Dermaseptin  (Synonyms: 皮抑菌肽)

Dermaseptin, a peptide isolated from frog skin, exhibits potent antimicrobial activity against bacteria, fungi, and protozoa at micromolar concentration^[1].

Dermaseptin is a water-soluble, thermostable, and nonhemolytic peptide endowed with highly potent antimicrobial activity against pathogenic fungi at micromolar concentration. Circular dichroism spectra of dermaseptin in hydrophobic media indicated 80% alpha-helical conformation, and predictions of secondary structure suggested that dermaseptin can be configured as an amphiphatic alpha-helix spanning over residues 1-27, a structure that perturbs membrane functions regulating water flux^[1]. Dermaseptin exerts a lytic action upon bacteria, protozoa, yeasts, and filamentous fungi at micromolar concentrations. Molecular elements responsible for the exceptional antimicrobial potency of dermaseptin are to be traced to the NH2-terminal alpha-helical amphipathic segment spanning residues 1-18 of the molecule^[1].
Dermaseptin (5-100 μg/ml; 48 hours) inhibits by 100% the proliferation of most microorganisms tested, including Gram-positive or Gram-negative bacteria, parasites, yeasts, and filamentous fungi, at micromolar concentrations^[2].
Dermaseptin (5-100 μg/ml; 48 hours) does not inhibit the proliferation of human KJ3 cells after a 48 h incubation, and dermaseptin treatment for 1 h does not permeate guinea pig lymphocytes up to the highest concentration assayed (200 μg/ml). Hemolysis of rabbit erythrocytes occurrs after 1 h of treatment at doses above 200 μg/ml, with 50% hemolysis at 350 μg/ml^[2].
Dermaseptin has antimicrobial activities and is against Aeromonas cauiae, Pseudomonas aeroginusa, Escherichia coli, Enterococcus faecalis, L. mezicana (NF ^α strain) and Microsporum canis (IP1194) with MIC values of 50 μg/ml; 100 μg/ml; 25 μg/ml; 15 μg/ml; and 50 μg/ml, respectively^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

3455.10

C₁₅₂H₂₅₇N₄₃O₄₄S₂

136212-91-4

固体

White to off-white

Ala-Leu-Trp-Lys-Thr-Met-Leu-Lys-Lys-Leu-Gly-Thr-Met-Ala-Leu-His-Ala-Gly-Lys-Ala-Ala-Leu-Gly-Ala-Ala-Ala-Asp-Thr-Ile-Ser-Gln-Gly-Thr-Gln

ALWKTMLKKLGTMALHAGKAALGAAADTISQGTQ

皮抑菌肽

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

• [1]. Mor A, et al. Isolation, amino acid sequence, and synthesis of dermaseptin, a novel antimicrobial peptide of amphibian skin. Biochemistry. 1991 Sep 10;30(36):8824-30.  [Content Brief]

  [2]. Mor A, et al. The NH2-terminal alpha-helical domain 1-18 of dermaseptin is responsible for antimicrobial activity. J Biol Chem. 1994 Jan 21;269(3):1934-9.  [Content Brief]