1. Anti-infection Cell Cycle/DNA Damage Stem Cell/Wnt Autophagy Metabolic Enzyme/Protease
  2. SARS-CoV Casein Kinase Autophagy 11β-HSD
  3. Emodin

Emodin  (Synonyms: 大黄素; Frangula emodin)

目录号: HY-14393 纯度: 99.20%
COA 产品使用指南

Emodin (Frangula emodin) 是一种蒽醌衍生物,是一种抗 COVID-19 化合物。Emodin 阻断 SARS 病毒刺突蛋白和血管紧张素转化酶 2 (ACE2) 的相互作用。Emodin 抑制酪蛋白激酶 2 (CK2)。 具有抗炎和抗癌作用。Emodin 还是一种有效的选择性 11β-HSD1 抑制剂,对人和小鼠 11β-HSD1 的 IC50 分别为 186 和 86 nM。Emodin 可改善饮食诱导的肥胖小鼠的代谢紊乱。

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Emodin Chemical Structure

Emodin Chemical Structure

CAS No. : 518-82-1

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥220
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50 mg ¥200
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100 mg ¥320
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200 mg ¥512
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Top Publications Citing Use of Products

    Emodin purchased from MCE. Usage Cited in: Am J Transl Res. 2020 May 15;12(5):1851-1861.  [Abstract]

    Emodin reverses 5-Fu chemoresistance in CRC via downregulation of PI3K/Akt signaling pathway. SW480/5-Fu cells are treated with 5-Fu or/and Emodin for 72 h. Then, the protein expressions of Bax, Bcl-2, cleaved caspase3, ERK, Akt, p-ERK and p-Akt in SW480/5-Fu cells are investigated by Western-blot.

    Emodin purchased from MCE. Usage Cited in: Am J Transl Res. 2020 May 15;12(5):1851-1861.  [Abstract]

    Emodin resensitizes SW480/5-Fu cells to 5-Fu. SW480/5-Fu cells are treated with 5-Fu or/and Emodin for 72 h. The proliferation of SW480/5-Fu cell is detected by Ki67 immunofluorescence.

    查看 Casein Kinase 亚型特异性产品:

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Emodin (Frangula emodin), an anthraquinone derivative, is an anti-SARS-CoV compound. Emodin blocks the SARS coronavirus spike protein and angiotensin-converting enzyme 2 (ACE2) interaction[1]. Emodin inhibits casein kinase-2 (CK2). Anti-inflammatory and anticancer effects[2]. Emodin is a potent selective 11β-HSD1 inhibitor with the IC50 of 186 and 86 nM for human and mouse 11β-HSD1, respectively. Emodin ameliorates metabolic disorder in diet-induced obese mice[3].

    IC50 & Target[1][2][3]

    SARS-CoV

     

    CK2α Wild-type

    1.4 μM (IC50, at ATP concentration is 10 μM)

    CK2α Wild-type

    5.9 μM (IC50, at ATP concentration is 50 μM)

    mouse 11β-HSD1

    86 nM (IC50)

    human 11β-HSD1

    186 nM (IC50)

    体外研究
    (In Vitro)

    Emodin (10-400 μM) 以剂量依赖性方式阻断 S 蛋白与 ACE2 的结合,IC50 值为 200 μM[1]
    Emodin (5-50 μM) 以剂量依赖的方式抑制 S 蛋白假型逆转录病毒的感染性。Emodin 阻断 SARS-CoV S 蛋白与 Vero E6 细胞的结合[1]
    Emodin 抑制酪蛋白激酶 2 (CK2),IC50 为 5.9 CK2α 野生型、Ile174Ala 突变体和 His160Ala 突变体在 ATP 浓度下分别为 50 μM、30.0 和 7.1 μM。CK2α 野生型的 IC50 分别为 1.40 和 38.00 μM,Val66Ala 突变体在 ATP 浓度下为 10 μM[2]
    Emodin 表现出低抑制性对小鼠和人类 2 型 11β-羟基类固醇脱氢酶 (11β-HSD2) 的活性,IC50 高于 1 mM,表明 Emodin 对人类和小鼠 11β-的选择性超过 5000 倍HSD1 酶超过 2 型同工酶[3]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[1]

    Cell Line: Vero E6 cells transfected with the plasmid encoding ACE2
    Concentration: 0, 5, 25, 50 μM
    Incubation Time: 24 hours
    Result: Vero cells treated with 50 μM remained 82.4±3.8% viability, the anti-SARS-CoV activity was not due to toxicity.
    体内研究
    (In Vivo)

    Emodin (单次口服100或200 mg/kg) 抑制正常C57BL/6J雄性小鼠11β-HSD1活性[3]
    Emodin (100 mg/kg;口服;bid) 改善饮食诱导的肥胖 (DIO) 小鼠的胰岛素敏感性和脂质代谢,降低血糖和肝脏 PEPCK 以及葡萄糖-6-磷酸酶 mRNA[3]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: C57BL/6J male mice[3]
    Dosage: 100 or 200 mg/kg
    Administration: Acute administered p.o. ; Two hours later, the mice were killed by cervical dislocation,
    Result: Significantly inhibited liver 11β-HSD1 enzymatic activity by 17.6 and 31.3% and mesenteric fat 11β-HSD1 enzymatic activity by 21.5 and 46.7% at 100 or 200 mg/kg, respectively.
    Animal Model: DIO mice (C57BL/6J male mice were fed a formulated research diet)[3]
    Dosage: 100 mg/kg
    Administration: Oral gavage; twice per day; for 35 days
    Result: Reduced fasting glucose concentrations to 77.2% of the vehicle control mice after 7 days of treatment, and these remained significantly lower throughout the treatment period.
    Exhibited a significant reduction in blood glucose levels at all time-points following oral glucose challenge after 24 days of treatment.
    Evoked a significantly greater reduction in blood glucose values 40 and 90 min after insulin injection after 28 days of treatment.
    The serum insulin level was also significantly reduced, to 66.2% of control mice, after 35 days of treatment.
    Improved the lipid profiles. The serum triglyceride and total cholesterol levels were significantly reduced by 19.3 and 12.5% after 35 days of treatment, respectively.
    Caused a 22.7% reduction of non-esterified free fatty acid (NEFA) level.
    Lowered body weight and appetite from day 18 of the treatment; their body weights were reduced by 13.9% at the end of treatment.
    Clinical Trial
    分子量

    270.24

    Formula

    C15H10O5

    CAS 号
    性状

    固体

    颜色

    Yellow to orange

    中文名称

    大黄素

    结构分类
    初始来源
    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    溶解性数据
    In Vitro: 

    Acetone 中的溶解度 : 10.87 mg/mL (40.22 mM; 超声助溶)

    DMSO 中的溶解度 : 5.41 mg/mL (20.02 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

    Ethanol 中的溶解度 : < 1 mg/mL (insoluble)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 3.7004 mL 18.5021 mL 37.0041 mL
    5 mM 0.7401 mL 3.7004 mL 7.4008 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 1 year; -20°C, 6 months。-80°C储存时,请在1个月内使用, -20°C储存时,请在6个月内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    In Vivo:

    以下溶解方案,请直接配置工作液。建议现用现配,在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比; 如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂: 0.5% CMC-Na/saline water

      Solubility: 3.33 mg/mL (12.32 mM); Suspened solution; 超声助溶

    • 方案 二

      请依序添加每种溶剂: 0.5% MC  0.5% Tween-80

      Solubility: 10 mg/mL (37.00 mM); 悬浊液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    纯度 & 产品资料

    纯度: 99.20%

    参考文献

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 1 year; -20°C, 6 months。-80°C储存时,请在1个月内使用, -20°C储存时,请在6个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO / Acetone 1 mM 3.7004 mL 18.5021 mL 37.0041 mL 92.5104 mL
    5 mM 0.7401 mL 3.7004 mL 7.4008 mL 18.5021 mL
    10 mM 0.3700 mL 1.8502 mL 3.7004 mL 9.2510 mL
    15 mM 0.2467 mL 1.2335 mL 2.4669 mL 6.1674 mL
    20 mM 0.1850 mL 0.9251 mL 1.8502 mL 4.6255 mL
    Acetone 25 mM 0.1480 mL 0.7401 mL 1.4802 mL 3.7004 mL
    30 mM 0.1233 mL 0.6167 mL 1.2335 mL 3.0837 mL
    40 mM 0.0925 mL 0.4626 mL 0.9251 mL 2.3128 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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