1. Apoptosis Anti-infection
  2. Ferroptosis Fungal
  3. Ferrostatin-1

Ferrostatin-1  (Synonyms: Fer-1)

目录号: HY-100579 纯度: 99.96%
COA 产品使用指南

Ferrostatin-1 (Fer-1) 是一种有效的、选择性的 ferroptosis 抑制剂,抑制 Erastin 诱导的 HT-1080 细胞铁死亡 (EC50=60 nM)。Ferrostatin-1 是一种人工合成的抗氧化剂,通过还原机制来防止膜脂的损伤,从而抑制细胞死亡。Ferrostatin-1 具有抗真菌活性。

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Ferrostatin-1 Chemical Structure

Ferrostatin-1 Chemical Structure

CAS No. : 347174-05-4

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥726
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5 mg ¥660
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10 mg ¥1150
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25 mg ¥2450
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50 mg ¥4200
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100 mg ¥6300
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Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 501 篇科研文献

WB
Cell Viability Assay
Proliferation Assay
IF

    Ferrostatin-1 purchased from MCE. Usage Cited in: J Funct Foods. 2023 May.

    Ferrostatin-1 (Fer-1) significantly restores the decreased cell viability in HK-1 and 5-8F cells induced by α-solanine.

    Ferrostatin-1 purchased from MCE. Usage Cited in: Sleep Breath. 2023 Mar 1.  [Abstract]

    The protein content of GPX4 and FTH1 is signifcantly upregulated in intermittent hypoxia (IH)+ferrostatin-1 (5 μM) group, in rat liver (BRL‑3A) cells.

    Ferrostatin-1 purchased from MCE. Usage Cited in: Cell Discov. 2022 May 3;8(1):40.  [Abstract]

    U2OS cells are incubated with DMSO or OSMI-1 for 12 h and then pre-treated with Ferrostatin-1 (Fer-1; 2 μM) for 2 h before the treatment of RSL3 for 4 h. Cells are stained with PI and cell death is then assessed by flow cytometry.

    Ferrostatin-1 purchased from MCE. Usage Cited in: Redox Biol. 2022 Oct;56:102435.  [Abstract]

    Primary HSCs and LX-2 cells are treated with EA (LX-2 cells: 40 μM; primary HSCs: 45 μM), Ferrostatin-1 (1 μM) and ZVAD-FMK (10 μM), and cells are stained with PI (red fluorescence) to examine the dead cells.

    Ferrostatin-1 purchased from MCE. Usage Cited in: EBioMedicine. 2022 Feb;76:103847.  [Abstract]

    Mitochondrial membrane potential (ΔΨm) measured by fluorescence microscopy of MCC treated with TBHP and rescued via Ferrostatin-1 (Fer-1; 1 μM) using JC-1 Mitochondrial Membrane Potential Assay. In Fer-1 and DFO group, red fluorescence (poly JC-1) is increased and green fluorescence (mono JC-1) is decreased.

    Ferrostatin-1 purchased from MCE. Usage Cited in: Bioact Mater. 2021 Nov 19;13:23-36.  [Abstract]

    H1299 and H460 cells are treated with Curcumenol with or without Ferrostatin-1 (Fer-1; 1 μM) for 24 h, and the cell viability is analyzed.

    Ferrostatin-1 purchased from MCE. Usage Cited in: J Exp Clin Cancer Res. 2021 Jun 23;40(1):206.  [Abstract]

    T47D cells are treated with Metformin (5 mM) for 48 h in the absence or presence of Ferrostatin-1 (1 μM) for 48 h.

    Ferrostatin-1 purchased from MCE. Usage Cited in: Cancer Lett. 2021 Jul 10;510:93-104.  [Abstract]

    Ferrostatin-1(2 μM) has the ability to fully restore the cell viability impaired by Erastin (5 μM) treated control cells in GBC-SD cells.

