2. Stem Cell/Wnt
  3. Wnt β-catenin
  4. KY1220

KY1220作用于 Wnt/β-catenin 靶点，使β-catenin 和 Ras不稳定。在HEK293报告细胞中的IC50 值为2.1 μM。

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KY1220 Chemical Structure

KY1220 Chemical Structure

CAS No. : 292168-79-7

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥1380
In-stock
2 mg ￥800
In-stock
5 mg ￥1500
In-stock
10 mg ￥1900
In-stock
50 mg ￥4800
In-stock
100 mg ￥6800
In-stock
200 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

Customer Review

KY1220 相关抗体

Top Publications Citing Use of Products

    KY1220 purchased from MCE. Usage Cited in: Cancer Lett. 2018 Sep 28;432:121-131.  [Abstract]

    The capability of ASPP2 in regulating epithelial–mesenchymal transition (EMT) is completely abolished by specific Wnt/β-catenin inhibitor KY1220.

    KY1220 purchased from MCE. Usage Cited in: Life Sci. 2018 Nov 1;212:129-137.  [Abstract]

    KY1220 reverses the increasing expression of β-Catenin after upregulating Cry1 (P<0.05).

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    KY1220 is a compound that destabilizes both β-catenin and Ras, via targeting the Wnt/β-catenin pathway; with an IC50 of 2.1 μM in HEK293 reporter cells.

    IC50 & Target

    IC50: 2.1 μM (HEK293 reporter cells)[1]
    体外研究
    (In Vitro)

    KY1220 shows an IC50 of 2.1 μM in HEK293 reporter cells. KY1220 dose dependently decreases Wnt3a-CM-induced TOPflash reporter activation and mRNA expression of Wnt target genes CCND1 and MYC in HEK293 cells. In HEK293 cells, both β-catenin and panRas protein levels are similarly reduced in a dose-dependent manner after treatment with KY1220, whereas the mRNA levels of CTNNB1 (which encodes β-catenin), NRAS, KRAS and HRAS remain unchanged. K-Ras, which has a critical role in progression of CRCs, is also destabilized by KY1220 via polyubiquitin-dependent proteasomal degradation. KY1220 accelerates the degradation rates of both β-catenin and Ras in SW480 cell lines. Ras destabilization by KY1220 consequently inhibits the activities of both ERK and Akt, which are downstream effectors of Ras in SW480 cells harboring a KRAS mutation. The proliferation and transformation of the HCT15, SW480, D-WT and D-MT CRC cells are efficiently inhibited after treatment with KY1220[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    KY1220 相关抗体:
    分子量

    314.32

    Formula

    C14H10N4O3S
    CAS 号
    性状

    固体

    颜色

    Light yellow to yellow

    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    溶解性数据
    In Vitro: 

    DMSO 中的溶解度 : 50 mg/mL (159.07 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 3.1815 mL 15.9074 mL 31.8147 mL
    5 mM 0.6363 mL 3.1815 mL 6.3629 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    纯度 & 产品资料
    参考文献
    Cell Assay
    [1]

    HCT15 or SW480 cells are treated with 25 μM KY1220 or KYA1797K or with control (DMSO) for 72 h. Cell proliferation is measured using the MTT assay. The absorbance of the formazan product is determined at 590 nm every 24 h[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    KY1220 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.1815 mL 15.9074 mL 31.8147 mL 79.5368 mL
    5 mM 0.6363 mL 3.1815 mL 6.3629 mL 15.9074 mL
    10 mM 0.3181 mL 1.5907 mL 3.1815 mL 7.9537 mL
    15 mM 0.2121 mL 1.0605 mL 2.1210 mL 5.3025 mL
    20 mM 0.1591 mL 0.7954 mL 1.5907 mL 3.9768 mL
    25 mM 0.1273 mL 0.6363 mL 1.2726 mL 3.1815 mL
    30 mM 0.1060 mL 0.5302 mL 1.0605 mL 2.6512 mL
    40 mM 0.0795 mL 0.3977 mL 0.7954 mL 1.9884 mL
    50 mM 0.0636 mL 0.3181 mL 0.6363 mL 1.5907 mL
    60 mM 0.0530 mL 0.2651 mL 0.5302 mL 1.3256 mL
    80 mM 0.0398 mL 0.1988 mL 0.3977 mL 0.9942 mL
    100 mM 0.0318 mL 0.1591 mL 0.3181 mL 0.7954 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    KY1220292168-79-7KY 1220KY-1220Wntβ-cateninBeta cateninInhibitorinhibitorinhibit

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    目录号：
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