1. Epigenetics TGF-beta/Smad
  2. PKC
  3. Go 6983

Go 6983  (Synonyms: Gö 6983; Goe 6983)

目录号: HY-13689 纯度: 98.42%
COA 产品使用指南

Go 6983 是一种有效的 PKC 抑制剂,能够作用于 PKCαPKCβPKCγPKCδPKCζIC50 值分别为 7 nM,7 nM,6 nM,10 nM 和 60 nM。

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Go 6983 Chemical Structure

Go 6983 Chemical Structure

CAS No. : 133053-19-7

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥928
In-stock
5 mg ¥844
In-stock
10 mg ¥1350
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50 mg ¥4150
In-stock
100 mg ¥6640
In-stock
200 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

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Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 48 篇科研文献

WB
RT-PCR
IF

    Go 6983 purchased from MCE. Usage Cited in: Cell Death Dis. 2018 Feb 7;9(2):165.  [Abstract]

    Western blot analysis of MCF-7 cells treated with Tan IIA at the specified concentrations for the expression of cytosolic PKC isoforms. The compound Go 6983 (GO, 20 μM) is included as a positive control.

    Go 6983 purchased from MCE. Usage Cited in: Biochem Biophys Res Commun. 2018 Sep 3;503(1):62-70.  [Abstract]

    BMMs are incubated in serum-free medium and pretreated with or without Go6983 (0, 2.5 μM) for 4 h. Next, the BMMs are stimulated by RANKL (50 ng/mL) for the indicated times (0, 15, 30, 60 min). Additionally, the BMM lysates are analyzed by western blotting.

    Go 6983 purchased from MCE. Usage Cited in: J Appl Toxicol. 2016 Nov;36(11):1409-17.   [Abstract]

    Protein levels of GLT-1 analyzed by Western blotting in C6 cells then treated with TCDD for 12 h. Cells are pre-treated with Ca2+-signaling blocker BAY-a-1040 (25 nM) for 30min before TCDD stimulation. The PKC inhibitor staurosporine (10 nM) and Go 6983 (1 μM) is applied 2 h before adding TCDD.

    Go 6983 purchased from MCE. Usage Cited in: J Appl Toxicol. 2016 Nov;36(11):1409-17.   [Abstract]

    Quantitative analysis of the intensity of GLT-1 expression relative to GAPDH in the indicated groups. Cells are pre-treated with Ca2+-signaling blocker BAY-a-1040 (25 nM) for 30min before TCDD stimulation. The PKC inhibitor staurosporine (10 nM) and Go 6983 (1 μM) is applied 2 h before adding TCDD.

    Go 6983 purchased from MCE. Usage Cited in: J Appl Toxicol. 2016 Nov;36(11):1409-17.   [Abstract]

    Immunofluorescence staining of PKC after pre-treatment with the Ca2+-signaling blocker and the PKC inhibitor. PKC is enriched in cytoplasmor perinucleus in untreated C6 cells (a-c), Go 6983-treated cells (g-i) or BAY-a-1040-treated cells (m-o). TCDD induces PKC translocation to the membrane in C6 glioma cells (d-f ).

    Go 6983 purchased from MCE. Usage Cited in: Vet Microbiol. 2016 Aug 30;192:26-33.  [Abstract]

    Effect of PKC inhibitor on NF-kB DNA binding activity and NFAT inhibitor on NFATc1 DNA binding activity. Lane N is negative control; lane 1 is control group; lane 2 is S. uberis group; lane 3 is pre-treated with 2 μM Go 6983; lane 4 is pre-treated with 5 μM Go 6983; lane 5 is pre-treated with 10 μM Go 6983.
    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Go 6983 is a pan-PKC inhibitor against for PKCα, PKCβ, PKCγ, PKCδ and PKCζ with IC50 of 7 nM, 7 nM, 6 nM, 10 nM and 60 nM, respectively.

    IC50 & Target[1]

    PKCγ

    6 nM (IC50)

    PKCα

    7 nM (IC50)

    PKCβ

    7 nM (IC50)

    PKCδ

    10 nM (IC50)

    PKCζ

    60 nM (IC50)

    PKCμ

    20000 nM (IC50)

    体外研究
    (In Vitro)

    Go 6983 抑制 PKCu,IC50 为 20 μM,PKC 同工酶可被 Go 6983 抑制,IC50 值为 7 至 60 nM[1]。与 I/R + PMN 心脏相比,Go 6983 (100 nM) 显著降低 PMN 对内皮细胞的粘附和对心肌的浸润,并且在大鼠心脏中显著抑制 PMN 超氧化物释放 90 +/-2%[2]。尽管可抑制 99% 的 PMN 超氧化物释放,但与具有心脏保护作用的 Go 6983 浓度 (50 和 100 nM) 相比,Go 6983 (200 nM) 的心脏保护作用有所降低[3]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    分子量

    442.51

    Formula

    C26H26N4O3

    CAS 号
    性状

    固体

    颜色

    Pink to red

    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    溶解性数据
    In Vitro: 

    DMSO 中的溶解度 : ≥ 34 mg/mL (76.83 mM; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

    * "≥" means soluble, but saturation unknown.

