1. Apoptosis Autophagy
  2. c-Myc Autophagy
  3. ML327

ML327 是 MYC 的阻断剂,也可以去阻遏 E-钙粘蛋白转录和逆转上皮-间质转化 (EMT)。

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ML327 Chemical Structure

ML327 Chemical Structure

CAS No. : 1883510-31-3

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥1540
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1 mg ¥560
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5 mg ¥1400
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10 mg ¥2200
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25 mg ¥4500
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50 mg ¥7200
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100 mg   询价  
200 mg   询价  

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Top Publications Citing Use of Products
  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

ML327 is a blocker of MYC which can also de-repress E-cadherin transcription and reverse Epithelial-to-Mesenchymal Transition (EMT).

IC50 & Target

MYC[1]

体外研究
(In Vitro)

用 ML327 处理可诱导神经母细胞瘤细胞的细长形态。用 ML327 处理的 BE (2)-C 细胞显示 G1 细胞周期停滞,S 期细胞群一致减少,而亚 G0 细胞群显著增加。ML327 在所有七种神经母细胞瘤细胞系中诱导 CDH1 的表达,CDH1 mRNA 表达诱导 50 到 1,400 倍。ML327 在所有测试的神经母细胞瘤细胞系中阻断致癌转录因子 MYC 家族的表达。免疫印迹时间进程表明在使用 ML327 (10 μM) 处理后 2 小时内 N-MYC 表达的早期抑制。p53 水平也被 ML327 处理抑制。ML327 预处理的细胞在基于四唑盐的 (p<0.0001) 和贴壁 2D 集落形成 (41 对 400;p<0.0001)[1]中均表现出降低的增殖潜力。在这些体外测定中,ML327 通过 Matrigel 减少 SW620inv 细胞侵袭约 60%,并将 H520 细胞侵袭减少约 30%。在通过 TGF-β1 处理诱导上皮-间质转化 (EMT) 的 NMuMG 细胞中,ML327 部分恢复质膜上的 E-钙粘蛋白表达[2]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

ML327 处理在两周处理期间显著减少了三倍的肿瘤体积 (p=0.02)。ML327 处理的小鼠的肿瘤外植体重量大约小三倍 (p=0.01)。用 ML327 处理的小鼠比载体处理的小鼠多失去 12% 的体重。ML327 处理导致 MYCN 表达降低两倍,证实 ML327 抑制异种移植物 MYCN 表达 (p=0.0035)[1]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

366.37

Formula

C19H18N4O4

CAS 号
性状

固体

颜色

White to off-white

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
溶解性数据
In Vitro: 

DMSO 中的溶解度 : 32 mg/mL (87.34 mM; 超声加热助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.7295 mL 13.6474 mL 27.2948 mL
5 mM 0.5459 mL 2.7295 mL 5.4590 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 2 years; -20°C, 1 year。-80°C储存时,请在2年内使用, -20°C储存时,请在1年内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 98.06%

参考文献
Cell Assay
[1]

Cells are seeded onto 96-well plates at equivalent density (3,000 to 10,000 depending upon cell line), permitted to attach overnight, and treated with either ML327 (10 μM) or vehicle. Daily absorbance measurements (450 nm) using the cell counting kit are obtained. For estimation of IC50 values, cells are plated at equal density, permitted to attach, and baseline absorbance is obtained using cell counting kit. Cells are then treated with varying doses of ML327 (0.1 to 30 μM) and cell viability is measured 72 h after treatment[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Male athymic nude mice (4 to 6 weeks old) are maintained as described. BE(2)-C cells xenografts are established as previously described. Briefly, 1×106 cells/100 µL of HBSS are injected subcutaneously into flanks using a 26-gauge needle (n=10 per group). Mice are monitored daily for xenograft formation and assessed by measuring the two greatest perpendicular tumor diameter with venier calipers. Xenograft volumes are estimated using the following formula [(length×width2)/2]. Once tumors reach 75 to 100 mm3, mice are randomized to receive either 50 mg/kg of ML327 or control vehicle (70% polyethylene glycol) via intraperitoneal injection twice daily for 14d. Weight and tumor volume are recorded daily. After completion of two weeks of treatment, mice are euthanized and tumors are excised, weighed, and RNA is isolated[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

ML327 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 2 years; -20°C, 1 year。-80°C储存时,请在2年内使用, -20°C储存时,请在1年内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7295 mL 13.6474 mL 27.2948 mL 68.2370 mL
5 mM 0.5459 mL 2.7295 mL 5.4590 mL 13.6474 mL
10 mM 0.2729 mL 1.3647 mL 2.7295 mL 6.8237 mL
15 mM 0.1820 mL 0.9098 mL 1.8197 mL 4.5491 mL
20 mM 0.1365 mL 0.6824 mL 1.3647 mL 3.4119 mL
25 mM 0.1092 mL 0.5459 mL 1.0918 mL 2.7295 mL
30 mM 0.0910 mL 0.4549 mL 0.9098 mL 2.2746 mL
40 mM 0.0682 mL 0.3412 mL 0.6824 mL 1.7059 mL
50 mM 0.0546 mL 0.2729 mL 0.5459 mL 1.3647 mL
60 mM 0.0455 mL 0.2275 mL 0.4549 mL 1.1373 mL
80 mM 0.0341 mL 0.1706 mL 0.3412 mL 0.8530 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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ML327
目录号:
HY-103038
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