2. Protein Tyrosine Kinase/RTK
  3. Insulin Receptor
  4. Kaempferitrin

Kaempferitrin  (Synonyms: 山奈苷; Lespedin; Lespenephryl)

目录号: HY-N0628 纯度: 99.94%
COA 产品使用指南

摩尔计算器

Kaempferitrin 是一种天然黄酮苷类化合物，具有缓解疼痛、消炎、抗糖尿病、抗肿瘤和化疗的作用，同时可激活胰岛素 (insulin) 信号传导。

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Kaempferitrin Chemical Structure

Kaempferitrin Chemical Structure

CAS No. : 482-38-2

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥1273
In-stock
1 mg ￥500
In-stock
5 mg ￥1000
In-stock
10 mg ￥1700
In-stock
25 mg ￥3520
In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Customer Review

Kaempferitrin 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 1 篇科研文献

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Kaempferitrin is a natural flavonoid, possesses antinociceptive, anti-inflammatory, anti-diabetic, antitumoral and chemopreventive effects, and activates insulin signaling pathway.

IC50 & Target

Insulin Receptor[1]
体外研究
(In Vitro)

Kaempferitrin activates insulin signaling pathway. Kaempferitrin causes survival rates higher than 90% at 1-20 μM in matured 3T3-L1 adipocyte, and the survival rates decline rapidly at 25 and 50 μM. Kaempferitrin (15 μM) increases insulin receptor beta tyrosine phosphorylation and tyrosine phosphorylation of the insulin receptor substrate 1, and such effects are similar to that of 10 nM insulin. Kaempferitrin (15 μM) also stimulates akt phosphorylation on ser473, and the stimulation can be blocked by a PI3-K inhibitor wortmannin. Kaempferitrin potently exerts the translocation of GLUT4 to the membrane of adipocytes at 15 μM, and this is suppressed by wortmannin. In addition, Kaempferitrin increases the total levels of Glu4 protein in differentiated cells and secreted adiponectin in mature 3T3-L1 adipocytes[1]. Kaempferitrin is cytotoxic to human cancer cells such as HeLa and MDA-MB231 cells, with IC50s of 45 ± 2.6 and 65 ± 2.6 μM, and shows low toxic effects on non-tumorigenic cells. Kaempferitrin (45 μM) induces apoptosis of HeLa cells after treatment for 24 and 48 h, and causes reactive oxygen species (ROS) generation in HeLa cells. Furthermore, Kaempferitrin (45 μM) exerts G1 arrest, causes the expression of proteins associated with intrinsic pathway of apoptosis and activates caspase 3 in HeLa cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Kaempferitrin 相关抗体:
体内研究
(In Vivo)

Kaempferitrin (2.5, 10 and 25 mg/kg, i.p.) markedly suppresses the growth of tumor by 40%, 87% and 97%, and decreases tumor weight by 37%, 81% and 95%, respectively in nu/nu mice bearing HeLa tumor. Kaempferitrin also inhibits cell proliferation and extends life span in mice bearing tumor[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

578.52

Formula

C27H30O14
CAS 号
性状

固体

颜色

Light yellow to yellow

中文名称

山奈苷
结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO 中的溶解度 : 25 mg/mL (43.21 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.7285 mL 8.6427 mL 17.2855 mL
5 mM 0.3457 mL 1.7285 mL 3.4571 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

In Vivo:

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (3.60 mM); 澄清溶液

    此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

    生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
  • 方案 二

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (3.60 mM); 澄清溶液

    此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

    20% SBE-β-CD in Saline 的配制（4°C，储存一周）：2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
请输入您的动物体内配方组成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
计算结果
工作液所需浓度 : mg/mL
储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
连续给药周期超过半月以上，请谨慎选择该方案。
请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
纯度 & 产品资料

纯度: 99.94%

COA (267 KB) HNMR (297 KB) LCMS (97 KB)

产品使用指南 (1538 KB)
参考文献
Cell Assay
[1]

Viability assay is carried out by MTT assay. Preconfluent 3T3-L1 preadipocytes are seeded to reach confluence. Kaempferitrin is added in replacement of insulin immediately after confluence (day 0), and the viability is measured 3 h after addition of the compound. Another cell viability assay is performed at day 8. For kaempferitrin combined with insulin treatment, various concentrations of kaempferitrin are added simultaneously with 0.2 nM insulin from day 0 to day 8. Cells without insulin or kaempferitrin treatment during differentiation (day 0 to day 8) are used as control. Finally, to verify the cell viability in matured 3T3-L1 cells treated with insulin or kaempferitrin for 24-48 h, MTT are incubated for 3 h at the end of the 24 h and 48 h period of compound treatments, and survival rates calculated and compared to that without insulin or kaempferitrin[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[2]

The nu/nu mice are injected subcutaneously in their backs with HeLa cells (1.5 × 106). Four hours after tumor implantation, groups of five mice receive doses of Kaempferitrin between 2.5 to 25 mg/kg, dissolved in 0.1 mL of 0.9% saline solution, cisplatin (CDDP) 1 mg/kg or paclitaxel (PCX) 1 mg/kg injected intraperitoneally daily over a period of 32 days. The animal control group received 0.1 mL of vehicle solution. Tumors are measured using a vernier caliper, and their size in mm3 is calculated Tumor volume = lenght × width × height2. At the end of the experiments, animals are sacrificed and their tumors are excised and weighed[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

Kaempferitrin 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7285 mL 8.6427 mL 17.2855 mL 43.2137 mL
5 mM 0.3457 mL 1.7285 mL 3.4571 mL 8.6427 mL
10 mM 0.1729 mL 0.8643 mL 1.7285 mL 4.3214 mL
15 mM 0.1152 mL 0.5762 mL 1.1524 mL 2.8809 mL
20 mM 0.0864 mL 0.4321 mL 0.8643 mL 2.1607 mL
25 mM 0.0691 mL 0.3457 mL 0.6914 mL 1.7285 mL
30 mM 0.0576 mL 0.2881 mL 0.5762 mL 1.4405 mL
40 mM 0.0432 mL 0.2161 mL 0.4321 mL 1.0803 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Kaempferitrin482-38-2Lespedin Lespenephryl山奈苷Insulin ReceptorInhibitorinhibitorinhibit

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