Ustekinumab (Synonyms: Anti-Human IL-12/IL-23, Human Antibody)

目录号: HY-P9909 纯度: 98.42%: FAQs
Data Sheet SDS COA

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Ustekinumab 是一种抗 IL-12/IL-23 的人源单克隆 IgG1κ 抗体。

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Ustekinumab Chemical Structure

CAS No. : 815610-63-0

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MCE 顾客使用本产品发表的 1 篇科研文献

•Nat Immunol. 2021 Sep;22(9):1107-1117. [Abstract]

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生物活性
实验参考方法
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参考文献

生物活性

Ustekinumab is an anti-IL-12/IL-23 IgG1κ human monoclonal antibody.

IC₅₀ & Target

IL-12

IL-23

体外研究
(In Vitro)

Ustekinumab is a human monoclonal antibody that prevents human IL-12 and IL-23 from binding to the IL-12Rβ1 receptor chain of IL-12 (IL-12Rβ1/β2) and IL-23 (IL-12Rβ1/23R) receptor complexes on the surface of natural killer cells and T-cells^[1]. Ustekinumab is a human IgG1 kappa (κ) monoclonal antibody against the IL-12/IL-23 p40 subunit that prevents its interaction with IL-12Rβ1, thereby blocking subsequent signaling. The FcRn affinities at pH 6.0 fell in a narrow range for all 11 antibodies. The equilibrium dissociation constant (K_D) is calculated relative to Ustekinumab (Ustekinumab=1.0)^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

145625.97

CAS 号

815610-63-0

性状

液体

颜色

Colorless to light yellow

中文名称

尤特克单抗

运输条件

Shipping with dry ice.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料

纯度: 98.42%

选择批次：

Data Sheet (516 KB) SDS (251 KB)

COA (172 KB)

抗体抑制剂使用指南 (1152 KB)

参考文献

[1]. Montepaone M, et al. Profile of ustekinumab and its potential in the treatment of active psoriatic arthritis. Open Access Rheumatol. 2014 Feb 20;6:7-13. [Content Brief]

[2]. Schoch A, et al. Charge-mediated influence of the antibody variable domain on FcRn-dependent pharmacokinetics. Proc Natl Acad Sci U S A. 2015 May 12;112(19):5997-6002. [Content Brief]

Cell Assay ^[2]	HUVECs are grown on calibrated glass coverslips to a confluency of 80%. Subsequently, cells are incubated with 200 µg/mL Ustekinumab AlexaFluor488 and Briakinumab AlexaFluor594 for 20 min at 37°C, pH 7.3, washed three times, and chased in fresh medium for an additional 20 min before fixation. Antibodies are directly labeled. FcRn binding is quality controlled by SPR analysis. Cells are then counterstained with anti-FcRn monoclonal antibody DVN22, followed by anti-mouse secondary antibodies conjugated to AlexaFluor647. Imaging is performed on a Leica SP8 confocal microscope^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Montepaone M, et al. Profile of ustekinumab and its potential in the treatment of active psoriatic arthritis. Open Access Rheumatol. 2014 Feb 20;6:7-13. [Content Brief] [2]. Schoch A, et al. Charge-mediated influence of the antibody variable domain on FcRn-dependent pharmacokinetics. Proc Natl Acad Sci U S A. 2015 May 12;112(19):5997-6002. [Content Brief]

Cell Assay
^[2]

HUVECs are grown on calibrated glass coverslips to a confluency of 80%. Subsequently, cells are incubated with 200 µg/mL Ustekinumab AlexaFluor488 and Briakinumab AlexaFluor594 for 20 min at 37°C, pH 7.3, washed three times, and chased in fresh medium for an additional 20 min before fixation. Antibodies are directly labeled. FcRn binding is quality controlled by SPR analysis. Cells are then counterstained with anti-FcRn monoclonal antibody DVN22, followed by anti-mouse secondary antibodies conjugated to AlexaFluor647. Imaging is performed on a Leica SP8 confocal microscope^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Montepaone M, et al. Profile of ustekinumab and its potential in the treatment of active psoriatic arthritis. Open Access Rheumatol. 2014 Feb 20;6:7-13. [Content Brief]

[2]. Schoch A, et al. Charge-mediated influence of the antibody variable domain on FcRn-dependent pharmacokinetics. Proc Natl Acad Sci U S A. 2015 May 12;112(19):5997-6002. [Content Brief]

Ustekinumab 相关分类

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稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量		浓度		体积		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)	×	体积 _(start)	=	浓度 _(final)	×	体积 _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ustekinumab815610-63-0Anti-Human IL-12/IL-23, Human Antibody尤特克单抗Interleukin RelatedILInhibitorinhibitorinhibit

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Ustekinumab (Synonyms: Anti-Human IL-12/IL-23, Human Antibody)

Customer Review

MCE 顾客使用本产品发表的 1 篇科研文献

Ustekinumab 相关产品

•同靶点产品:

•同靶点蛋白产品:

生物活性

实验参考方法

纯度 & 产品资料

参考文献

Ustekinumab 相关分类

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稀释计算器

The molarity calculator equation

The dilution calculator equation

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Ustekinumab (Synonyms: Anti-Human IL-12/IL-23, Human Antibody)

Customer Review

Ustekinumab 相关抗体

MCE 顾客使用本产品发表的 1 篇科研文献

Ustekinumab 相关产品

•同靶点产品:

•同靶点蛋白产品:

生物活性

实验参考方法

纯度 & 产品资料

参考文献

Ustekinumab 相关抗体:

Ustekinumab 相关分类

摩尔计算器

稀释计算器

The molarity calculator equation

The dilution calculator equation

Keywords:

您最近查看的产品:

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