    Ferrostatin-1 purchased from MCE. Usage Cited in: Int J Biol Sci. 2021 Mar 1;17(4):942-956.  [Abstract]

    Gallic acid affects the expression of ferroptosis-related proteins. The relative expression levels of GPX4, SLC7A11, and SIGMAR1 decreased, while the relative expression levels of ATF4 and TFR1 increased. Ferrostatin-1 (2 μM) as a ferroptosis inhibitor reverses the expression trend of these proteins.

    Ferrostatin-1 purchased from MCE. Usage Cited in: Int J Biol Sci. 2021 Mar 1;17(4):942-956.  [Abstract]

    After the gallic acid treatment, Fe2+, ROS, and MDA are significantly increased compared to the control group, and at the same time, the content of GSH is significantly reduced. The intervention of Ferrostatin-1 (2 μM) while treating the cells with gallic acid reversed the changes in these indicators to varying degrees.

    Ferrostatin-1 purchased from MCE. Usage Cited in: Signal Transduct Target Ther. 2020 May 8;5(1):51.  [Abstract]

    Erastin-induced cell death in lung cancer cells is almost blocked by cotreatment with the Ferrostatin-1 (Fer-1; 24 h) in H460 and H1299 cells.

    Ferrostatin-1 purchased from MCE. Usage Cited in: Theranostics. 2020 Aug 2;10(21):9865-9887.  [Abstract]

    p53 expression of HepG2 cells after treated with Fer-1 at a concentration of 0-40 nM. The addition of Fer-1 could repress the expression of p53 protein by inhibiting the ferroptosis of SFB formulation.

    Ferrostatin-1 purchased from MCE. Usage Cited in: Cell Death Dis. 2020 Sep 15;11(9):756.  [Abstract]

    Acot1 protein levels in different concentrations of DOX-treated HL-1 cell with or without Fer-1 (10 μM) for 24 h. Acot1 expression is decreased at a higher concentration of DOX, while Fer-1 co-treatment partially suppressed this trend.
    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Ferrostatin-1 (Fer-1), a potent and selective ferroptosis inhibitor, suppresses Erastin-induced ferroptosis in HT-1080 cells (EC50=60 nM). Ferrostatin-1, a synthetic antioxidant, acts via a reductive mechanism to prevent damage to membrane lipids and thereby inhibits cell death. Ferrostatin-1 exhibits antifungal activity[1][2][3].

    IC50 & Target

    EC50: 60 nM (Ferroptosis)[1]

    体外研究
    (In Vitro)

    Ferrostatin-1 可防止 erastin 诱导的细胞溶质和脂质 ROS 积累。 Ferrostatin-1 可预防器官型大鼠脑切片中谷氨酸诱导的神经毒性[1]
    Ferrostatin-1(2 μM;24 小时)可防止大鼠器官型海马切片培养物 (OHSC) 中谷氨酸 (5 mM) 诱导的神经毒性[2]
    Ferrostatin-1 抑制脂质过氧化,但不抑制线粒体活性氧的形成或溶酶体膜通透性[2]
    Ferrostatin-1 在亨廷顿病 (HD)、脑室周围白质软化症 (PVL) 和肾功能障碍的细胞模型中抑制细胞死亡[2]
    Ferrostatin-1(1 μM;6 小时)抑制 HT-1080 细胞中不饱和脂肪酸的氧化破坏,从而增加健康的中型多棘神经元 (MSN) 的数量[3]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    体内研究
    (In Vivo)

    Ferrostatin-1(5 mg/kg;腹腔注射;单剂量,处理于甘油注射前 30 分钟)改善横纹肌溶解小鼠的肾功能,而泛半胱天冬酶抑制剂 zVAD 或 RIPK3 缺陷小鼠未观察到有益效果 [1].
    Ferrostatin-1(0.8 mg/kg;尾静脉注射)有效缓解LPS诱导的急性肺损伤(ALI)[4]
    Ferrostatin-1(5 mg/kg;腹腔注射;C57BL/6J 小鼠)改善横纹肌溶解症小鼠的肾功能[5]
    Ferrostatin-1 (10 mg/kg/d, 腹腔注射, 3 d) 可在新生大鼠的大脑中减轻缺氧缺血性脑损伤-、氧葡萄糖剥夺-或 Erastin (HY-15763)-诱导的铁死亡[6]
    Ferrostatin-1 (0.655 mg/kg, 腹腔注射, 一周三次,共六周) 通过增加 GPX4 活性和抑制脂质过氧化在卵巢切除 (绝经后模型) 大鼠唾液腺中发挥抗铁死亡作用[7]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male C57BL/6 mice (LPS-induced ALI)[4]
    Dosage: 0.8 mg/kg
    Administration: Tail vein injection
    Result: Exerted therapeutic action against LPS-induced ALI.
    分子量

