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Procion Blue HB  (Synonyms: 蓝色染料; Reactive Blue 2)

目录号: HY-D0965 纯度: ≥95.0%
COA 产品使用指南

Procion Blue HB (Reactive Blue 2)是嘌呤能拮抗剂。

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Procion Blue HB Chemical Structure

Procion Blue HB Chemical Structure

CAS No. : 12236-82-7

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample (0.1 - 0.2 mg)   Apply now  
10 mM * 1 mL in DMSO ¥550
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5 mg ¥312
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10 mg ¥500
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50 mg ¥1500
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100 mg ¥1900
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500 mg   询价  

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Top Publications Citing Use of Products
  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Procion Blue HB (Reactive Blue 2) is a purinergic antagonist.

体外研究
(In Vitro)

Reactive Blue 2 is used as an ATP receptor antagonist and induces Ca2+ oscillations in HeLa cells. Reactive Blue 2 enhances a Ca2+ response to histamine that is linked to the PLC cascade. Reactive Blue 2 may activate the PLC cascade in an extracellular Ca2+-dependent manner and induce Ca2+ oscillations[1]. The application of Reactive Blue 2 increases K+ secretion in a dose-dependent manner, and this increase is characterized as a peak followed by a partial relaxation to a steady-state. Reactive Blue 2 has antagonistic activities at P2Y4, and the antagonist potency at P2Y4 paralleled the potency of K+ secretion[2].The anthraquinone dye reactive blue 2 is found to be a potent inhibitor of a protein kinase isolated and purified from thylakoids. The mode of inhibition is noncompetitive, with a Ki of 8 μM for the membrane-bound kinase, and 6 microM for the purified kinase. The inhibitor does not modify the substrate preference of the endogenous kinase and could be removed from the membrane by washing[3]. Reactive blue 2 selectively inhibits responses mediated via the P2ypurinoceptor, at least within a limited concentration range. In preparations where the tone has been raised with noradrenaline, ATP and 2-methylthio ATP, but not α,β-methylene ATP, produce relaxations of the vessel. These relaxations are inhibited in the presence of reactive blue 2[4]. Reactive blue 2, at concentrations of 0.3-10 μM blocks the ATP-induced oscillation in a concentration-dependent manner[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

774.16

Formula

C29H20ClN7O11S3

CAS 号
性状

固体

颜色

Light brown to black

中文名称

蓝色染料;普施安蓝 HB;活性蓝 2;汽巴蓝 F3GA

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO 中的溶解度 : ≥ 110 mg/mL (142.09 mM; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

H2O 中的溶解度 : 10 mg/mL (12.92 mM; 超声助溶)

Ethanol 中的溶解度 : < 1 mg/mL (insoluble)

* "≥" means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.2917 mL 6.4586 mL 12.9172 mL
5 mM 0.2583 mL 1.2917 mL 2.5834 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献

Procion Blue HB 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.2917 mL 6.4586 mL 12.9172 mL 32.2931 mL
5 mM 0.2583 mL 1.2917 mL 2.5834 mL 6.4586 mL
10 mM 0.1292 mL 0.6459 mL 1.2917 mL 3.2293 mL
DMSO 15 mM 0.0861 mL 0.4306 mL 0.8611 mL 2.1529 mL
20 mM 0.0646 mL 0.3229 mL 0.6459 mL 1.6147 mL
25 mM 0.0517 mL 0.2583 mL 0.5167 mL 1.2917 mL
30 mM 0.0431 mL 0.2153 mL 0.4306 mL 1.0764 mL
40 mM 0.0323 mL 0.1615 mL 0.3229 mL 0.8073 mL
50 mM 0.0258 mL 0.1292 mL 0.2583 mL 0.6459 mL
60 mM 0.0215 mL 0.1076 mL 0.2153 mL 0.5382 mL
80 mM 0.0161 mL 0.0807 mL 0.1615 mL 0.4037 mL
100 mM 0.0129 mL 0.0646 mL 0.1292 mL 0.3229 mL

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Procion Blue HB
目录号:
HY-D0965
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