2. Cell Cycle/DNA Damage Anti-infection
  3. Nucleoside Antimetabolite/Analog HIV
  4. Pseudothymidine

Pseudothymidine  (Synonyms: 5-Methyl-2'-Deoxypseudouridin)

目录号: HY-101969 纯度: 99.44%
COA 产品使用指南

摩尔计算器

Pseudothymidine 是胸苷的 C-核苷 (C-nucleosid) 类似物。

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Pseudothymidine Chemical Structure

Pseudothymidine Chemical Structure

CAS No. : 65358-15-8

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥5577
In-stock
1 mg ￥1690
In-stock
5 mg ￥5070
In-stock
10 mg ￥8100
In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Customer Review

Pseudothymidine 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Pseudothymidine is a C-nucleoside analog of thymidine.

体外研究
(In Vitro)

Pseudothymidine is a C-nucleoside analog of thymidine[1]. The calculated ΔΔG°50/mod is -0.5 kcal/mol, with a ΔTm/mod of 0.82°C. For the duplexes containing nine dA-T/ψT pairs, the ΔTm/mod is -0.9°C and a ΔΔG°50/mod is +1.1 kcal/mol. The modification of the duplex containing 12 consecutive dA-T/ψT base pairs produces a ΔTm/mod of -0.9°C and a ΔΔG°50/mod of +1.2 kcal/mol[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Pseudothymidine 相关抗体:
分子量

242.23

Formula

C10H14N2O5
CAS 号
性状

固体

颜色

Light yellow to yellow

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO 中的溶解度 : 61.17 mg/mL (252.53 mM; 超声加热助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 4.1283 mL 20.6415 mL 41.2831 mL
5 mM 0.8257 mL 4.1283 mL 8.2566 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献
Kinase Assay

Thermal DNA duplex denaturation studies are performed with templates containing up to twelve consecutive dA residues that are paired with its complement template containing consecutive T or Pseudothymidine (ψT) residues. Experiments are performed in a buffer (45 mM NaCl, 45 mM sodium citrate, pH 8.1, final vol. 1.5 mL) containing template and its complement (1.5 μM of each). Absorbance (260 nm) is monitored over a range of 25.0 to 90.0°C with a change in temperature of 0.5°C/min for five heating cycles. The initial heating cycle is discarded and the Tm is determined by averaging the temperatures of the remaining four cycles. The ΔTm between similar duplexes is calculated by subtracting the Tm of the duplex containing standard bases from the Tm of the duplex containing C-glycosides (including Pseudothymidine)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

Pseudothymidine 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.1283 mL 20.6415 mL 41.2831 mL 103.2077 mL
5 mM 0.8257 mL 4.1283 mL 8.2566 mL 20.6415 mL
10 mM 0.4128 mL 2.0642 mL 4.1283 mL 10.3208 mL
15 mM 0.2752 mL 1.3761 mL 2.7522 mL 6.8805 mL
20 mM 0.2064 mL 1.0321 mL 2.0642 mL 5.1604 mL
25 mM 0.1651 mL 0.8257 mL 1.6513 mL 4.1283 mL
30 mM 0.1376 mL 0.6881 mL 1.3761 mL 3.4403 mL
40 mM 0.1032 mL 0.5160 mL 1.0321 mL 2.5802 mL
50 mM 0.0826 mL 0.4128 mL 0.8257 mL 2.0642 mL
60 mM 0.0688 mL 0.3440 mL 0.6881 mL 1.7201 mL
80 mM 0.0516 mL 0.2580 mL 0.5160 mL 1.2901 mL
100 mM 0.0413 mL 0.2064 mL 0.4128 mL 1.0321 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pseudothymidine65358-15-85-Methyl-2'-DeoxypseudouridinNucleoside Antimetabolite/AnalogHIVHuman immunodeficiency virusInhibitorinhibitorinhibit

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