1. GPCR/G Protein Neuronal Signaling Membrane Transporter/Ion Channel
  2. Adrenergic Receptor iGluR
  3. Isoxsuprine hydrochloride

Isoxsuprine hydrochloride  (Synonyms: 去氧肾上腺素杂质31)

目录号: HY-B1270 纯度: 99.87%
COA 产品使用指南

Isoxsuprine hydrochloride 是 β 肾上腺素受体激动剂,对于子宫肌层和胎盘的 β 肾上腺素受体的 Ki 值分别为 13.65 μM 和 3.48 μM。Isoxsuprine hydrochloride 也是一种 NMDA 受体拮抗剂。

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Isoxsuprine hydrochloride Chemical Structure

Isoxsuprine hydrochloride Chemical Structure

CAS No. : 579-56-6

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥660
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200 mg ¥500
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1 g   询价  

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Other Forms of Isoxsuprine hydrochloride:

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  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Isoxsuprine hydrochloride is a beta-adrenergic receptor agonist with Kis of 13.65 μΜ and 3.48 μΜ for myometrial and placcntal beta-adrenergic receptor, respectively. Isoxsuprine hydrochloride is also a NMDA receptor antagonist.

IC50 & Target

NMDA Receptor

 

体外研究
(In Vitro)

Results show that Isoxsuprine hydrochloride inhibits circular chemorepellent induced defect (CCID) formation dose dependently (5 to 60 μM) and also inhibits 12(S)-HETE synthesis. Furthermore, Isoxsuprine hydrochloride is the only drug inhibiting the induction of all three mobility markers (MLC2, MYPT and paxillin)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Total infarct volume in vehicle-treated animals is 279±25 mm3 compare to 137±18 mm3 in Isoxsuprine hydrochloride-treated animals[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial
分子量

337.84

Formula

C18H24ClNO3

CAS 号
性状

固体

颜色

White to off-white

中文名称

去氧肾上腺素杂质31

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

DMSO 中的溶解度 : 140 mg/mL (414.40 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

H2O 中的溶解度 : 15.56 mg/mL (46.06 mM; 超声助溶)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.9600 mL 14.7999 mL 29.5998 mL
5 mM 0.5920 mL 2.9600 mL 5.9200 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

In Vivo:

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 3.5 mg/mL (10.36 mM); 澄清溶液

    此方案可获得 ≥ 3.5 mg/mL(饱和度未知)的澄清溶液。

    1 mL 工作液为例,取 100 μL 35.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;再向上述体系中加入 50 μL Tween-80,混合均匀;然后再继续加入 450 μL 生理盐水 定容至 1 mL

    生理盐水的配制:将 0.9 g 氯化钠,溶解于 ddH₂O 并定容至 100 mL,可以得到澄清透明的生理盐水溶液。
  • 方案 二

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 3.5 mg/mL (10.36 mM); 澄清溶液

    此方案可获得 ≥ 3.5 mg/mL(饱和度未知)的澄清溶液。

    1 mL 工作液为例,取 100 μL 35.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中,混合均匀。

    20% SBE-β-CD in Saline 的配制(4°C,储存一周):2 g SBE-β-CD(磺丁基醚 β-环糊精)粉末定容于 10 mL 的生理盐水中,完全溶解至澄清透明。
动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳,请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度,如有需要,请与 MCE 中国技术支持联系。
纯度 & 产品资料
参考文献
Cell Assay
[2]

MCF-7ALOX12 cells are seeded in 3.5-cm dishes and grown in 2.5 mL complete MEM medium without selection pressure. The next day, the medium is changed to serum-free medium and cells are kept at 37°C for 24 h. Then, cells are treated with 10 μM arachidonic acid and simultaneously with different concentrations of Isoxsuprine hydrochloride for 4 h when the supernatants are aspirated, centrifuged at 2000, r.p.m. at 4°C for 5 min, collected in cryo-tubes, flash frozen and stored at -80 °C until analysis[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[3]

Male spontaneously hypertensive rats (SHR) weighing 290 to 300 g are used in this study. At reperfusion, animals receive 0.5 mL of vehicle (0.6% DMSO in normal saline) or 1 mg/kg Isoxsuprine hydrochloride by intravenous (IV) injection through the lateral tail vein. All animals receive 3 mL of subcutaneous saline after surgery to prevent dehydration. After 24 hours reperfusion, animals are sacrificed, brains are sectioned into 4 mm-thick quadrants, and infarcted tissue is identified by 2,3,5-triphenyltetrazolium chloride (TTC) staining. Edema-corrected infarct volume is calculated by subtracting the area of non-infarcted tissue in the ipsilateral hemisphere from the total volume of the contralateral hemisphere. Infarct volume is quantified using Image J software[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.9600 mL 14.7999 mL 29.5998 mL 73.9995 mL
5 mM 0.5920 mL 2.9600 mL 5.9200 mL 14.7999 mL
10 mM 0.2960 mL 1.4800 mL 2.9600 mL 7.4000 mL
15 mM 0.1973 mL 0.9867 mL 1.9733 mL 4.9333 mL
20 mM 0.1480 mL 0.7400 mL 1.4800 mL 3.7000 mL
25 mM 0.1184 mL 0.5920 mL 1.1840 mL 2.9600 mL
30 mM 0.0987 mL 0.4933 mL 0.9867 mL 2.4667 mL
40 mM 0.0740 mL 0.3700 mL 0.7400 mL 1.8500 mL
DMSO 50 mM 0.0592 mL 0.2960 mL 0.5920 mL 1.4800 mL
60 mM 0.0493 mL 0.2467 mL 0.4933 mL 1.2333 mL
80 mM 0.0370 mL 0.1850 mL 0.3700 mL 0.9250 mL
100 mM 0.0296 mL 0.1480 mL 0.2960 mL 0.7400 mL

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Isoxsuprine hydrochloride
目录号:
HY-B1270
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