1. Neuronal Signaling Stem Cell/Wnt Apoptosis
  2. Notch Apoptosis
  3. Tangeretin

Tangeretin  (Synonyms: 桔皮素; Tangeritin; NSC53909; NSC618905)

目录号: HY-N0133 纯度: 99.27%
COA 产品使用指南

Tangeretin (Tangeritin),黄酮类化合物,是 Notch-1 的有效抑制剂,具有抗炎,保护神经等作用。

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Tangeretin Chemical Structure

Tangeretin Chemical Structure

CAS No. : 481-53-8

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥605
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1 mg ¥250
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5 mg ¥550
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10 mg ¥900
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50 mg ¥2850
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100 mg ¥4250
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500 mg   询价  

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Top Publications Citing Use of Products
  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Tangeretin (Tangeritin), a flavonoid from citrus fruit peels, has been proven to play an important role in anti-inflammatory responses and neuroprotective effects in several disease models, and is a Notch-1 inhibitor.

IC50 & Target

Notch-1

体外研究
(In Vitro)

MTT 和集落形成测定表明,Tangeretin 增强了 GC 细胞的放射敏感性。Tangeretin 还减弱了辐射诱导的 EMT、GC 细胞的侵袭和迁移,同时降低了 Notch-1、Jagged1/2、Hey-1 和 Hes-1 的表达。Tangeretin 触发了 miR-410 的上调,miR-410 是一种肿瘤抑制性 microRNA。此外,miR-410 的重新表达可防止辐射诱导的 EMT 和细胞侵袭[1]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

在这项研究中,作者研究了 Tangeretin 在 3 周龄雄性 BALB/c 小鼠体内的抗 RSV 活性。斑块减少试验和荧光定量聚合酶链反应 (FQ-PCR) 表明,Tangeretin 可抑制 RSV 在小鼠肺部的复制[2]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

372.37

Formula

C20H20O7

CAS 号
性状

固体

颜色

Light yellow to yellow

中文名称

桔皮素;橙皮黄素;蜜桔黄酮;橘皮素

结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 1 year
-20°C 6 months
溶解性数据
In Vitro: 

DMSO 中的溶解度 : 25 mg/mL (67.14 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.6855 mL 13.4275 mL 26.8550 mL
5 mM 0.5371 mL 2.6855 mL 5.3710 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 1 year; -20°C, 6 months。-80°C储存时,请在1个月内使用, -20°C储存时,请在6个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

In Vivo:

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂: 10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (6.71 mM); 澄清溶液

    此方案可获得 ≥ 2.5 mg/mL(饱和度未知)的澄清溶液,此方案实验周期在半个月以上的动物实验酌情使用。

    1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
请输入您的动物体内配方组成:
%
DMSO +
+
%
Tween-80 +
%
Saline
如果您的动物是免疫缺陷鼠或者体弱鼠,建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
方案所需 助溶剂 包括:DMSO ,均可在 MCE 网站选购。 Tween 80,均可在 MCE 网站选购。
计算结果
工作液所需浓度 : mg/mL
储备液配制方法 : mg 药物溶于 μL  DMSO(母液浓度为 mg/mL)。
您所需的储备液浓度超过该产品的实测溶解度,以下方案仅供参考,如有需要,请与 MCE 中国技术支持联系。
动物实验体内工作液的配制方法 : 取 μL DMSO 储备液,加入 μL  μL ,混合均匀至澄清,再加 μL Tween 80,混合均匀至澄清,再加 μL 生理盐水
连续给药周期超过半月以上,请谨慎选择该方案。
请确保第一步储备液溶解至澄清状态,从左到右依次添加助溶剂。您可采用超声加热 (超声清洗仪,建议频次 20-40 kHz),涡旋吹打等方式辅助溶解。
纯度 & 产品资料

纯度: 99.27%

参考文献
Cell Assay

The effect of tangeretin on RAW264.7 cells was determined using a MTT assay as previously reported.(13) Briefly, RAW264.7 cells (1 × 104 cells/well) were seeded in a 96-well plate for 24 h and treated with different concentrations of tangeretin (6.3–50.0 μM) and dimethyl sulfoxide (DMSO) (vehicle control, 0.01 and 0.1%) for 10 or 48 h. The absorbance was measured at 570 nm using an enzyme immunoassay (EIA) reader (Thermo Scientific, Waltham, MA), and cell viability (%) was calculated as follows: [(absorbance of the test group – absorbance of the blank control)/(absorbance of the control group – absorbance of the blank control)] × 100.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration

Animal administration [2] The mice were maintained in an air-conditioned, pathogen-free room (temperature of 24 ± 2 °C, with a 12 h light/dark cycle from 6:00 am to 6:00 pm) with free access to food and water. Mice were randomly divided into five groups (n = 10) as follows: normal (control), RSV-challenged, and three treatment groups administered 25, 50, or 100 mg/kg/day tangeretin dissolved in saline. The control and RSV-challenged groups received equal volumes of saline. During the experiment, mice in the treatment groups were intragastrically administrated tangeretin for 3 days consecutively before RSV stimulation. Mice were lightly anesthetized with diethyl ether and intranasally challenged with RSV Long strain [6.7 × 106 plaque-forming units (PFU)] on day 4 after tangeretin treatment, while the control group was sham-infected with an equal volume of HEp-2 cell lysate, which was centrifuged under the same conditions as the viral suspensions. The mice were weighed during the experiment and sacrificed on day 5 post-infection after anesthetizing them with chloral hydrate (Figure 1B). The lung tissues were removed and weighed, and the lung index was calculated using the following formula: lung index = lung weight/body weight.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 1 year; -20°C, 6 months。-80°C储存时,请在1个月内使用, -20°C储存时,请在6个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6855 mL 13.4275 mL 26.8550 mL 67.1375 mL
5 mM 0.5371 mL 2.6855 mL 5.3710 mL 13.4275 mL
10 mM 0.2686 mL 1.3428 mL 2.6855 mL 6.7138 mL
15 mM 0.1790 mL 0.8952 mL 1.7903 mL 4.4758 mL
20 mM 0.1343 mL 0.6714 mL 1.3428 mL 3.3569 mL
25 mM 0.1074 mL 0.5371 mL 1.0742 mL 2.6855 mL
30 mM 0.0895 mL 0.4476 mL 0.8952 mL 2.2379 mL
40 mM 0.0671 mL 0.3357 mL 0.6714 mL 1.6784 mL
50 mM 0.0537 mL 0.2686 mL 0.5371 mL 1.3428 mL
60 mM 0.0448 mL 0.2238 mL 0.4476 mL 1.1190 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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目录号:
HY-N0133
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