1. Metabolic Enzyme/Protease
  2. Phosphatase
  3. EGF Receptor Substrate 2 (Phospho-Tyr5)

EGF Receptor Substrate 2 (Phospho-Tyr5) 

目录号: HY-P0320 纯度: 99.53%
COA 产品使用指南

EGF Receptor Substrate 2 (Phospho-Tyr5) acetate,一种生物活性肽,是一种酪氨酸磷酸底物。EGF Receptor Substrate 2 (Phospho-Tyr5) acetate 可用于检测蛋白酪氨酸磷酸酶活性。

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Custom Peptide Synthesis

EGF Receptor Substrate 2 (Phospho-Tyr5) Chemical Structure

EGF Receptor Substrate 2 (Phospho-Tyr5) Chemical Structure

CAS No. : 149261-42-7

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
1 mg ¥900
In-stock
5 mg ¥2600
In-stock
10 mg ¥4600
In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Customer Review

Other Forms of EGF Receptor Substrate 2 (Phospho-Tyr5):

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

EGF Receptor Substrate 2 (Phospho-Tyr5) acetate, a biologically active peptide, is a tyrosine phosphate substrate. EGF Receptor Substrate 2 (Phospho-Tyr5) acetate can be used to detect protein tyrosine phosphatases activity[1].

分子量

1328.28

Formula

C54H82N13O24P

CAS 号
性状

固体

颜色

White to off-white

Sequence

H-Asp-Ala-Asp-Glu-{pTyr}-Leu-Ile-Pro-Gln-Gln-Gly

Sequence Shortening

DADE-{pTyr}-LIPQQG

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O 中的溶解度 : 50 mg/mL (37.64 mM; 超声助溶)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.7529 mL 3.7643 mL 7.5285 mL
5 mM 0.1506 mL 0.7529 mL 1.5057 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳,请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度,如有需要,请与 MCE 中国技术支持联系。
纯度 & 产品资料
参考文献
Kinase Assay
[1]

Briefly, the assays are performed in 50 μL volumes in a microtiter plate using the generalized PTPase substrates ENDpYINASL and DADEpYLIPQQG (where pY represents phosphotyrosine) and assay buffer containing 25 mM Tris-HCl (pH 7.4), 50 mM NaCl, 2 mM EDTA, 5 mM dithiothreitol, 0.01% Brij 35, and 1 mg of bovine serum albumin/mL. The reactions are started by the addition of 5 μg of either CAV GST-VP2, GST, CAV GST-VP2 containing the C95S mutation, or TLMV GST-ORF2 or 2 units of the positive control T cell protein-tyrosine phosphatase (TC-PTP) in assay buffer. Control reactions are assayed with either ENDpYINASL substrate alone, DADEpYLIPQQG substrate alone, CAV GST-VP2 without substrate, CAV GST-VP2 containing the C95S mutation without substrate, TLMV GST-ORF2 without substrate, TC-PTP without substrate, or assay buffer with neither enzyme nor substrate. A phosphate standard curve is derived using a supplied phosphate standard. The reactions are terminated by the addition of the malachite green detection reagent.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

EGF Receptor Substrate 2 (Phospho-Tyr5) 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.7529 mL 3.7643 mL 7.5285 mL 18.8213 mL
5 mM 0.1506 mL 0.7529 mL 1.5057 mL 3.7643 mL
10 mM 0.0753 mL 0.3764 mL 0.7529 mL 1.8821 mL
15 mM 0.0502 mL 0.2510 mL 0.5019 mL 1.2548 mL
20 mM 0.0376 mL 0.1882 mL 0.3764 mL 0.9411 mL
25 mM 0.0301 mL 0.1506 mL 0.3011 mL 0.7529 mL
30 mM 0.0251 mL 0.1255 mL 0.2510 mL 0.6274 mL

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
EGF Receptor Substrate 2 (Phospho-Tyr5)
目录号:
HY-P0320
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