    262.35

    Formula

    C15H22N2O2

    CAS 号
    性状

    固体

    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    溶解性数据
    In Vitro: 

    DMSO 中的溶解度 : 125 mg/mL (476.46 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 3.8117 mL 19.0585 mL 38.1170 mL
    5 mM 0.7623 mL 3.8117 mL 7.6234 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    In Vivo:

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
    ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (9.53 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL(饱和度未知)的澄清溶液。

      1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;再向上述体系中加入 50 μL Tween-80,混合均匀;然后再继续加入 450 μL 生理盐水 定容至 1 mL

      生理盐水的配制:将 0.9 g 氯化钠,溶解于 ddH₂O 并定容至 100 mL,可以得到澄清透明的生理盐水溶液。
    • 方案 二

      请依序添加每种溶剂: 10% DMSO    90% Corn Oil

      Solubility: 2.5 mg/mL (9.53 mM); 悬浊液; 超声助溶

      此方案可获得 2.5 mg/mL的均匀悬浊液,悬浊液可用于口服和腹腔注射。

      1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    请输入您的动物体内配方组成:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    如果您的动物是免疫缺陷鼠或者体弱鼠,建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
    方案所需 助溶剂 包括:DMSO ,均可在 MCE 网站选购。 Tween 80,均可在 MCE 网站选购。
    计算结果
    工作液所需浓度 : mg/mL
    储备液配制方法 : mg 药物溶于 μL  DMSO(母液浓度为 mg/mL)。

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    您所需的储备液浓度超过该产品的实测溶解度,以下方案仅供参考,如有需要,请与 MCE 中国技术支持联系。
    动物实验体内工作液的配制方法 : 取 μL DMSO 储备液,加入 μL  μL ,混合均匀至澄清,再加 μL Tween 80,混合均匀至澄清,再加 μL 生理盐水
    连续给药周期超过半月以上,请谨慎选择该方案。
    请确保第一步储备液溶解至澄清状态,从左到右依次添加助溶剂。您可采用超声加热 (超声清洗仪,建议频次 20-40 kHz),涡旋吹打等方式辅助溶解。
    纯度 & 产品资料

    纯度: 99.96%

    参考文献

    Ferrostatin-1 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.8117 mL 19.0585 mL 38.1170 mL 95.2925 mL
    5 mM 0.7623 mL 3.8117 mL 7.6234 mL 19.0585 mL
    10 mM 0.3812 mL 1.9059 mL 3.8117 mL 9.5293 mL
    15 mM 0.2541 mL 1.2706 mL 2.5411 mL 6.3528 mL
    20 mM 0.1906 mL 0.9529 mL 1.9059 mL 4.7646 mL
    25 mM 0.1525 mL 0.7623 mL 1.5247 mL 3.8117 mL
    30 mM 0.1271 mL 0.6353 mL 1.2706 mL 3.1764 mL
    40 mM 0.0953 mL 0.4765 mL 0.9529 mL 2.3823 mL
    50 mM 0.0762 mL 0.3812 mL 0.7623 mL 1.9059 mL
    60 mM 0.0635 mL 0.3176 mL 0.6353 mL 1.5882 mL
    80 mM 0.0476 mL 0.2382 mL 0.4765 mL 1.1912 mL
    100 mM 0.0381 mL 0.1906 mL 0.3812 mL 0.9529 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    产品名称:
    Ferrostatin-1
    目录号:
    HY-100579
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