1. GPCR/G Protein
  2. Neuropeptide Y Receptor

Pancreatic Polypeptide, rat (Synonyms: Rat pancreatic polypeptide)

目录号: HY-P1532
产品使用指南

Pancreatic Polypeptide, rat 是神经肽 (NPY) 受体激动剂,对 NPYR4 有很高的亲和性。

MCE 的所有产品仅用作科学研究,我们不为任何个人用途提供产品和服务

Custom Peptide Synthesis

Pancreatic Polypeptide, rat Chemical Structure

Pancreatic Polypeptide, rat Chemical Structure

CAS No. : 90419-12-8

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

Size Price Stock
500 μg ¥3120 Get quote
1 mg ¥5280 Get quote
5 mg ¥15600 Get quote

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • 生物活性

  • 实验参考方法

  • 技术信息

  • 参考文献

Description

Pancreatic Polypeptide, rat is an agonist of NPY receptor, with high affinity at NPYR4.

IC50 & Target

NPYR4[1]

In Vitro

Pancreatic Polypeptide, rat is an agonist of NPY receptor, with high affinity at NPYR4. Pancreatic Polypeptide (1 μM) does not alter proliferation in BRIN BD11 or 1.1B4 beta-cells, but reverses the decreased cell viability in BRIN BD11 cells induced by streptozotocin. Pancreatic Polypeptide (0.1 nM-1 μM) shows no effect on insulin secretion from isolated mouse islets, and does not affect the membrane potential and (Ca2+)i levels in BRIN BD11 cells at 1 μM[1].

In Vivo

Pancreatic Polypeptide (25 nmol/kg bw, i.p.) reduces glucose-stimulated insulin concentrations but shows no effect on acute feeding behaviour in overnight fasted mice[1].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 0.2273 mL 1.1367 mL 2.2733 mL
5 mM 0.0455 mL 0.2273 mL 0.4547 mL
10 mM 0.0227 mL 0.1137 mL 0.2273 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay
[1]

To assess the effects of NPY and Pancreatic Polypeptide on rodent BRIN-BD11 and human 1.1B4 beta-cell proliferation, cells are seeded at a density of 150,000 cells per well and cultured overnight in the presence of NPY or Pancreatic Polypeptide (1 μM), and compared to positive control GLP-1 (1 μM). Cells are washed with PBS and fixed using 4% paraformaldehyde. After antigen retrieval with citrate buffer at 95°C for 20 min, tissue is blocked using 2% BSA for 45 min. The slides are then incubated with rabbit anti-Ki-67 primary antibody, and subsequently with Alexa Fluor® 594 secondary antibody. Slides are viewed using fluorescent microscope and photographed by DP70 camera adapter system. Proliferation frequency is determined in a blinded fashion and expressed as % of total cells analysed. Approximately 150 cells per replicate are analysed. For analysis of ability of NPY and Pancreatic Polypeptide to protect against streptozotocin-induced DNA damage, BRIN-BD11 and 1.1B4 cells are seeded. Cells are then exposed to streptozotocin (5 mM) in the presence or absence of NPY or Pancreatic Polypeptide (1 μM) for 2 h, with GLP-1 (1 μM) as positive control. Cells are then harvested and a comet assay is performed. Resulting gels are stained using DAPI (4′,6-diamidino-2-phenylindole) (100 μg/mL) and slides are viewed under appropriate filter. Comet score software is used for the analysis of % tail DNA (100 cells per gel) and olive tail moment[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Pancreatic Polypeptide is formulated in 0.9% (w/v) NaCl[1].

Mice[1]
Plasma glucose and insulin responses are evaluated after intraperitoneal (i.p.) injection of glucose alone (18 mmol/kg body weight) or in combination with test peptides (Pancreatic Polypeptide, etc.; 25 nmol/kg body weight) in overnight (18 h) fasted C57BL/6 mice. In a second series of experiments, 18 h fasted mice are used to assess the effects of respective test peptides on food intake. Mice receive an i.p. injection of saline alone (0.9% (w/v) NaCl) or in combination with test peptides (25 nmol/kg body weight) and food intake measured at 30 min intervals for 180 min. A dose of 25 nmol/kg is chosen with other NPYR modulators on glucose homeostasis, insulin secretion and feeding at this dose[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

4398.87

Formula

C₁₉₅H₂₉₈N₅₈O₅₇S

CAS No.

90419-12-8

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

H2O

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
Pancreatic Polypeptide, rat
目录号:
HY-P1532
需求量: