Cholera toxin  (Synonyms: 霍乱毒素; Choleragen)

Cholera toxin (Choleragen), an AB(5)-subunit toxin, enters host cells by binding the ganglioside GM1 at the plasma membrane (PM) and travels retrograde through the trans-Golgi Network into the endoplasmic reticulum (ER)^[1]. Choleragen activates adenylate cyclase by catalyzing ADP-ribosylation of Gs alpha, the stimulatory guanine nucleotide-binding protein^[2].

在 ER 中，Cholera toxin 的一部分，即酶促 A1 链，被蛋白质二硫键异构酶展开，并通过劫持错误折叠蛋白质的 ER 相关降解途径的组分逆向转运到胞质溶胶^[1]。
Cholera toxin 充当 ADP-核糖基转移酶，破坏靶细胞中的细胞内信号传导。Cholera toxin 通过囊泡载体从细胞表面移动到中毒细胞的内质网 (ER)^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

9012-63-9

固体

White to off-white

霍乱毒素

Room temperature in continental US; may vary elsewhere.

Store at 4°C, do not freeze

• [1]. Kellner A, et al. A binding motif for Hsp90 in the A chains of ADP-ribosylating toxins that move from the endoplasmic reticulum to the cytosol. Cell Microbiol. 2019;21(10):e13074.  [Content Brief]

  [2]. Tsai SC, et al. Stimulation of choleragen enzymatic activities by GTP and two soluble proteins purified from bovine brain. J Biol Chem. 1988;263(4):1768-1772.  [Content Brief]

  [3]. Wernick NL, et al. Cholera toxin: an intracellular journey into the cytosol by way of the endoplasmic reticulum. Toxins (Basel). 2010;2(3):310-325.  [Content Brief]