2. NF-κB
  3. NF-κB
  4. Pyrrolidinedithiocarbamate ammonium

Pyrrolidinedithiocarbamate ammonium  (Synonyms: 吡咯烷二硫代氨基甲酸铵; Ammonium pyrrolidinedithiocarbamate; PDTC ammonium; APDC)

目录号: HY-18738 纯度: 98.57%
COA 产品使用指南

摩尔计算器

Pyrrolidinedithiocarbamate ammonium (Ammonium pyrrolidinedithiocarbamate) 是选择性和具有血脑屏障通透性的 NF-κB 抑制剂。

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Pyrrolidinedithiocarbamate ammonium Chemical Structure

Pyrrolidinedithiocarbamate ammonium Chemical Structure

CAS No. : 5108-96-3

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50 mg ￥300
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100 mg ￥500
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Pyrrolidinedithiocarbamate ammonium 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 95 篇科研文献

WB
RT-PCR
Proliferation Assay
Cell Viability Assay

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Acta Pharmacol Sin. 2023 Feb 24.  [Abstract]

    Pyrrolidinedithiocarbamate ammonium (PDTC; 100 μM; 24 h) decreases viability of HepG2 and Huh7 cells on the basis of aspirin.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Acta Pharm Sin B. 2022 Sep;12(9):3618-3638.

    SCT2 depletion-senescent LX2 cells are treated with rIL-1α protein and addressed in NF-κB inhibitor PDTC (100 μM), and the analysis of mRNAs of proinflammatory SASP IL1A, IL1B, IL6 and IL8 by qPCR.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Clin Transl Med. 2022 Jun;12(6):e850.  [Abstract]

    PEMs are treated with the NF-κB inhibitor PDTC (100 μM) for 1h followed by BSA or UGRP1 (0.5 μg/mL) treatment for 6 h, then stimulated by Pam3CSK4 for 0.5 h, p65 in the whole cell lysate and nucleus is analysed by immunoblot.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Clin Transl Med. 2022 Jun;12(6):e850.  [Abstract]

    PEMs are treated with the NF-κB inhibitor PDTC (100 μM) for 1 h followed by BSA or UGRP1 (0.5 μg/mL) treatment and Pam3CSK4 stimulation for 6 h to measure Il6 mRNA by qRT‐PCR .

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: JACC Basic Transl Sci. 2022 Aug 3;7(8):820-840.

    To pharmacologically inhibit NFκB, mice are given Pyrrolidinedithiocarbamate ammonium (PDTC; 100 mg/kg).

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Int J Biol Macromol. 2022 Oct 21;S0141-8130(22)02401-1.

    TLR4 and downstream pathways are subjected to relevant western blotting. After the treatment with TJ-M2010, TRAF-stop, and PDTC (5 μM) for 1 h, DCs are stimulated with 10 μg/mL CARP2 for 0, 45, and 90 min.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Oncogene. 2022 Mar;41(13):1866-1881.  [Abstract]

    The Cxcl10 mRNA expression in B16F0 cells treated with IFNα (1 000 U/ml) for 12 hours, in presence of PDTC (10 μM) or BAY 11-7082 (5 μM).

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Theranostics. 2021 Jan 19;11(7):3392-3416.  [Abstract]

    Treatment with NF-κB (PDTC) inhibitor in both PC9 and H1975 cells treated with different concentrations of specific inhibitor for 72 h and Western blotting is performed.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Redox Biol. 2021 Oct;46:102098.

    Several specific pharmacological inhibitors, including Pyrrolidine dithiocarbamate (PDTC, 100 μM), Wortmannin (Wort), Stattic, PD98059 and Bisindolylmaleimide I (Bis), are pre-administered to the cardiomyocytes in the HG + Pae group and then the expression of Opa1 is quantified.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: EMBO Rep. 2021 Jul 5;22(7):e51678.  [Abstract]

    qPCR analysis of Mmps including Mmp9, Mmp10, and Mmp12 from THP‐1 cells stimulated with AG (1 μg/mL) for 24 h in the absence or presence of PDTC at the concentration of 10 μM.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Int J Biol Sci. 2019 Jan 24;15(3):688-700.  [Abstract]

    T24 cells are treated with PDTC at various concentrations for 24-h, and cell viability and IC50 values are measured using MTS assays.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Cell Death Dis. 2019 Feb 8;10(2):113.   [Abstract]

    RAW cells are pre-treated with rMMP-9 and/or PDTC (50 μM) 1 h followed by 18 h of PBS/LPS challenged, and then expressions of representative M1 marker genes.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Oncoimmunology. 2018 Aug 23;7(11):e1461303.  [Abstract]

    The expression of Oct4 in A549 and H460 cells treated with or without rhIL-17A and these molecular inhibitors (NF-kB inhibitor: PDAC, Notch1 inhibitor: LY411575) are assessed by western blotting.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Oncoimmunology. 2018 Aug 23;7(11):e1461303.  [Abstract]

    The expression of Oct4 in A549 and H460 cells treated with or without rhIL-17A and these molecular inhibitors (NF-kB inhibitor: PDAC, Notch1 inhibitor: LY411575) are assessed by western blotting.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Oxid Med Cell Longev. 2017;2017:3869561.  [Abstract]

    N-formyl-Met-Leu-Phe (fMLP; 10 nM) are used to stimulate the cells for 2 min, and the polymorphonuclear neutrophils (PMNs) without fMLP serve as the control. NPS-2143 (100 nM), AMG 073 (100 nM), and PDTC (100 nM) are added and incubated. Protein expression results are representative of three experiments.

    查看 NF-κB 亚型特异性产品:

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    NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Pyrrolidinedithiocarbamate ammonium (Ammonium pyrrolidinedithiocarbamate) is a selective and blood-brain barrier (BBB) permeable NF-κB inhibitor.

    IC50 & Target[1]

    NF-κB

     

    体外研究
    (In Vitro)

    Pretreatment of cells with Pyrrolidinedithiocarbamate ammonium (Ammonium pyrrolidinedithiocarbamate; 3-1000 μM) dose-dependently attenuate IL-8 production[1].
    Furthermore, pyrrolidinedithiocarbamate ammonium (100 μM) suppresses the accumulation of IL-8 mRNA[1].
    Pyrrolidinedithiocarbamate ammonium inhibits the activation of NF-κB, because it suppresses both NF-κB DNA binding and NF-κB-dependent transcriptional activity. NF-κB inhibition with pyrrolidinedithiocarbamate ammonium decrease IL-8 production by intestinal epithelial cells[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Pyrrolidinedithiocarbamate ammonium 相关抗体:
    体内研究
    (In Vivo)

    The DSS+pyrrolidinedithiocarbamate ammonium-treated groupII exhibits suppression of shortening of intestinal length and reduction of DAI score. Activated NF-κB level and IL-1β and TNF-α levels are significantly lower in DSS+pyrrolidinedithiocarbamate ammonium-treated groupII. These findings suggest that suppression of NF-κB activity by pyrrolidinedithiocarbamate ammonium can delay the healing of mucosal tissue defects (erosions or ulcers) arising from inflammation, but that it can strongly suppress the expression of inf-lammatory cytokines (IL-1β and TNF-α), resulting in significant alleviation of colitis. pyrrolidinedithiocarbamate ammonium is useful for the treatment of ulcerative colitis[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    分子量

    164.29

    Formula

    C5H12N2S2
    CAS 号
    性状

    固体

    颜色

    White to yellow

    中文名称

    吡咯烷二硫代甲酸铵盐；四亚甲基二硫代氨基甲酸铵；1-吡咯烷二硫代羧酸铵盐；吡咯烷二硫代氨基甲酸铵；1-吡咯烷硫代羧酸铵盐；吡咯二硫代甲酸铵；四次甲基二硫代氨基甲酸铵；1-吡咯烷羰基二硫酸铵盐
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶解性数据
    In Vitro: 

    H2O 中的溶解度 : 50 mg/mL (304.34 mM; 超声助溶)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 6.0868 mL 30.4340 mL 60.8680 mL
    5 mM 1.2174 mL 6.0868 mL 12.1736 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    In Vivo:

    以下溶解方案，请直接配置工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 24 mg/mL (146.08 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 98.57%

    COA (267 KB) LCMS (92 KB)

    产品使用指南 (1538 KB)
    参考文献
    Cell Assay
    [1]

    The human colon cancer cell line HT-29 is obtained and cells are grown in modified McCoy’s 5A medium supplemented with 10% fetal bovine serum. To study the effect of pyrrolidinedithiocarbamate ammonium on IL-8 production, HT-29 cells in 96-well plates are induced with 20 ng/mL of IL-1β for 18 h. Various concentrations (3-1000 μM) of pyrrolidinedithiocarbamate or its vehicle (culture medium) are added to the cells 30 min prior to IL-1β stimulation. The concentration of IL-8 in the supernatant is determined using solid-phase enzyme-linked immunosorbent assay[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration

    Animal Administration: [2]Pyrrolidinedithiocarbamate is administered intraperitoneally to mice at dose levels of 100 and 50 mg/kg. Mice are divided into a DSS-untreated group (normal group), DSS-treated control group, DSS+pyrrolidinedithiocarbamate-treated groupI (low-dose group), and DSS+pyrrolidinedithiocarbamate-treated groupII (high-dose group). In each group, the disease activity index score (DAI score), intestinal length, histological score, and the levels of activated NF-κB and inflammatory cytokines (IL-1β and TNF-α) in tissue are measured[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    Pyrrolidinedithiocarbamate ammonium 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 6.0868 mL 30.4340 mL 60.8680 mL 152.1699 mL
    5 mM 1.2174 mL 6.0868 mL 12.1736 mL 30.4340 mL
    10 mM 0.6087 mL 3.0434 mL 6.0868 mL 15.2170 mL
    15 mM 0.4058 mL 2.0289 mL 4.0579 mL 10.1447 mL
    20 mM 0.3043 mL 1.5217 mL 3.0434 mL 7.6085 mL
    25 mM 0.2435 mL 1.2174 mL 2.4347 mL 6.0868 mL
    30 mM 0.2029 mL 1.0145 mL 2.0289 mL 5.0723 mL
    40 mM 0.1522 mL 0.7608 mL 1.5217 mL 3.8042 mL
    50 mM 0.1217 mL 0.6087 mL 1.2174 mL 3.0434 mL
    60 mM 0.1014 mL 0.5072 mL 1.0145 mL 2.5362 mL
    80 mM 0.0761 mL 0.3804 mL 0.7608 mL 1.9021 mL
    100 mM 0.0609 mL 0.3043 mL 0.6087 mL 1.5217 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Pyrrolidinedithiocarbamate5108-96-3Ammonium pyrrolidinedithiocarbamatePDTC APDC吡咯烷二硫代甲酸铵盐四亚甲基二硫代氨基甲酸铵1-吡咯烷二硫代羧酸铵盐吡咯烷二硫代氨基甲酸铵1-吡咯烷硫代羧酸铵盐吡咯二硫代甲酸铵四次甲基二硫代氨基甲酸铵1-吡咯烷羰基二硫酸铵盐NF-κBNuclear factor-κBNuclear factor-kappaBulcerativecolitisIL-1βTNF-αinflammationInhibitorinhibitorinhibit

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