¥4200 In-stock
50 mg ¥7600 In-stock
100 mg   Get quote  
200 mg   Get quote  

* Please select Quantity before adding items.

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Description

CFSE is a fluorescent dye which can track the cell division.

In Vitro

CFSE is a fluorescent dye which can track the cell division[1]. To examine the ability of immune cells to migrate into live slices, a single-cell suspension of autologous splenocytes or PBMC is isolated, labeling them with CFSE, and adding them to the top of the slices on day 2 of culture. After 6 additional days of culture, the CFSE-labeling immune cells (green) are found to have migrated throughout the slices. No positive cells are found in the slices without the addition of CFSE-labeling immune cells[2].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 1.7939 mL 8.9693 mL 17.9385 mL
5 mM 0.3588 mL 1.7939 mL 3.5877 mL
10 mM 0.1794 mL 0.8969 mL 1.7939 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay
[2]

Single-cell suspensions of splenocytes (or PBMC) are stained with 1 µM CFSE in PBS for 9 min at 37°C, combined with 20 mL of 10% FBS RPMI-1640 medium at RT for 2 min, centrifuged, washed, and counted. 2×106 CFSE-labeling splenocytes are added to the top of the slices in 24-well membrane culture insert[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

557.46

Formula

C₂₉H₁₉NO₁₁

CAS No.

150347-59-4

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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产品名称:
CFSE
目录号:
HY-D0938
需求量: