1. GPCR/G Protein Neuronal Signaling Membrane Transporter/Ion Channel Immunology/Inflammation Apoptosis
  2. 5-HT Receptor Potassium Channel Interleukin Related TNF Receptor
  3. Eucalyptol

Eucalyptol  (Synonyms: 1,8-Cineole)

目录号: HY-N0066 纯度: ≥98.0%
COA 产品使用指南

Eucalyptol 是 5-HT3 受体, 钾通道, TNF-αIL-1β 的抑制剂。

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Eucalyptol Chemical Structure

Eucalyptol Chemical Structure

CAS No. : 470-82-6

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Top Publications Citing Use of Products
  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Eucalyptol is an inhibitor of 5-HT3 receptor ,potassium channel, TNF-α and IL-1β.

IC50 & Target

5-HT3 Receptor

 

IL-1β

 

potassium channel

 

体外研究
(In Vitro)

Eucalyptol inhibits 5-HT-evoked currents in oocytes expressing 5-HT3 receptors with an IC50 of 258 µM[1]. Eucalyptol (Cin) treatment significantly decreases the ROS level in Aβ25-35 treated cells in a dose dependent manner. Eucalyptol treatment significantly decreases the NO level in Aβ25-35 treated cells in a dose dependent manner (p<0.05 and p<0.01). Eucalyptol treatment also significantly decreases IL-1βlevel in Aβ25-35 treated cells in a dose dependent manner (p<0.05 and p<0.01) as compare to Aβ25-35 treated PC12 cells. IL-6 level is also attenuated by Eucalyptol in dose dependent manner (p<0.05 and p<0.01) as compare to Aβ25-35 treated cells[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Results show that male and female rats treated with Eucalyptol (CIN) at the highest doses, 500 and 1000 mg/kg, have shown lower body weight than control group from the 7th to 50th day of treatment. The administration of Eucalyptol significantly reduces body weight gain of male rats (Eucalyptol 500 and 1000 mg/kg) and female rats (Eucalyptol 1000 mg/kg) in the first week of treatment. However, this reduction is followed by an increase in body weight of rats males and females treated with all doses of the second week until the end of treatment. For male rats, there is a significant increase of 6.93% in mean corpuscular volume (MCV) (Eucalyptol 1000 mg/kg) and of 43.54 and 38.98% in the platelet count (Eucalyptol 500 and 1000 mg/kg, respectively) and a decrease of 6.74 and 6.67% in mean corpuscular hemoglobin concentration (MCHC) (Eucalyptol 500 and 1000 mg/kg) and mean platelet volume (MPV) of 10.40, 10.60 and 15.73% (Eucalyptol 100, 500 and 1000 mg/kg, respectively), when compare to the control group[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

154.25

Formula

C10H18O

CAS 号
性状

液体

颜色

Colorless to light yellow

中文名称

桉油精;桉树脑

结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO 中的溶解度 : ≥ 120 mg/mL (777.96 mM; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

H2O 中的溶解度 : 33.33 mg/mL (216.08 mM; 超声助溶)

* "≥" means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 6.4830 mL 32.4149 mL 64.8298 mL
5 mM 1.2966 mL 6.4830 mL 12.9660 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

In Vivo:

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 3.5 mg/mL (22.69 mM); 澄清溶液

    此方案可获得 ≥ 3.5 mg/mL(饱和度未知)的澄清溶液。

    1 mL 工作液为例,取 100 μL 35.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;再向上述体系中加入 50 μL Tween-80,混合均匀;然后再继续加入 450 μL 生理盐水 定容至 1 mL

    生理盐水的配制:将 0.9 g 氯化钠,溶解于 ddH₂O 并定容至 100 mL,可以得到澄清透明的生理盐水溶液。
  • 方案 二

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 3.5 mg/mL (22.69 mM); 澄清溶液

    此方案可获得 ≥ 3.5 mg/mL(饱和度未知)的澄清溶液。

    1 mL 工作液为例,取 100 μL 35.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中,混合均匀。

    20% SBE-β-CD in Saline 的配制(4°C,储存一周):2 g SBE-β-CD(磺丁基醚 β-环糊精)粉末定容于 10 mL 的生理盐水中,完全溶解至澄清透明。
动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳,请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度,如有需要,请与 MCE 中国技术支持联系。
纯度 & 产品资料
参考文献
Cell Assay
[3]

The protective dose of Eucalyptol (Cineole) is determined by MTT dye-uptake method. In brief, cells (1×104 per well) are seeded in 96-well tissue culture plates and allowed to adhere for 24 h in CO2 incubator at 37°C. Cells are differentiated for the indicated time period. Thereafter, the medium is replaced with the medium containing Eucalyptol (0 to 10 μM) in different experiments for a period up to 24 h. Tetrazolium bromide salt (5 mg/mL of stock in PBS) 10 μL/well is added in 100 mL of cell suspension and plate is incubated for 4 h. At the end of incubation period, the reaction mixture is carefully taken out and 200 μL of DMSO is added to each well by pipetting up and down several times until the content gets homogenized. The plates are kept on rocker shaker for 10 min at room temperature and then read at 550 nm using microplate reader[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[4]

Swiss mice are used in this experiment. The animals are randomly divided into two groups (n=5) and fasted overnight with free access to water. The group control receives a 1% Tween-80 aqueous solution (0.1 mL/10 g) and the other group is treated with Eucalyptol a single 2000 mg/kg dose by oral route. The animals are observed at 30, 60, 120, 180 and 240 min after oral treatment and daily for 14 days. Behavioral changes, weight, food and water consumption, clinical signs of toxicity or mortality are recorded daily[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 6.4830 mL 32.4149 mL 64.8298 mL 162.0746 mL
5 mM 1.2966 mL 6.4830 mL 12.9660 mL 32.4149 mL
10 mM 0.6483 mL 3.2415 mL 6.4830 mL 16.2075 mL
15 mM 0.4322 mL 2.1610 mL 4.3220 mL 10.8050 mL
20 mM 0.3241 mL 1.6207 mL 3.2415 mL 8.1037 mL
25 mM 0.2593 mL 1.2966 mL 2.5932 mL 6.4830 mL
30 mM 0.2161 mL 1.0805 mL 2.1610 mL 5.4025 mL
40 mM 0.1621 mL 0.8104 mL 1.6207 mL 4.0519 mL
50 mM 0.1297 mL 0.6483 mL 1.2966 mL 3.2415 mL
60 mM 0.1080 mL 0.5402 mL 1.0805 mL 2.7012 mL
80 mM 0.0810 mL 0.4052 mL 0.8104 mL 2.0259 mL
100 mM 0.0648 mL 0.3241 mL 0.6483 mL 1.6207 mL

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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目录号:
HY-N0066
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