1. GPCR/G Protein
  2. Angiotensin Receptor
  3. Losartan potassium

Losartan potassium  (Synonyms: 氯沙坦钾; DuP-753 potassium)

目录号: HY-17512A 纯度: 99.91%
COA 产品使用指南

Losartan potassium (DuP-753 potassium) 是血管紧张素 II 受体 1 型 (AT1)拮抗剂,与血管紧张素 II 与 AT1 的结合竞争,IC50 为 20 nM。

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Losartan potassium Chemical Structure

Losartan potassium Chemical Structure

CAS No. : 124750-99-8

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥500
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500 mg ¥400
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1 g ¥500
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5 g ¥1200
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50 g   询价  

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Other Forms of Losartan potassium:

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Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 28 篇科研文献

WB

    Losartan potassium purchased from MCE. Usage Cited in: FASEB J. 2018 Sep;32(9):5051-5062.  [Abstract]

    HUVECs are starved for 12 h, treated with 10 mg/mL (9.56 μM) of AngII with or without Losartan or PD123319 for 12 h, and are subsequently used for Western blot analysis.

    查看 Angiotensin Receptor 亚型特异性产品:

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Losartan potassium (DuP-753 potassium) is an angiotensin II receptor type 1 (AT1) antagonist, competing with the binding of angiotensin II to AT1 with an IC50 of 20 nM.

    IC50 & Target

    AT1 Receptor

     

    体外研究
    (In Vitro)

    Losartan potassium 竞争血管紧张素 II 与 AT1 受体的结合。抑制 50% 血管紧张素 II 结合的浓度 (IC50) 为 20 nM[1]。Losartan potassium (40 μM) 影响 ISC 但阻止 ANGII 对 ISC 的影响[2]。Losartan potassium 显著降低子宫内膜癌细胞中 Ang II 介导的细胞增殖。与单独使用每种药物相比,Losartan potassium 和抗 miR-155 的组合具有显著更强的抗增殖作用[3]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    体内研究
    (In Vivo)

    Losartan potassium (0.6 g/L,po) 处理的 Fbn1C1039G/+ 小鼠显示相对于安慰剂处理的 Fbn1C1039G 远端空域口径减少/+ 。滴定 Losartan potassium 和普萘洛尔的剂量以达到可比较的血液动力学效果。pSmad2 核染色分析表明,Losartan potassium 拮抗 Fbn1C1039G/+ 小鼠主动脉壁中的 TGF-β 信号传导。Losartan potassium 可以改善肺部的疾病表现,这一事件与改善的血流动力学似乎无关[4]。Losartan potassium (10 mg/kg,动脉内注射) 使血液血管紧张素水平增加四到六倍。Losartan potassium (10 mg/kg,ip) 使血浆肾素水平增加 100 倍;血浆血管紧张素原水平降至对照的 24%;和血浆醛固酮水平没有变化[5]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Clinical Trial
    分子量

    461.00

    Formula

    C22H22ClKN6O

    CAS 号
    性状

    固体

    颜色

    White to off-white

    中文名称

    氯沙坦钾

    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 1 years; -20°C, 6 months (sealed storage, away from moisture)

    溶解性数据
    In Vitro: 

    DMSO 中的溶解度 : ≥ 110 mg/mL (238.61 mM; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

    H2O 中的溶解度 : 50 mg/mL (108.46 mM; 超声助溶)

    * "≥" means soluble, but saturation unknown.

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 2.1692 mL 10.8460 mL 21.6920 mL
    5 mM 0.4338 mL 2.1692 mL 4.3384 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 1 years; -20°C, 6 months (sealed storage, away from moisture)。-80°C储存时,请在1年内使用, -20°C储存时,请在6个月内使用。

    * 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    In Vivo:

    以下溶解方案,请直接配置工作液。建议现用现配,在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比; 如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂: PBS

      Solubility: 100 mg/mL (216.92 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳,请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度,如有需要,请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.91%

    参考文献
    Cell Assay
    [3]

    An MTT assay is used to measure cell proliferation and viability. For the assay, 5000 cells in 200 μL media per well are seeded in a 96 well plate. After overnight incubation to allow for cell attachment, the medium is removed by suction. MTT at 1 mg/mL concentration in serum-free medium is added and then incubated for 4 h at 37°C. After removal of MTT solution, 100 μL of DMSO is added to dissolve formazan crystals. Absorbance at 570 nm and at 600 nm as a reference is then measured using a microplate reader. The difference in absorbance is thus relative to the extent of cell survival.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [4]

    Female Fbn1C1039G/+ mice undergo timed matings with wild-type male mice. At 14.5d post-coitum, pregnant female Fbn1C1039G/+ mice are treated with oral losartan (0.6 g/L in drinking water; n=10), propranolol (0.5 g/L; n=6) or placebo (n=12). Therapy is continued throughout lactation and after weaning until 10 months of age. Mice are sacrificed and examined using the techniques described above. Propranolol is used for comparison with losartan because β-adrenergic receptor blockade is the current albeit controversial standard of care to modulate abnormal growth of the aortic root in MFS. Beginning at 7 weeks of age, wild-type and Fbn1C1039G/+ mice are treated with oral losartan (0.6 g/L in drinking water; n=5), propranolol (0.5 g/L; n=7) or placebo (n=10). Mice are continued on oral therapy for 6 months and then sacrificed.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    参考文献

    Losartan potassium 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 1 years; -20°C, 6 months (sealed storage, away from moisture)。-80°C储存时,请在1年内使用, -20°C储存时,请在6个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 2.1692 mL 10.8460 mL 21.6920 mL 54.2299 mL
    5 mM 0.4338 mL 2.1692 mL 4.3384 mL 10.8460 mL
    10 mM 0.2169 mL 1.0846 mL 2.1692 mL 5.4230 mL
    15 mM 0.1446 mL 0.7231 mL 1.4461 mL 3.6153 mL
    20 mM 0.1085 mL 0.5423 mL 1.0846 mL 2.7115 mL
    25 mM 0.0868 mL 0.4338 mL 0.8677 mL 2.1692 mL
    30 mM 0.0723 mL 0.3615 mL 0.7231 mL 1.8077 mL
    40 mM 0.0542 mL 0.2711 mL 0.5423 mL 1.3557 mL
    50 mM 0.0434 mL 0.2169 mL 0.4338 mL 1.0846 mL
    60 mM 0.0362 mL 0.1808 mL 0.3615 mL 0.9038 mL
    80 mM 0.0271 mL 0.1356 mL 0.2711 mL 0.6779 mL
    100 mM 0.0217 mL 0.1085 mL 0.2169 mL 0.5423 mL

    * 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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