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 2.2598 mL 11.2992 mL 22.5984 mL
    5 mM 0.4520 mL 2.2598 mL 4.5197 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    In Vivo:

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
    ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (5.65 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL(饱和度未知)的澄清溶液。

      1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;再向上述体系中加入 50 μL Tween-80,混合均匀;然后再继续加入 450 μL 生理盐水 定容至 1 mL

      生理盐水的配制:将 0.9 g 氯化钠,溶解于 ddH₂O 并定容至 100 mL,可以得到澄清透明的生理盐水溶液。
    • 方案 二

      请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2.5 mg/mL (5.65 mM); 悬浊液; 超声助溶

      此方案可获得 2.5 mg/mL的均匀悬浊液,悬浊液可用于口服和腹腔注射。

      1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中,混合均匀。

      20% SBE-β-CD in Saline 的配制(4°C,储存一周):2 g SBE-β-CD(磺丁基醚 β-环糊精)粉末定容于 10 mL 的生理盐水中,完全溶解至澄清透明。
    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    请输入您的动物体内配方组成:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    如果您的动物是免疫缺陷鼠或者体弱鼠,建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
    方案所需 助溶剂 包括:DMSO ,均可在 MCE 网站选购。 Tween 80,均可在 MCE 网站选购。
    计算结果
    工作液所需浓度 : mg/mL
    储备液配制方法 : mg 药物溶于 μL  DMSO(母液浓度为 mg/mL)。
    您所需的储备液浓度超过该产品的实测溶解度,以下方案仅供参考,如有需要,请与 MCE 中国技术支持联系。
    动物实验体内工作液的配制方法 : 取 μL DMSO 储备液,加入 μL  μL ,混合均匀至澄清,再加 μL Tween 80,混合均匀至澄清,再加 μL 生理盐水
    连续给药周期超过半月以上,请谨慎选择该方案。
    请确保第一步储备液溶解至澄清状态,从左到右依次添加助溶剂。您可采用超声加热 (超声清洗仪,建议频次 20-40 kHz),涡旋吹打等方式辅助溶解。
    纯度 & 产品资料

    纯度: 98.42%

    参考文献
    Kinase Assay
    [1]

    Phosphorylation reactions are carried out in a total volume of 100 μL, containing buffer C (50 mM Tris-HCl, pH 7.5, 10 mM β-mercaptoethanol), 4 mM MgCl2, 10 μg PS, 100 nM TPA, 5 μL of a Sf158 cell extract as a source of recombinant PKCμ or of Sf9 cell extracts as a source of other recombinant PKC isoenzymes, 10 μg of syntide 2 as substrate, and 35 μM ATP containing 1 μCi [γ-32P]ATP. In some experiments, PS and TPA are omitted or various inhibitors at concentrations indicated in the text are added. After incubation for 10 min at 30°C, the reaction is terminated by transferring 50 μL of the assay mixture onto a 20 mm square piece of phosphocellulose paper, which is washed 3 times in deionized water and twice in acetone. The radioactivity on each paper is determined by liquid scintillation counting.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    参考文献

    Go 6983 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.2598 mL 11.2992 mL 22.5984 mL 56.4959 mL
    5 mM 0.4520 mL 2.2598 mL 4.5197 mL 11.2992 mL
    10 mM 0.2260 mL 1.1299 mL 2.2598 mL 5.6496 mL
    15 mM 0.1507 mL 0.7533 mL 1.5066 mL 3.7664 mL
    20 mM 0.1130 mL 0.5650 mL 1.1299 mL 2.8248 mL
    25 mM 0.0904 mL 0.4520 mL 0.9039 mL 2.2598 mL
    30 mM 0.0753 mL 0.3766 mL 0.7533 mL 1.8832 mL
    40 mM 0.0565 mL 0.2825 mL 0.5650 mL 1.4124 mL
    50 mM 0.0452 mL 0.2260 mL 0.4520 mL 1.1299 mL
    60 mM 0.0377 mL 0.1883 mL 0.3766 mL 0.9416 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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