1. 染料试剂

Dye Reagents

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.

Dye Reagents (237):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-D0940
    H2DCFDA 4091-99-0 99.14%
    H2DCFDA 是一种可渗透细胞的非荧光 (但含荧光素) 探针。
    H2DCFDA
  • HY-15534
    JC-1 3520-43-2 99.46%
    JC-1 是一种荧光亲脂性羰花青染料,可以用来衡量线粒体的膜电位。
    JC-1
  • HY-12591A
    D-Luciferin 2591-17-5 98.81%
    D-luciferin 是荧光素酶的天然底物,可催化生物发光昆虫产生典型的黄绿光。
    D-Luciferin
  • HY-D0913
    1-Methyl-7-nitroisatoic anhydride 73043-80-8 >98.0%
    1-methyl-7-nitroisatoic anhydride是一种检测局部核苷酸的试剂。
    1-Methyl-7-nitroisatoic anhydride
  • HY-D0718
    Nile Red 7385-67-3
    Nile Red 是一种重要染料,通过荧光显微法和流式细胞光度术来测定细胞内的脂肪滴。
    Nile Red
  • HY-D0711
    Cardiogreen 3599-32-4 >98.0%
    Cardiogreen 是一种荧光剂,广泛应用于医疗诊断领域,比如测定心输出量、肝脏功能和肝血流量,以及眼科血管造影术等。
    Cardiogreen
  • HY-D0814
    DAPI dihydrochloride 28718-90-3 >98.0%
    DAPI dihydrochloride (4',6-diamidino-2-phenylindole) 是一种与富含A-T序列的DNA结合的荧光染料。
    DAPI dihydrochloride
  • HY-D0938
    CFSE 150347-59-4
    CFSE 是可以跟踪细胞分裂的荧光染料。
    CFSE
  • HY-15558
    Hoechst 33258 23491-44-3 99.13%
    Hoechst 33258 是一种荧光染料,用于 DNA 染色。
    Hoechst 33258
  • HY-15559A
    Hoechst 33342 trihydrochloride 875756-97-1 99.79%
    Hoechst 33342 trihydrochloride 结合到 DNA 小沟,用于细胞 DNA 荧光染色。
    Hoechst 33342 trihydrochloride
  • HY-66019
    FITC 3326-32-7
    FITC 是 fluorescein 的衍生物,应用广泛,包括流式细胞技术。
    FITC
  • HY-D0815
    Propidium Iodide 25535-16-4 >98.0%
    Propidium iodide是可用于细胞染色的红色荧光染料。
    Propidium Iodide
  • HY-D0802
    Biotin NHS 35013-72-0
    生物素NHS是一种氨基活性生物素试剂和染料。
    Biotin NHS
  • HY-D0079
    Dihydroethidium 104821-25-2 99.77%
    Dihydroethidium(Hydroethidine; PD-MY 003)是过氧化物指示剂,在细胞质中具有蓝色荧光,直到被氧化。
    Dihydroethidium
  • HY-12591B
    D-Luciferin potassium salt 115144-35-9 99.20%
    D-Luciferin potassium salt 是荧光素酶的底物,可催化生物发光昆虫产生典型的黄绿光。
    D-Luciferin potassium salt
  • HY-15928
    PNPP 4264-83-9 99.85%
    PNPG(4-Nitrophenyl-β-D- glucopyranoside)是显色的β-D-葡糖苷酶底物,裂解后会产生黄色溶液。
    PNPP
  • HY-B1046
    Clofazimine 2030-63-9 98.78%
    Clofazimine是一种脂溶性亚氨基酚嗪类染料, 具有显著的抗炎效果, 与其它抗分支杆菌药物联用来AIDS和克罗恩病。
    Clofazimine
  • HY-111382
    Diphenylterazine 344940-63-2
    Diphenylterazine 是一种生物发光剂。
    Diphenylterazine
  • HY-D0218
    Thioflavin T 2390-54-7 >98.0%
    Thioflavin T 是一种阳离子 Benzothiazole 染料,在组织切片中与淀粉样蛋白结合后荧光强度增强。
    Thioflavin T
  • HY-D0041
    Calcein-AM 148504-34-1
    Calcein-AM是用于测定细胞活力的可以渗透细胞的荧光染料。
    Calcein-AM