1. Immunology/Inflammation
  2. IRAK
  3. IRAK-1-4 Inhibitor I

IRAK-1-4 Inhibitor I  (Synonyms: IRAK-1/4 Inhibitor I)

目录号: HY-13329 纯度: 99.78%
COA 产品使用指南

IRAK-1-4 Inhibitor I 是 IRAK1/4 抑制剂,IC50 分别为 0.2 μM 和 0.3 μM。

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IRAK-1-4 Inhibitor I Chemical Structure

IRAK-1-4 Inhibitor I Chemical Structure

CAS No. : 509093-47-4

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1 mg ¥376
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5 mg ¥828
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10 mg ¥1135
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50 mg ¥3800
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100 mg ¥5400
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Top Publications Citing Use of Products

    IRAK-1-4 Inhibitor I purchased from MCE. Usage Cited in: Oncogene. 2020 Sep;39(36):5888-5901.  [Abstract]

    The indicated cells are treated with IRAK1/4 inhibitor for 48 h. Nucleoplasm and chromatin-bound fractions are purified and immunoblots of the indicated antibodies are analyzed. Upon IRAK1/4 inhibitor treatment, while both chromatin-bound and free nucleoplasmic MLL are proportionally increased in the parental cells, only nucleoplasmic MLL is increased in the resistant cells.

    IRAK-1-4 Inhibitor I purchased from MCE. Usage Cited in: Antibiotics. 2020 Jan 17;9(1):33.  [Abstract]

    Huh7.5.1 cells are infected with J399EM at an MOI of 0.1, and then treated with different concentrations of IRAK-1/4 inhibitor I for 72 h. The expression level of the HCV core protein is determined by western blotting. The IRAK-1/4 inhibitor I is dissolved in dimethyl sulfoxide (DMSO) and the DMSO without IRAK-1/4 inhibitor I is used as a negative control.

    查看 IRAK 亚型特异性产品:

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    IRAK-1-4 Inhibitor I is an inhibitor of interleukin-1 receptor-associated kinase 1/4 (IRAK 1/4) with IC50s of 0.2 μM and 0.3 μM, respectively.

    IC50 & Target

    IC50: 0.2 μM (IRAK-4), 0.3 μM (IRAK-1)[1]

    体外研究
    (In Vitro)

    IRAK-1-4 Inhibitor I 的 IC50 高于测试的最高浓度 (10 μM),对其他 27 个激酶,包括最接近同源的 (IRAK家族之外的) Lck 和 pp60SRC。此外,在 HeLa 细胞的 72 h 增殖试验中,IRAK-1-4 Inhibitor I 未显示出任何细胞毒性迹象 (ED50>30 μM)。IRAK-1-4 Inhibitor I (IRAK-1 IC50=0.3 μM) 显著抑制 IRAK-1[1]。IRAK-1-4 Inhibitor I 可消除 LPS 诱导的 Bcl10、NF-κB 和 IL-8 的升高。IRAK-1-4 Inhibitor I 介导 LPS 诱导的 IL-8 激活并在 Bcl10 上游发挥作用。使 LPS 诱导的 Bcl10 增加发生 73% 的下降 (从 5.18±0.22 降至 2.36±0.08 ng/mL), IL-8 反应下降 60% (从2.64±0.31 降至 1.14±0.08 ng/mL)[2]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    分子量

    395.41

    Formula

    C20H21N5O4

    CAS 号
    性状

    固体

    颜色

    Light yellow to yellow

    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    溶解性数据
    In Vitro: 

    DMF 中的溶解度 : 25 mg/mL (63.23 mM; 超声助溶 (<60°C))

    DMSO 中的溶解度 : 5 mg/mL (12.65 mM; 超声助溶; 酸性条件溶解 (HCL 调节,pH≈3); 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

    H2O 中的溶解度 : < 0.1 mg/mL (insoluble)

    Ethanol 中的溶解度 : < 1 mg/mL (insoluble)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 2.5290 mL 12.6451 mL 25.2902 mL
    5 mM 0.5058 mL 2.5290 mL 5.0580 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 2 years; -20°C, 1 year。-80°C储存时,请在2年内使用, -20°C储存时,请在1年内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    纯度 & 产品资料

    纯度: 99.88%

    参考文献
    Cell Assay
    [2]

    NCM460 cells, grown in 24-well plates, are incubated with 50 μM IRAK-1/4 inhibitor for 2 h. After 2 h, the media are changed, and new media with or without LPS (10 ng/mL) added. Treatment is terminated at 6 h, and spent media and cells are collected for IL-8 and other assays[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    参考文献

    IRAK-1-4 Inhibitor I 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 2 years; -20°C, 1 year。-80°C储存时,请在2年内使用, -20°C储存时,请在1年内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO / DMF 1 mM 2.5290 mL 12.6451 mL 25.2902 mL 63.2255 mL
    5 mM 0.5058 mL 2.5290 mL 5.0580 mL 12.6451 mL
    10 mM 0.2529 mL 1.2645 mL 2.5290 mL 6.3226 mL
    DMF 15 mM 0.1686 mL 0.8430 mL 1.6860 mL 4.2150 mL
    20 mM 0.1265 mL 0.6323 mL 1.2645 mL 3.1613 mL
    25 mM 0.1012 mL 0.5058 mL 1.0116 mL 2.5290 mL
    30 mM 0.0843 mL 0.4215 mL 0.8430 mL 2.1075 mL
    40 mM 0.0632 mL 0.3161 mL 0.6323 mL 1.5806 mL
    50 mM 0.0506 mL 0.2529 mL 0.5058 mL 1.2645 mL
    60 mM 0.0422 mL 0.2108 mL 0.4215 mL 1.0538 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    产品名称:
    IRAK-1-4 Inhibitor I
    目录号:
    HY-13329
    需求量: