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  3. VIP(6-28)(human, rat, porcine, bovine)

VIP(6-28)(human, rat, porcine, bovine) 

目录号: HY-P1023 纯度: 98.85%
COA 产品使用指南

摩尔计算器

VIP(6-28)(human, rat, porcine, bovine) 是一种有效的外源性血管活性肠肽 (VIP) 拮抗剂。

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Custom Peptide Synthesis

VIP(6-28)(human, rat, porcine, bovine) Chemical Structure

VIP(6-28)(human, rat, porcine, bovine) Chemical Structure

CAS No. : 69698-54-0

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
500 μg ￥1400
In-stock
1 mg ￥1900
In-stock
5 mg ￥4800
In-stock
10 mg ￥7440
In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Customer Review

VIP(6-28)(human, rat, porcine, bovine) 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 1 篇科研文献

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

VIP(6-28)(human, rat, porcine, bovine) is an effective antagonist of the actions of exogenous vasoactive intestinal peptide (VIP) on cAMP.

IC50 & Target

VIP[1]
体外研究
(In Vitro)

VIP(6-28) is an effective VIP antagonist in the superior cervical ganglion (SCG) , and results obtained using this analog indicate that endogenous VIP can participate in a positive feedback loop in injured sympathetic neurons in which it enhances its own expression. VIP(6-28), when added to short-term cultures of adult SCG at a concentration of 10, 30, or 100 μM, reduces the increase in cAMP levels produced by stimulation with 10 μM VIP by 52, 64, or 81%, respectively. At any of these concentrations tested, VIP(6-28) by itself does not alter cAMP levels. In contrast to its ability to reduce the VIP-stimulated elevation in cAMP levels by 64%, the addition of 30 μM VIP(6-28) to culture medium does not significantly alter cAMP levels measured after stimulation of adult ganglia with either isoproterenol or forskolin (10 μM each). Similar results on the ability of VIP(6-28) to block VIP-stimulated increases in cAMP levels are obtained in neuron-enriched and in non-neuronal cell-enriched dissociated cultures[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

VIP(6-28)(human, rat, porcine, bovine) 相关抗体:
分子量

2816.28

Formula

C126H207N37O34S
CAS 号
性状

固体

颜色

White to off-white

Sequence

Phe-Thr-Asp-Asn-Tyr-Thr-Arg-Leu-Arg-Lys-Gln-Met-Ala-Val-Lys-Lys-Tyr-Leu-Asn-Ser-Ile-Leu-Asn-NH2
Sequence Shortening

FTDNYTRLRKQMAVKKYLNSILN-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
纯度 & 产品资料
参考文献
Kinase Assay
[1]

Adult rats are killed by decapitation. The SCGs are removed, desheathed, placed in organ culture, and maintained for 24 or 48 hr in F-12 defined medium equilibrated with 95% O2 and 5% CO2. Some ganglia are preincubated for 30 min in medium containing the VIP receptor antagonist VIP(6-28), and then transferred for 24 hr to medium containing both VIP(6-28) and an agonist. In experiments in which cAMP is to be measured, ganglia are removed from animals and preincubated for 30 min in F-12 medium containing 500 μM IBMX to prevent the metabolism of cAMP. Ganglia are then incubated for an additional 30 min in F-12 medium with IBMX and the compound to be studied. When the action of VIP(6-28) is examined, it is added to the medium during the last 5 min of the preincubation and throughout the incubation. Ascorbic acid (0.2 mg/mL) is added to cultures containing isoproterenol to retard oxidation of the catecholamine. No significant differences in peptide levels are detected between ganglia maintained in F-12 alone and those cultured in medium containing ascorbic acid[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

VIP(6-28)(human, rat, porcine, bovine)69698-54-0OthersInhibitorinhibitorinhibit

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产品名称：
VIP(6-28)(human, rat, porcine, bovine)
目录号：
HY-P1023
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