1. 诱导疾病模型产品 PROTAC Metabolic Enzyme/Protease Immunology/Inflammation Anti-infection
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  3. Cyclosporin A

Cyclosporin A  (Synonyms: 环孢素; Cyclosporine A; Ciclosporin A; CsA)

目录号: HY-B0579 纯度: 99.97%
COA 产品使用指南

Cyclosporin A (Cyclosporine A) 是一种免疫抑制剂,能与亲环素结合,抑制 protein phosphatase 2B (PP2B/calcineurin) 活性的IC50 值为 7 nM。Cyclosporin A 也抑制 CD11a/CD18 粘附分子。

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Cyclosporin A Chemical Structure

Cyclosporin A Chemical Structure

CAS No. : 59865-13-3

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥572
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100 mg ¥520
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200 mg ¥750
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500 mg ¥1380
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Other Forms of Cyclosporin A:

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Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 97 篇科研文献

WB
Proliferation Assay
IF

    Cyclosporin A purchased from MCE. Usage Cited in: Exp Mol Med. 2022 Feb;54(2):129-142.  [Abstract]

    Representative immunofluorescence images of STING with different degrees of polymerization in the Cyclosporine (CsA; 10 μM; for 24 h)- or DMSO-treated NP cells.

    Cyclosporin A purchased from MCE. Usage Cited in: Exp Mol Med. 2022 Feb;54(2):129-142.  [Abstract]

    Representative western blotting images of NLRP3, ASC, pro-CASP-1, cleaved CASP-1, GSDMD, and cleaved GSDMD in the (CsA; 10 μM; for 24 h)- or DMSO-treated NP cells.

    Cyclosporin A purchased from MCE. Usage Cited in: Exp Mol Med. 2022 Feb;54(2):129-142.  [Abstract]

    Representative western blotting images of cGAS and STING in the Cyclosporine (CsA; 10 μM; for 24 h)- or DMSO-treated NP cells.

    Cyclosporin A purchased from MCE. Usage Cited in: Redox Biol. 2022 Aug;54:102355.

    Cells are treated with or without Cyclosporine A (CsA; 2 μM) for 4 h in presence of NRC-03 (45 μg/mL). Cell viability determined by CCK-8.

    Cyclosporin A purchased from MCE. Usage Cited in: Redox Biol. 2022 Aug;54:102355.

    Cells are treated with or without Cyclosporine A (CsA; 2 μM) for 4 h in presence of NRC-03 (45 μg/mL). TUNEL immunofluorescence staining and quantification of TUNEL positive cells.

    Cyclosporin A purchased from MCE. Usage Cited in: Aging Dis. 2022 Oct 1;13(5):1546-1561.  [Abstract]

    Additionally, similar to P-gp siRNA silence, pharmacological inhibition of P-gp with Cyclosporine A (CsA; 22 mg/kg) significantly attenuates P-gp expression levels in ischemic brain as compared to vehicle treatment.

    Cyclosporin A purchased from MCE. Usage Cited in: Nat Commun. 2021 May 18;12(1):2915.  [Abstract]

    Immunoblot analysis of Cytochrome c from cytosolic extracts and mitochondria from indicated cells pre-treated with DMSO or Cyclosporine A (50 μM) for 1 h, followed with PFOS treatment (150 μM, 6 h).

    Cyclosporin A purchased from MCE. Usage Cited in: Cell Death Dis. 2021 Apr 20;12(5):413.  [Abstract]

    Inhibition of NR-induced increase of co-localized puncta (LC3 and TOM20) after 1 μMCyclosporin A (CsA) pretreatment.

    Cyclosporin A purchased from MCE. Usage Cited in: Cell Death Dis. 2021 Apr 1;12(4):336.  [Abstract]

    Immunofluorescence staining of subcellular localization of NFATC1 in RANK overexpression and control cells with or without treatment of 10 μg/mL Cyclosporin A (CsA).

