1. Metabolic Enzyme/Protease
  2. Phosphatase
  3. LB-100

LB-100 是一种蛋白磷酸酶 2A (protein phosphatase 2A) 抑制剂,在 BxPc-3 和 Panc-1 细胞中,IC50 值分别为 0.85 μM 和 3.87 μM。

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LB-100 Chemical Structure

LB-100 Chemical Structure

CAS No. : 1632032-53-1

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规格 价格 是否有货 数量
10 mM * 1 mL in Water ¥1181
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2 mg ¥800
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5 mg ¥1200
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10 mg ¥1980
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50 mg ¥6600
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100 mg ¥8150
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Top Publications Citing Use of Products

    LB-100 purchased from MCE. Usage Cited in: Biol Reprod. 2018 May 1;98(5):644-653.  [Abstract]

    Comparison of SIRT3 expression of PA-treated oocytes cultured with or without 10 μM LB100.
    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    LB-100 is a protein phosphatase 2A (PP2A) inhibitor, with IC50 of 0.85 μM and 3.87 μM in BxPc-3 and Panc-1 cells[1][2][3].

    IC50 & Target

    IC50: 0.85 μM (PP2 in BxPc-3 cell), 3.87 μM (PP2 in Panc-1 cell)

    体外研究
    (In Vitro)

    LB-100 抑制细胞生长,IC50 分别为 2.3 μM (BxPc-3)和 1.7 μM (Panc-1)。在 BxPc-3、Panc-1 和 SW1990 细胞中,LB-100 可使 PP2A 活性降低 30-50%。LB-100 增加细胞内阿霉素的浓度(对照 2.5 倍),并使肿瘤细胞对阿霉素的细胞毒性敏感。LB-100 增加 VEGF 分泌,从而增强 HIF-1α-VEGF 介导的血管生成[1]。LB-100 改变内皮细胞间 ve-钙粘蛋白的完整性。LB-100 的预处理结果使通过 HUVECs 单层的染料增加了近 40%。LB-100 诱导血管内皮细胞细胞旁通透性升高,这可能是 LB-100 增加肿瘤细胞中阿霉素浓度的原因[2]。LB-100 下调 Bcl-2 表达,增强索拉非尼诱导的 HCC 细胞凋亡[3]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    体内研究
    (In Vivo)

    LB-100 (2 mg/kg,腹腔注射) 以时间依赖性的方式降低裸鼠异种移植物和肝脏中 PP2A 的活性。经免疫印迹证实,LB-100 不会改变三种 PP2A 亚基 (PP2A_A、PP2A_B 和 PP2A_C) 在细胞系、异种移植物或肝脏中的表达。阿霉素(1.5 kg/mL,每隔一天) 和 LB-100 (2 mg/kg,每隔一天) 联合用药可显著减缓两种动物的肿瘤生长,缩小肿瘤体积,而单药处理对动物的肿瘤生长无影响[2]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    分子量

    268.31

    Formula

    C13H20N2O4

    CAS 号
    性状

    固体

    颜色

    White to off-white

    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    溶解性数据
    In Vitro: 

    H2O 中的溶解度 : ≥ 48 mg/mL (178.90 mM)

    DMSO 中的溶解度 : 1 mg/mL (3.73 mM; 超声助溶 (<60°C); 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

    * "≥" means soluble, but saturation unknown.

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 3.7270 mL 18.6352 mL 37.2703 mL
    5 mM 0.7454 mL 3.7270 mL 7.4541 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 2 years; -20°C, 1 year。-80°C储存时,请在2年内使用, -20°C储存时,请在1年内使用。

    * 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳,请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度,如有需要,请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: ≥98.0%

    参考文献
    Kinase Assay
    [1]

    Cultured pancreatic cancer cells are treated with IC50 of LB-100 for each cell line or equal volume of vehicle for 2 hours, and PP2A activity assays are then performed using Ser/Thr phosphatase assay kit. Cells are lysed with an ultrasonic cell disruptor, and the PP2A concentration is measured using a Ser/Thr phosphatase assay kit according to the instructions. Assays for each cell line are performed in triplicate.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Assay
    [1]

    Cytotoxicity is conducted by using a Cell Counting Kit-8. Cells are seeded in 96-well plates with a density of 3000 cells per well and are assessed after treatments following the CCK-8 protocol. Relative cytotoxicity is expressed as a percentage of specific controls.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [2]

    BALB/c nude mice are injected subcutaneously in the right flank with 1×106 Huh-7 cells suspended in 200 μL PBS per mouse. After a tumor volume of 100 to 200 mm3 is reached, tumor-bearing mice are randomLy allocated to four groups: control group, doxorubicin/cisplatin group, LB-100 group, and doxorubicin/cisplatin plus LB-100 group. For the doxorubicin plus LB-100 study (n=6 to 8), doxorubicin and LB-100 are injected i.p. at 1.5 and 2 mg/kg, respectively, on alternate days for a total of 16 days. For the cisplatin plus LB-100 study (n=8 to 10), cisplatin and LB-100 are injected at 3 and 2.5 mg/kg, i.p., respectively; cisplatin is injected every 4 days and LB-100 is used every other day for 16 days. Control mice are injected with DMSO (in the doxorubicin plus LB-100 group) or PBS (in the cisplatin plus LB-100 group) on the same schedule as the drug-treated animals. Tumor size is monitored every 3 or 4 days, and is calculated by the formula: tumor volume=length × width × height/2. All mice are sacrificed at day 16, and xenografts are obtained, weighed, and fixed with 10% formaldehyde.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    参考文献

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 2 years; -20°C, 1 year。-80°C储存时,请在2年内使用, -20°C储存时,请在1年内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 3.7270 mL 18.6352 mL 37.2703 mL 93.1758 mL
    H2O 5 mM 0.7454 mL 3.7270 mL 7.4541 mL 18.6352 mL
    10 mM 0.3727 mL 1.8635 mL 3.7270 mL 9.3176 mL
    15 mM 0.2485 mL 1.2423 mL 2.4847 mL 6.2117 mL
    20 mM 0.1864 mL 0.9318 mL 1.8635 mL 4.6588 mL
    25 mM 0.1491 mL 0.7454 mL 1.4908 mL 3.7270 mL
    30 mM 0.1242 mL 0.6212 mL 1.2423 mL 3.1059 mL
    40 mM 0.0932 mL 0.4659 mL 0.9318 mL 2.3294 mL
    50 mM 0.0745 mL 0.3727 mL 0.7454 mL 1.8635 mL
    60 mM 0.0621 mL 0.3106 mL 0.6212 mL 1.5529 mL
    80 mM 0.0466 mL 0.2329 mL 0.4659 mL 1.1647 mL
    100 mM 0.0373 mL 0.1864 mL 0.3727 mL 0.9318 mL

    * 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    产品名称:
    LB-100
    目录号:
    HY-18597
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