1. 染料试剂

Dye Reagents

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.

Dye Reagents (237):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-D0940
    H2DCFDA 4091-99-0 99.74%
    H2DCFDA 是一种可渗透细胞的非荧光 (但含荧光素) 探针。
    H2DCFDA
  • HY-15534
    JC-1 3520-43-2 99.46%
    JC-1 是一种荧光亲脂性羰花青染料,可以用来衡量线粒体的膜电位。
    JC-1
  • HY-12591A
    D-Luciferin 2591-17-5 98.81%
    D-luciferin 是荧光素酶的天然底物,可催化生物发光昆虫产生典型的黄绿光。
    D-Luciferin
  • HY-D0718
    Nile Red 7385-67-3
    Nile Red 是一种重要染料,通过荧光显微法和流式细胞光度术来测定细胞内的脂肪滴。
    Nile Red
  • HY-D0814
    DAPI dihydrochloride 28718-90-3 >98.0%
    DAPI dihydrochloride (4',6-diamidino-2-phenylindole) 是一种与富含A-T序列的DNA结合的荧光染料。
    DAPI dihydrochloride
  • HY-D0913
    1-Methyl-7-nitroisatoic anhydride 73043-80-8 >98.0%
    1-methyl-7-nitroisatoic anhydride是一种检测局部核苷酸的试剂。
    1-Methyl-7-nitroisatoic anhydride
  • HY-D0938
    CFSE 150347-59-4
    CFSE 是可以跟踪细胞分裂的荧光染料。
    CFSE
  • HY-D0711
    Cardiogreen 3599-32-4 >98.0%
    Cardiogreen是一种低毒性荧光剂,广泛用于医学诊断,如确定心输出量,肝功能和肝血流量,以及用于眼科血管造影。
    Cardiogreen
  • HY-15559A
    Hoechst 33342 trihydrochloride 875756-97-1 99.79%
    Hoechst 33342 trihydrochloride 结合到 DNA 小沟,用于细胞 DNA 荧光染色。
    Hoechst 33342 trihydrochloride
  • HY-66019
    FITC 3326-32-7
    FITC是荧光素的衍生物,用于标记胺。
    FITC
  • HY-15558
    Hoechst 33258 23491-44-3 99.13%
    Hoechst 33258是一种荧光染料,当与 dsDNA 结合时会发出蓝色荧光。
    Hoechst 33258
  • HY-D0815
    Propidium Iodide 25535-16-4 >98.0%
    Propidium iodide是可用于细胞染色的红色荧光染料。
    Propidium Iodide
  • HY-D0079
    Dihydroethidium 104821-25-2 99.77%
    Dihydroethidium(Hydroethidine; PD-MY 003)是过氧化物指示剂,在细胞质中具有蓝色荧光,直到被氧化。
    Dihydroethidium
  • HY-D0802
    Biotin NHS 35013-72-0
    Biotin NHS是用于制备生物素化表面或多肽的氨基反应性生物素试剂。
    Biotin NHS
  • HY-D0041
    Calcein-AM 148504-34-1
    Calcein-AM是用于测定细胞活力的可以渗透细胞的荧光染料。
    Calcein-AM
  • HY-12591B
    D-Luciferin potassium salt 115144-35-9 99.20%
    D-Luciferin potassium salt 是荧光素酶的底物,可催化生物发光昆虫产生典型的黄绿光。
    D-Luciferin potassium salt
  • HY-D0083
    DiI 41085-99-8 >98.0%
    DiI(DiIC18(3))是亲脂性膜染料,侧向扩散染色整个细胞。 DiI被激发后发射橙红色荧光。 荧光在水中很微弱,但是结合到细胞膜中时荧光信号强且具有光稳定性。
    DiI
  • HY-111382
    Diphenylterazine 344940-63-2
    Diphenylterazine 是一种生物发光剂。
    Diphenylterazine
  • HY-D0090
    MQAE 162558-52-3 99.75%
    MQAE 是一种荧光氯离子指示剂,它比 36Cl 同位素示踪法和微电极法更灵敏,更有选择性。
    MQAE
  • HY-B1046
    Clofazimine 2030-63-9 98.78%
    Clofazimine是一种脂溶性亚氨基酚嗪类染料, 具有显著的抗炎效果, 与其它抗分支杆菌药物联用来AIDS和克罗恩病。
    Clofazimine