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Calcein-AM  (Synonyms: 钙黄绿素-AM; Calcein acetoxymethyl ester)

目录号: HY-D0041 纯度: 97.34%
COA 产品使用指南

Calcein AM,中文名为该黄绿素乙酰氧基甲指,具细胞膜渗透性,可轻易进入到细胞内,Calcein AM 身并无荧光,进入细胞后被细胞中内源性酯酶水解生成具有强负电荷的不能通透细胞膜的极性分子钙黄绿素 (Calcein),从而被滞留在细胞内。而 Calcein 可发出强绿色荧光,因此常与 Propidium Iodide 用于细胞活力/毒力检测,激发/发射波长:494/515 nm。

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Calcein-AM Chemical Structure

Calcein-AM Chemical Structure

CAS No. : 148504-34-1

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规格 价格 是否有货 数量
100 μg(2 mg/mL * 50 μL in DMSO) ¥1400
In-stock
500 μg(2 mg/mL * 250 μL in DMSO) ¥2500
In-stock

* Please select Quantity before adding items.

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Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 36 篇科研文献

IF

    Calcein-AM purchased from MCE. Usage Cited in: Bioact Mater. 2022 Aug 11;21:20-31.  [Abstract]

    HUVECs proliferation is assessed by Calcein-AM, and the results show that HP-β-CD and HP-β-CD@Res could effectively promote the proliferation of HUVECs.

    Calcein-AM purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2022 Oct;9(30):e2203031.  [Abstract]

    XPS analysis of CaALN powder before and after immersing in FeCl3 solution for 24 h. Detection of intracellular Fe concentration using Calcein-AM in MGC 803 cells after treatment with NaALN, CaALN, and DFX at 25 mg/L for 24 h by FCM analysis. Then these cells are washed with PBS and incubated with Calcein-AM (0.5 μM) for 15 min at 37 °C in the dark.

    Calcein-AM purchased from MCE. Usage Cited in: Pharmaceuticals. 2022 Aug 26;15(9):1059.

    For U251 cells, Calcein-AM (10 μM) solution and 0.5 μL PI staining solution (15 μM) is added into each well, stained for 10 min.

    Calcein-AM purchased from MCE. Usage Cited in: Antioxidants (Basel). 2021 May 18;10(5):798.  [Abstract]

    HeLa cells are pretreated with 20 μM Fe(II) for 2 h and then incubated with Calcein-AM ( 0.5 μM; for 15 min at 37 °C). After 15 min, cells are washed and incubated with or without CAPE and its analogues for 15 min. The fluorescence intensity of cells is measured by flow cytometry.

    Calcein-AM purchased from MCE. Usage Cited in: Biochem Pharmacol. 2021 Jun;188:114583.  [Abstract]

    Cells are incubated with fluorescence probes Calcein-AM (1 μM) and propidium iodide (5 μM) for 5 min and imaged through fluorescence inverted microscope.

    Calcein-AM purchased from MCE. Usage Cited in: Acta Pharm Sin B. 2020 Sep;10(9):1694-1708.  [Abstract]

    HUVECs are pre-treated with SMI or Rd for 24 h and seeded on Matrigel for 5.5 h followed by 0.5 h of Calcein-AM staining.

    Calcein-AM purchased from MCE. Usage Cited in: EMBO Rep. 2019 Jul;20(7):e47563.  [Abstract]

    Cells are trypsinized, washed twice with 0.5 ml PBS, and then incubated with 0.2 μM Calcein‐AM at 37°C for 15 min. Then, the cells were washed twice with 0.5 ml PBS and either incubated with 100 μM Deferiprone or left untreated at 37°C for 1 h. The cells are analyzed with a flow cytometer. Calcein-AM is excited at 488 nm, and fluorescence is measured at 525 nm.
    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Calcein AM, has cell membrane permeability and can easily enter the cell. Calcein AM has no fluorescence and is hydrolyzed by endogenous esterase in the cell to produce polar molecule Calcein (Calcein), which has strong negative charge and cannot permeate the cell membrane. Calcein can emit strong green fluorescence, so it is often used with Propidium Iodide for cell viability/virulence detection, excitation/emission wavelength: 494/515 nm[1].

    体外研究
    (In Vitro)

    使用方法
    1.Calcein AM 工作液的配制
    用 DMSO 配置 5 mM 储备液,再用预热好的无血清细胞培养基或 PBS 稀释储存液,配制成 1-10 μM 的 Calcein AM 工作液。
    注:请根据实际情况调整 Calcein AM 工作液浓度,且现用现配。
    2. 细胞染色(悬浮细胞)
    2.1 离心收集细胞,加入 PBS 洗涤两次,每次 5 分钟。细胞密度在 1×106/mL。
    2.2 加入 1 mL Calcein AM 工作液,37℃ 孵育 30-45 分钟。
    2.3 400 g,离心 3-4 分钟,弃去上清。
    2.4 加入 PBS 洗涤细胞两次,每次 5 分钟。
    2.5 用 1 mL 无血清培养基或 PBS 重悬细胞后,使用荧光显微镜或流式细胞仪进行观察。
    3. 细胞染色(贴壁细胞)
    3.1 将贴壁细胞培养于无菌盖玻片上。
    3.2 从培养基中移出盖玻片,吸除多余培养基。
    3.3 加入 100 μL 染料工作液,轻轻晃动使其完全覆盖细胞,37℃ 孵育 30-45 分钟。
    3.4 吸去染料工作液,用培养基洗 2-3 次,每次 5 分钟,使用荧光显微镜或荧光酶标检测仪进行观察。

    保存条件
    -20℃ 避光保存

    注意事项
    1. 请根据实际情况调整 Calcein AM 工作液浓度与孵育时间。
    2. 本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品。
    3. 为了您的安全和健康,请穿实验服并戴一次性手套操作。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    体内研究
    (In Vivo)

    Calcein-AM 被发现适用于体内研究,因为它对细胞功能没有有害影响,而且确实是细胞活力的标志物[2]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    分子量

    994.86

    Formula

    C46H46N2O23

    CAS 号
    性状

    固体

    颜色

    White to off-white

    Emission (Em)

    515

    Excitation (Ex)

    485

    中文名称

    钙黄绿素-AM

    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式

    -20°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    溶解性数据
    In Vitro: 

    DMSO 中的溶解度 : 100 mg/mL (100.52 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 1.0052 mL 5.0258 mL 10.0517 mL
    5 mM 0.2010 mL 1.0052 mL 2.0103 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    纯度 & 产品资料

    纯度: ≥98.0%

    染色示例
    参考文献
    Cell Assay
    [1][2][3]

    K562, Daudi, and Chang liver cells are labeled with calcein-AM. Calcein-AM's excitation and emission wavelengths are 496 nm and 520 nm, respectively. The filter/mirror combination used to detect calcein-AM's green fluorescence includes the 490-nm excitation and 520-nm emission filters with a dichroic mirror. Differences in the automatic fluorescence readings between the test and control wells determine the results[1]. A simple and sensitive cell-cell adhesion microplate assay is established using the calcein-AM. The procedure involves three steps: the labeling of lymphocytes with an adequate concentration of calcein-AM (20 μM) during a short incubation period (30 min); the adhesion of 2×105 labeled lymphocytes per well to confluent keratinocyte or fibroblast monolayers grown in microtiter plates for 90 min; and, finally, measurement of the fluorescent signal utilizing a new system of cold-light microfluorimetry[2]. Cells are incubated for 15 min in 1 mL of a 1% saponin solution in PBS buffer, pH 7.4, containing 0.05% sodium azide. After saponin permeabilization, 4×105 RBCs in suspension in PBS buffer containing 0.1% saponin and 0.05% sodium azide are incubated (37°C in the dark for 45 min) with calcein-AM to a final concentration of 5 μM, ished three times with the same PBS buffer containing 0.1% saponin and 0.05% sodium azide, and the cell viability is analyzed by flow cytometry[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    参考文献

    Calcein-AM 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.0052 mL 5.0258 mL 10.0517 mL 25.1292 mL
    5 mM 0.2010 mL 1.0052 mL 2.0103 mL 5.0258 mL
    10 mM 0.1005 mL 0.5026 mL 1.0052 mL 2.5129 mL
    15 mM 0.0670 mL 0.3351 mL 0.6701 mL 1.6753 mL
    20 mM 0.0503 mL 0.2513 mL 0.5026 mL 1.2565 mL
    25 mM 0.0402 mL 0.2010 mL 0.4021 mL 1.0052 mL
    30 mM 0.0335 mL 0.1675 mL 0.3351 mL 0.8376 mL
    40 mM 0.0251 mL 0.1256 mL 0.2513 mL 0.6282 mL
    50 mM 0.0201 mL 0.1005 mL 0.2010 mL 0.5026 mL
    60 mM 0.0168 mL 0.0838 mL 0.1675 mL 0.4188 mL
    80 mM 0.0126 mL 0.0628 mL 0.1256 mL 0.3141 mL
    100 mM 0.0101 mL 0.0503 mL 0.1005 mL 0.2513 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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