Parasin I (Synonyms: 鲶鱼抗菌肽 I)

目录号: HY-P0324: FAQs
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Parasin I 是一种由 19 个氨基酸残基组成的多肽，源于从鲶鱼皮肤分离到的组蛋白 H2A，具有抗菌的作用。

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Parasin I Chemical Structure

CAS No. : 219552-69-9

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生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性	Parasin I is a 19-amino acid histone H2A-derived peptide isolated from the skin of the catfish, and shows antimicrobial activity.
体外研究 (In Vitro)	Parasin I with comparable antimicrobial activities localized to the cell membrane and subsequently permeabilized the outer and cytoplasmic membranes. Parasin I and its active analogs show strong cytoplasmic membrane permeabilizing activity^[1]. Codon optimized parasin I fused with human lysozyme is expressed in Pichia pastoris, and has potent antibiotic activity^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Parasin I 相关抗体:
分子量	2000.31
Formula	C₈₂H₁₅₄N₃₄O₂₄
CAS 号	219552-69-9
Sequence	Lys-Gly-Arg-Gly-Lys-Gln-Gly-Gly-Lys-Val-Arg-Ala-Lys-Ala-Lys-Thr-Arg-Ser-Ser
Sequence Shortening	KGRGKQGGKVRAKAKTRSS
中文名称	鲶鱼抗菌肽 I
结构分类	Others
初始来源	动物 Parasilurus asotus
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料	Data Sheet (520 KB) 产品使用指南 (1538 KB)
参考文献	[1]. Koo YS, et al. Structure-activity relations of parasin I, a histone H2A-derived antimicrobial peptide. Peptides. 2008 Jul;29(7):1102-8. [Content Brief] [2]. Zhao H, et al. Characterization of bioactive recombinant antimicrobial peptide parasin I fused with human lysozyme expressed in the yeast Pichia pastoris system. Enzyme Microb Technol. 2015 Sep;77:61-7. [Content Brief]

Cell Assay ^[1]	The antimicrobial activity of each peptide is determined using the broth microdilution assay. Briefly, single colonies of bacteria and fungi are inoculated into 3% trypticase soy broth (TSB) and Saboraud's medium, respectively, and cultured overnight at 37 and 30°C, respectively. Aliquots of each culture are transferred to 50 mL of fresh medium and incubated for an additional 3-6 h to obtain midlogarithmic phase cells. The cells are then washed and resuspended in 10 mM sodium phosphate buffer (NAPB), pH 7.4. The relationship between absorbance at 620 nm and colony-forming units (cfus) is determined for each microorganism by spreading serial dilutions of the cell suspension onto TSB or Saboraud agar plates. The cell suspension is diluted to 5×10⁵ cfu/mL with 10 mM NAPB. Each well of 96- well propylene microtiter plates is filled with 90 mL of the diluted suspension and 10 mL of serially diluted peptide samples. After incubation for 3 h, fresh medium is added to the mixture and incubated at 37°C (bacteria) or 30°C (fungi) for an additional 16 h. The inhibition of growth is determined by measuring absorbance at 620 nm with a Model 550 Microplate Reader. The lowest concentration of peptide that completely inhibits growth is defined as the ‘minimal inhibitory concentration’ (MIC). The MICs are the average values obtained in triplicates in three independent experiments. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Koo YS, et al. Structure-activity relations of parasin I, a histone H2A-derived antimicrobial peptide. Peptides. 2008 Jul;29(7):1102-8. [Content Brief] [2]. Zhao H, et al. Characterization of bioactive recombinant antimicrobial peptide parasin I fused with human lysozyme expressed in the yeast Pichia pastoris system. Enzyme Microb Technol. 2015 Sep;77:61-7. [Content Brief]

Cell Assay
^[1]

The antimicrobial activity of each peptide is determined using the broth microdilution assay. Briefly, single colonies of bacteria and fungi are inoculated into 3% trypticase soy broth (TSB) and Saboraud's medium, respectively, and cultured overnight at 37 and 30°C, respectively. Aliquots of each culture are transferred to 50 mL of fresh medium and incubated for an additional 3-6 h to obtain midlogarithmic phase cells. The cells are then washed and resuspended in 10 mM sodium phosphate buffer (NAPB), pH 7.4. The relationship between absorbance at 620 nm and colony-forming units (cfus) is determined for each microorganism by spreading serial dilutions of the cell suspension onto TSB or Saboraud agar plates. The cell suspension is diluted to 5×10⁵ cfu/mL with 10 mM NAPB. Each well of 96- well propylene microtiter plates is filled with 90 mL of the diluted suspension and 10 mL of serially diluted peptide samples. After incubation for 3 h, fresh medium is added to the mixture and incubated at 37°C (bacteria) or 30°C (fungi) for an additional 16 h. The inhibition of growth is determined by measuring absorbance at 620 nm with a Model 550 Microplate Reader. The lowest concentration of peptide that completely inhibits growth is defined as the ‘minimal inhibitory concentration’ (MIC). The MICs are the average values obtained in triplicates in three independent experiments.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Koo YS, et al. Structure-activity relations of parasin I, a histone H2A-derived antimicrobial peptide. Peptides. 2008 Jul;29(7):1102-8. [Content Brief]

[2]. Zhao H, et al. Characterization of bioactive recombinant antimicrobial peptide parasin I fused with human lysozyme expressed in the yeast Pichia pastoris system. Enzyme Microb Technol. 2015 Sep;77:61-7. [Content Brief]

Parasin I 相关分类

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量		浓度		体积		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)	×	体积 _(start)	=	浓度 _(final)	×	体积 _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Parasin I219552-69-9鲶鱼抗菌肽 IBacterialInhibitorinhibitorinhibit

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Parasin I (Synonyms: 鲶鱼抗菌肽 I)

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Other Forms of Parasin I:

Parasin I 相关产品

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实验参考方法

纯度 & 产品资料

参考文献

Parasin I 相关分类

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Parasin I (Synonyms: 鲶鱼抗菌肽 I)

Customer Review

Other Forms of Parasin I:

Parasin I 相关抗体

Parasin I 相关产品

•同靶点产品:

•同靶点蛋白产品:

生物活性

实验参考方法

纯度 & 产品资料

参考文献

Parasin I 相关抗体:

Parasin I 相关分类

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The molarity calculator equation

The dilution calculator equation

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