    Cyclosporin A purchased from MCE. Usage Cited in: Cell Rep. 2021 Nov 30;37(9):110069.  [Abstract]

    Immunoblotting analysis of indicated apoptotic markers expression in CRC cells treated with Cyclosporine A (CsA; 2.5-5 μM) in combination with 5-FU or OXA.

    Cyclosporin A purchased from MCE. Usage Cited in: Cell Rep. 2021 Nov 30;37(9):110069.  [Abstract]

    Colony formation of CRC cells treated with Cyclosporine A (CsA; 2.5-5 μM) in combination with 5-FU or OXA for 48 h.

    Cyclosporin A purchased from MCE. Usage Cited in: Proc Natl Acad Sci U S A. 2021 Jan 12;118(2):e2009539118.  [Abstract]

    Calcineurin activities of the PCE after FK506 or Cyclosporine A (CsA; 4 µM) treatment Zebrafish.

    Cyclosporin A purchased from MCE. Usage Cited in: Br J Pharmacol. 2021 Jun;178(11):2305-2323.  [Abstract]

    Smooth muscle cells are treated cyclosporin A (CsA, 1 μM) or PDTC (10 μM) for 24 h before measuring the activity of PPARγ2 promoter.

    Cyclosporin A purchased from MCE. Usage Cited in: Cell Death Dis. 2018 May 22;9(6):598.  [Abstract]

    A549 cells are pretreated with 5 µM Cyclosporin A (CSA) for 2 h, followed by treatment with 80 μM Hirsutine for 24 h. equal amount of lysates are subjected to immunoprecipitation using anti-ANT1 antibody, the associated CypD is determined using immunoblotting.
    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Cyclosporin A (Cyclosporine A) is an immunosuppressant which binds to the cyclophilin and inhibits phosphatase activity of protein phosphatase 2B (PP2B/calcineurin) with an IC50 of 5 nM[3]. Cyclosporin A also inhibits CD11a/CD18 adhesion[8].

    IC50 & Target

    IC50: 7 nM (calcineurin)

    体外研究
    (In Vitro)

    Cyclosporin A 能够与 T 细胞中的亲环素结合[1]。Cyclosporin A 通过形成 Cyclophilin-Cyclosporin A 复合物来抑制神经钙蛋白[2]。Cyclosporin A 抑制受刺激细胞中的神经钙蛋白,IC50 值为 7 nM[3]。Cyclosporin A 抑制 NF-AT 的核转位[4]。Cyclosporin A 通过阻止 MTP 打开对线粒体产生影响,IC50 为 39 nM[5]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    体内研究
    (In Vivo)

    环孢菌素 A 具有免疫抑制活性,在小鼠、大鼠和豚鼠中通过胃肠外和口服给药均有活性[6]。环孢菌素A可用于器官移植预防排斥反应[7]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Clinical Trial
    分子量

    1202.61

    Formula

    C62H111N11O12

    CAS 号
    性状

    固体

    颜色

    White to off-white

    中文名称

    环孢霉素A;环孢素A;环孢多肽A;环孢灵;赛斯平;环孢素

    结构分类
    初始来源

    the fungus Beauveria nivea.

    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    溶解性数据
    In Vitro: 

    DMSO 中的溶解度 : 62.5 mg/mL (51.97 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

    Ethanol 中的溶解度 : 50 mg/mL (41.58 mM; 超声助溶)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 0.8315 mL 4.1576 mL 8.3152 mL
    5 mM 0.1663 mL 0.8315 mL 1.6630 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    In Vivo:

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
    ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂: 5% DMSO    40% PEG300    5% Tween-80    50% Saline

      Solubility: 2.62 mg/mL (2.18 mM); 悬浊液; 超声助溶

    • 方案 二

      请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.08 mg/mL (1.73 mM); 悬浊液; 超声助溶

      此方案可获得 2.08 mg/mL的均匀悬浊液,悬浊液可用于口服和腹腔注射。

      1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;再向上述体系中加入 50 μL Tween-80,混合均匀;然后再继续加入 450 μL 生理盐水 定容至 1 mL

      生理盐水的配制:将 0.9 g 氯化钠,溶解于 ddH₂O 并定容至 100 mL,可以得到澄清透明的生理盐水溶液。

    以下溶解方案,请直接配置工作液。建议现用现配,在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比; 如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂: Corn Oil

      Solubility: 20 mg/mL (16.63 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    请输入您的动物体内配方组成:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    如果您的动物是免疫缺陷鼠或者体弱鼠,建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
    方案所需 助溶剂 包括:DMSO ,均可在 MCE 网站选购。 Tween 80,均可在 MCE 网站选购。
    计算结果
    工作液所需浓度 : mg/mL
    储备液配制方法 : mg 药物溶于 μL  DMSO(母液浓度为 mg/mL)。

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    您所需的储备液浓度超过该产品的实测溶解度,以下方案仅供参考,如有需要,请与 MCE 中国技术支持联系。
    动物实验体内工作液的配制方法 : 取 μL DMSO 储备液,加入 μL  μL ,混合均匀至澄清,再加 μL Tween 80,混合均匀至澄清,再加 μL 生理盐水
    连续给药周期超过半月以上,请谨慎选择该方案。
    请确保第一步储备液溶解至澄清状态,从左到右依次添加助溶剂。您可采用超声加热 (超声清洗仪,建议频次 20-40 kHz),涡旋吹打等方式辅助溶解。
    纯度 & 产品资料

    纯度: 99.97%

    参考文献
    Cell Assay
    [3]

    Immunosuppressive agents are dissolved in ethanol at concentrations 1000-fold more than the concentration desired for cell treatments. Cells (106) are suspended in 1 mL of complete medium in microcentrifuge tubes; 1 μL of ethanol or of the ethanolic solution of Cyclosporin A is added, and the cells are incubated at 37°C for 1 hr. Cells are washed twice with 1 mL of PBS on ice and lysed in 50 μL of hypotonic buffer containing 50 mM Tris (pH 7.5); 0.1 mM EGTA; 1 mM EDTA; 0.5 mM dithiothreitol; and 50 μg of phenylmethylsulfonyl fluoride, 50 μg of soybean trypsin inhibitor, 5 μg of leupeptin, and 5 μg of Kiker 52G per mL. Lysates are subjected to three cycles of freezing in liquid nitrogen followed by thawing at 30°C and then are centrifuged at 4°C for 10 min at 12,000×g.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [6]

    Rats are immunized on day 0 i.p. with 0 5 mL and mice i.v. with 0 2 mL of a 10% suspension of washed sheep erythrocytes (SE). To elicit a secondary response, mice are boosted 8-11 weeks after the primary immunization with an i.v. injection of 0-2 mL of 0 25% washed SE (107 cells). For prolonged treatment the animals receive on the average 45 mg/kg cyclosporin A daily in the food during 13 weeks. These rats are immunized 5 days before killing.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    参考文献

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    Ethanol / DMSO 1 mM 0.8315 mL 4.1576 mL 8.3152 mL 20.7881 mL
    5 mM 0.1663 mL 0.8315 mL 1.6630 mL 4.1576 mL
    10 mM 0.0832 mL 0.4158 mL 0.8315 mL 2.0788 mL
    15 mM 0.0554 mL 0.2772 mL 0.5543 mL 1.3859 mL
    20 mM 0.0416 mL 0.2079 mL 0.4158 mL 1.0394 mL
    25 mM 0.0333 mL 0.1663 mL 0.3326 mL 0.8315 mL
    30 mM 0.0277 mL 0.1386 mL 0.2772 mL 0.6929 mL
    40 mM 0.0208 mL 0.1039 mL 0.2079 mL 0.5197 mL
    DMSO 50 mM 0.0166 mL 0.0832 mL 0.1663 mL 0.4158 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    产品名称:
    Cyclosporin A
    目录号:
    HY-B0579
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