1. Peptides
  2. FLAG peptide

FLAG peptide 是一种八个氨基酸组成的多肽 (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys),具有肠激酶切割位点。FLAG peptide 用于抗体介导的重组蛋白的识别和纯化。

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Custom Peptide Synthesis

FLAG peptide Chemical Structure

FLAG peptide Chemical Structure

CAS No. : 98849-88-8

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
1 mg ¥500
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5 mg ¥900
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10 mg ¥1300
In-stock
25 mg ¥2390
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100 mg   询价  

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Top Publications Citing Use of Products
  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

FLAG peptide is an eight amino acids peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) with an enterokinase-cleavage site; designed for antibody-mediated identification and purification of recombinant proteins.

体外研究
(In Vitro)

Fusion protein technology has become an important tool for solving numerous problems linked to recombinant protein production. The properties of the additional tag facilitate identification and provide a one-step purification procedure of the fusion protein by passing cell extracts or supernatants through columns of an appropriate matrix. FLAG peptide allows elution under non-denaturing conditions. Several antibodies against FLAG peptide have been developed. One antibody, M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free FLAGe peptide. Antibodies M2 and M5 are applied in this procedure[1]. The Flag-tag is first described as a calcium-dependent epitope of a monoclonal antibody. It is a highly acidic octapeptide which can be N-terminally fused to the protein of interest. As a very hydrophilic peptide the Flag–tag has a high surface probability. Flag-fusion proteins can be captured by an immunoaffinity column in the presence of Ca2+ and eluted byEDTA at low concentrations, neutral pH and thus, nearly physiological conditions[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1012.97

Formula

C41H60N10O20

CAS 号
性状

固体

颜色

White to off-white

Sequence

Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys

Sequence Shortening

DYKDDDDK

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture and light, under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

溶解性数据
In Vitro: 

H2O 中的溶解度 : 100 mg/mL (98.72 mM; 超声助溶)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.9872 mL 4.9360 mL 9.8720 mL
5 mM 0.1974 mL 0.9872 mL 1.9744 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

In Vivo:

以下溶解方案,请直接配置工作液。建议现用现配,在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比; 如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶。

  • 方案 一

    请依序添加每种溶剂: PBS

    Solubility: 100 mg/mL (98.72 mM); 澄清溶液; 超声助溶

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳,请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度,如有需要,请与 MCE 中国技术支持联系。
纯度 & 产品资料

纯度: 99.51%

参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.9872 mL 4.9360 mL 9.8720 mL 24.6799 mL
5 mM 0.1974 mL 0.9872 mL 1.9744 mL 4.9360 mL
10 mM 0.0987 mL 0.4936 mL 0.9872 mL 2.4680 mL
15 mM 0.0658 mL 0.3291 mL 0.6581 mL 1.6453 mL
20 mM 0.0494 mL 0.2468 mL 0.4936 mL 1.2340 mL
25 mM 0.0395 mL 0.1974 mL 0.3949 mL 0.9872 mL
30 mM 0.0329 mL 0.1645 mL 0.3291 mL 0.8227 mL
40 mM 0.0247 mL 0.1234 mL 0.2468 mL 0.6170 mL
50 mM 0.0197 mL 0.0987 mL 0.1974 mL 0.4936 mL
60 mM 0.0165 mL 0.0823 mL 0.1645 mL 0.4113 mL
80 mM 0.0123 mL 0.0617 mL 0.1234 mL 0.3085 mL

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Inquiry Information

产品名称:
FLAG peptide
目录号:
HY-P0223
需求量: