1. Autophagy Apoptosis Metabolic Enzyme/Protease
  2. p62 Glutathione Peroxidase Ferroptosis
  3. RSL3

RSL3  (Synonyms: (1S,3R)-RSL3)

目录号: HY-100218A 纯度: 99.90%
COA 产品使用指南

RSL3 ((1S,3R)-RSL3) 是一种谷胱甘肽过氧化物酶 4 (GPX4) 的抑制剂 (ferroptosis 激动剂),可降低 GPX4 的表达,诱导头颈部癌细胞的肥大性死亡。在 HN3 耐受细胞中,可增加 p62 和 Nrf2 的蛋白水平,使 Keap1 灭活。

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RSL3 Chemical Structure

RSL3 Chemical Structure

CAS No. : 1219810-16-8

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥1455
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2 mg ¥950
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5 mg ¥1500
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10 mg ¥2500
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25 mg ¥5500
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50 mg ¥6600
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100 mg ¥8000
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Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 176 篇科研文献

WB
IHC
IF
Proliferation Assay

    RSL3 purchased from MCE. Usage Cited in: Cell Discov. 2022 May 3;8(1):40.  [Abstract]

    U2OS cells are treated with DMSO (Control) or OSMI-1 for 12 h, and then treated with RSL3 (10 μM) for different time as indicated. The levels of cellular ferrous iron are assessed by flow cytometry using FerroOrange.

    RSL3 purchased from MCE. Usage Cited in: Cell Discov. 2022 May 3;8(1):40.  [Abstract]

    U2OS cells are treated with DMSO (Control) or OSMI-1 for 12 h, and then treated with RSL3 (10 μM) for different time as indicated. Cells are then subjected to immunofluorescence microscopy with antibodies against TfR1 and GM130.

    RSL3 purchased from MCE. Usage Cited in: Cell Discov. 2022 May 3;8(1):40.  [Abstract]

    U2OS cells are treated with OSMI-1 (30 μM) or TMG (10 μM) for 12 h, and then induced with or without RSL3 (10 μM) for 6 h.

    RSL3 purchased from MCE. Usage Cited in: Nat Cancer. 2022 Apr;3(4):471-485.  [Abstract]

    Cellular responses of neuroblastoma cell lines to 72 h of RSL3 treatment: cells with MYCN amplification (black symbols), moderate MYCN expression (white circle) and lack thereof (white triangle).

    RSL3 purchased from MCE. Usage Cited in: Redox Biol. 2022 Jul 31;55:102408.  [Abstract]

    Immune blot detection of FSP1, GCLC, GCLM, and GPX4 in total protein extracts from the cell lines exposed to RSL3 or L-BSO for 24 h. The concentrations used are 0.25 and 1 μM RSL3 for CTV-1, Jurkat, K562 and HCT-116. For MOLT-16 RSL3 is used at 0.05 and 0.25 μM.

    RSL3 purchased from MCE. Usage Cited in: Int J Biol Sci. 2022 Jun 21;18(10):4135-4150.  [Abstract]

    CCK-8 assay assessed the cell viability of PANC1 and PaTU8988 cells treated DMSO, Erastin (4, 15 µM) and RSL3 (0.5, 5 µM) with or without SC-26196 (10 µM).

    RSL3 purchased from MCE. Usage Cited in: Cell Death Dis. 2022 Jul 20;13(7):630.  [Abstract]

    ROS levels are detected in pancreatic cancer cells from different treatment groups with a ROS Assay Kit. MDA and GSH-PX levels are detected in RSL3 (3 μM; 24 h) of treated Panc-1, MIA PaCa-2, and BxPC-3 cells.

    RSL3 purchased from MCE. Usage Cited in: Cell Death Dis. 2022 May 30;13(5):507.  [Abstract]

    HE staining of retinal tissue in mice that underwent sham or IR injury at 3 and 7 d after intravitreal injection of vehicle (DMSO) or 50 μM Erastin and 500 nM RSL-3.

    RSL3 purchased from MCE. Usage Cited in: Cell Death Dis. 2022 Sep 26;13(9):825.  [Abstract]

    MSCs are treated with Erastin (100 nmol/L) or RSL3 (10 nmol/L) or treated with Fer-1 (10 μmol/L) or Lip-1 (20 μmol/L) during SPIO labeling. Cells are harvested at 24 h. A Cell viability of MSCs under different treatment was measured by CCK-8 assay. Both of them reduce the cell viability of MSCs and induce the level of ROS and lipid peroxide in MSCs.

    RSL3 purchased from MCE. Usage Cited in: Cell Death Dis. 2022 Sep 26;13(9):825.  [Abstract]

    MSCs are treated with Erastin (100 nmol/L) or RSL3 (10 nmol/L) or treated with Fer-1 (10 μmol/L) or Lip-1 (20 μmol/L) during SPIO labeling. Cells are harvested at 24 h.

    RSL3 purchased from MCE. Usage Cited in: Redox Biol. 2021 Nov;47:102174.  [Abstract]

    HT1080 fibrosarcoma cells are exposed to RSL3 (10 μM, 24 h), and phorbol myristate acetate (PMA)-primed THP1 macrophage cells were exposed to crocidolite in the presence of absence of ferric ammonium citrate (Fe, 100 μg/ml) up to 96 h. Membrane-catch transmission electronmicroscopy (TEM) images of EVs on carbon grids.

    RSL3 purchased from MCE. Usage Cited in: Cell Death Dis. 2021 Nov 13;12(11):1079.  [Abstract]

    The protein levels of Keap1, Nrf2 and HO-1 in HCT116 and DLD-1 cells are measured by western blotting after treatment with RSL3 (1 μM), cetuximab (100 μg/ml) or their combination for 24 h.

    RSL3 purchased from MCE. Usage Cited in: Brain Res Bull. 2020 Oct;163:40-48.  [Abstract]

    Gpx4 and 4-HNE expression assessment by Western blotting after RSL3 treatment. Western blot analysis reveal that compared with the Mel group, the RSL3 group has significantly decreased and increased Gpx4 and 4-HNE expression, respectively.

    RSL3 purchased from MCE. Usage Cited in: Brain Res Bull. 2020 Oct;163:40-48.  [Abstract]

    Representative images and NeuN + cell quantification in the hippocampus via immunohistochemistry after RSL3 treatment. Compared with the Mel group, the RSL3 group has a significantly decreased NueN + cell number.
    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    RSL3 ((1S,3R)-RSL3) is an inhibitor of glutathione peroxidase 4 (GPX4) (ferroptosis activator), reduces the expression of GPX4 protein, and induces ferroptotic death of head and neck cancer cell. RSL3 increases the expression of p62 and Nrf2 and inactivates Keap1 in HN3-rslR cells[1].

    IC50 & Target

    Glutathione peroxidase 4[1]

    体外研究
    (In Vitro)

    RSL3 (0-8 μM,72 小时) 有效降低 HN3 细胞的活力,HN3 中的 IC50 为 0.48 μM,HN3-rslR 细胞中的 IC50 分别为 5.8 μM[1]
    RSL3 (0-8 μM,24 hours) 降低 HN3-rslR 细胞中 GPX4 蛋白的表达,增加 p62 和 Nrf2 的表达并使 Keap1 失活[1]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[1]

    Cell Line: HN3 cells, HN3-rslR cells
    Concentration: 0-8 μM
    Incubation Time: 72 hours
    Result: Showed IC50s of 0.48 µM in HN3 and 5.8 µM in HN3-rslR cells, respectively[1].

    Western Blot Analysis[1]

    Cell Line: HN3-rslR cells
    Concentration: 0-8 μM
    Incubation Time: 24 hours
    Result: Inhibited GPX4 expression, increased p62 and Nrf2 levels, and decreased Keap1 levels.
    体内研究
    (In Vivo)

    RSL3 (100 mg/kg,瘤内注射,每周两次,持续 20 天) 与葫芦巴碱 (HY-N0414) 联合使用可显著抑制携带 HN3R 细胞的小鼠的肿瘤生长[1]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Ten-week-old athymic BALB/c male nude mice (nu/nu) bearing HN3R cells[1]
    Dosage: 100 mg/kg in combination with trigonelline (50 mg/kg)
    Administration: Intratumorally twice per week for 20 days
    Result: Significantly reduced the volume of tumor combined with trigonelline in mice.
    分子量

    440.88

    Formula

    C23H21ClN2O5

    CAS 号
    性状

    固体

    颜色

    White to off-white

    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

    溶解性数据
    In Vitro: 

    DMSO 中的溶解度 : 100 mg/mL (226.82 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 2.2682 mL 11.3410 mL 22.6819 mL
    5 mM 0.4536 mL 2.2682 mL 4.5364 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)。-80°C储存时,请在2年内使用, -20°C储存时,请在1年内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    In Vivo:

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
    ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 5 mg/mL (11.34 mM); 悬浊液; 超声助溶

      此方案可获得 5 mg/mL的均匀悬浊液,悬浊液可用于口服和腹腔注射。

      1 mL 工作液为例,取 100 μL 50.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中,混合均匀。

      20% SBE-β-CD in Saline 的配制(4°C,储存一周):2 g SBE-β-CD(磺丁基醚 β-环糊精)粉末定容于 10 mL 的生理盐水中,完全溶解至澄清透明。
    • 方案 二

      请依序添加每种溶剂: 10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (5.67 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL(饱和度未知)的澄清溶液,此方案实验周期在半个月以上的动物实验酌情使用。

      1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

    以下溶解方案,请直接配置工作液。建议现用现配,在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比; 如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂: 50% PEG300    50% Saline

      Solubility: 20 mg/mL (45.36 mM); 悬浊液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    请输入您的动物体内配方组成:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    如果您的动物是免疫缺陷鼠或者体弱鼠,建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
    方案所需 助溶剂 包括:DMSO ,均可在 MCE 网站选购。 Tween 80,均可在 MCE 网站选购。
    计算结果
    工作液所需浓度 : mg/mL
    储备液配制方法 : mg 药物溶于 μL  DMSO(母液浓度为 mg/mL)。

    *In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

    您所需的储备液浓度超过该产品的实测溶解度,以下方案仅供参考,如有需要,请与 MCE 中国技术支持联系。
    动物实验体内工作液的配制方法 : 取 μL DMSO 储备液,加入 μL  μL ,混合均匀至澄清,再加 μL Tween 80,混合均匀至澄清,再加 μL 生理盐水
    连续给药周期超过半月以上,请谨慎选择该方案。
    请确保第一步储备液溶解至澄清状态,从左到右依次添加助溶剂。您可采用超声加热 (超声清洗仪,建议频次 20-40 kHz),涡旋吹打等方式辅助溶解。
    纯度 & 产品资料

    纯度: 99.90%

    参考文献

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)。-80°C储存时,请在2年内使用, -20°C储存时,请在1年内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.2682 mL 11.3410 mL 22.6819 mL 56.7048 mL
    5 mM 0.4536 mL 2.2682 mL 4.5364 mL 11.3410 mL
    10 mM 0.2268 mL 1.1341 mL 2.2682 mL 5.6705 mL
    15 mM 0.1512 mL 0.7561 mL 1.5121 mL 3.7803 mL
    20 mM 0.1134 mL 0.5670 mL 1.1341 mL 2.8352 mL
    25 mM 0.0907 mL 0.4536 mL 0.9073 mL 2.2682 mL
    30 mM 0.0756 mL 0.3780 mL 0.7561 mL 1.8902 mL
    40 mM 0.0567 mL 0.2835 mL 0.5670 mL 1.4176 mL
    50 mM 0.0454 mL 0.2268 mL 0.4536 mL 1.1341 mL
    60 mM 0.0378 mL 0.1890 mL 0.3780 mL 0.9451 mL
    80 mM 0.0284 mL 0.1418 mL 0.2835 mL 0.7088 mL
    100 mM 0.0227 mL 0.1134 mL 0.2268 mL 0.5670 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    产品名称:
    RSL3
    目录号:
    HY-100218A
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