1. 染料试剂

Dye Reagents

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.

Dye Reagents (168):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-15534
    JC-1 3520-43-2
    JC-1 是一种荧光亲脂性羰花青染料,可以用来衡量线粒体的膜电位。
    JC-1
  • HY-D0711
    Cardiogreen 3599-32-4
    Cardiogreen 是一种荧光剂,广泛应用于医疗诊断领域,比如测定心输出量、肝脏功能和肝血流量,以及眼科血管造影术等。
    Cardiogreen
  • HY-12591A
    D-Luciferin 2591-17-5 98.11%
    D-luciferin 是荧光素酶的天然底物,可催化生物发光昆虫产生典型的黄绿光。
    D-Luciferin
  • HY-15558
    Hoechst 33258 23491-44-3 98.17%
    Hoechst 33258 是一种荧光染料,用于 DNA 染色。
    Hoechst 33258
  • HY-D0913
    1-Methyl-7-nitroisatoic anhydride 73043-80-8 >98.0%
    1-methyl-7-nitroisatoic anhydride是一种检测局部核苷酸的试剂。
    1-Methyl-7-nitroisatoic anhydride
  • HY-D0718
    Nile Red 7385-67-3 98.06%
    Nile Red 是一种重要染料,通过荧光显微法和流式细胞光度术来测定细胞内的脂肪滴。
    Nile Red
  • HY-66019
    FITC 3326-32-7
    FITC 是 fluorescein 的衍生物,应用广泛,包括流式细胞技术。
    FITC
  • HY-15559A
    Hoechst 33342 trihydrochloride 875756-97-1 99.79%
    Hoechst 33342 trihydrochloride 结合到 DNA 小沟,用于细胞 DNA 荧光染色。
    Hoechst 33342 trihydrochloride
  • HY-D0079
    Dihydroethidium 104821-25-2 98.09%
    Dihydroethidium(Hydroethidine; PD-MY 003)是过氧化物指示剂,在细胞质中具有蓝色荧光,直到被氧化。
    Dihydroethidium
  • HY-15623
    Hoechst 33258 analog 258843-62-8 99.16%
    Hoechst 33258类似物是DNA蓝色荧光染料。
    Hoechst 33258 analog
  • HY-15937
    5(6)-FAM SE 117548-22-8 98.98%
    5(6)-FAM SE是一色胺反应的绿色荧光染料,广泛应用于蛋白质标记。
    5(6)-FAM SE
  • HY-15559
    Hoechst 33342 23491-52-3 98.75%
    Hoechst染料是DNA蓝色荧光染料,Hoechst 33342是其中一种。
    Hoechst 33342
  • HY-14536
    Methylene Blue 61-73-4 98.22%
    Methylene blue(Basic Blue 9)是一种色素内镜检查染料,可喷涂于胃肠道粘膜。
    Methylene Blue
  • HY-B1046
    Clofazimine 2030-63-9 98.09%
    Clofazimine是一种脂溶性亚氨基酚嗪类染料, 具有显著的抗炎效果, 与其它抗分支杆菌药物联用来AIDS和克罗恩病。
    Clofazimine
  • HY-15941
    5(6)-FITC 27072-45-3
    5(6)-FITC(FITC)是一种胺活性衍生物的荧光染料,具有广泛的应用,作为抗体和其他探针标记,用荧光显微镜,流式细胞仪、免疫荧光方法如ELISA和Western印迹实验。
    5(6)-FITC
  • HY-15922
    Luminol 521-31-3 99.29%
    Luminol(Diogenes reagent)是化学发光试剂,与一定的氧化剂反应时能发出蓝光。
    Luminol
  • HY-19948
    Leucomethylene blue Mesylate 1236208-20-0 >98.0%
    Leucomethylene blue甲磺酸盐是Methylene blue(亚甲基蓝)的一种还原形式, 亚甲基蓝是一种化学指示剂和生物染色剂。
    Leucomethylene blue Mesylate
  • HY-15925
    NBT 298-83-9 99.93%
    NBT(Nitro BT;p-Nitrotetrazolium blue)是脱氢酶底物,与碱性磷酸酶底物BCIP一起用于western blotting和免疫组化染色。
    NBT
  • HY-D0723
    5(6)-TAMRA SE 246256-50-8
    5(6)-TAMRA SE是一荧光染料,结合寡聚核苷酸和用于DNA测序。
    5(6)-TAMRA SE
  • HY-D0802
    Biotin NHS 35013-72-0
    生物素NHS是一种氨基活性生物素试剂和染料。
    Biotin NHS