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  3. L-Ascorbic acid

L-Ascorbic acid  (Synonyms: L-抗坏血酸; 维生素C; L-Ascorbate; Vitamin C)

目录号: HY-B0166 纯度: 99.92%
COA 产品使用指南

L-Ascorbic acid (L-Ascorbate),一种电子供体,是一种内源性抗氧化剂。L-Ascorbic acid 选择性抑制 Cav3.2 通道 (Cav3.2 channels),IC50 为 6.5 μM。L-Ascorbic acid 还是一种胶原沉积促进剂和弹性生成抑制剂。L-Ascorbic acid通过产生活性氧 (ROS) 和选择性损伤癌细胞表现出抗癌作用。

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L-Ascorbic acid Chemical Structure

L-Ascorbic acid Chemical Structure

CAS No. : 50-81-7

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MCE 顾客使用本产品发表的 36 篇科研文献

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

L-Ascorbic acid (L-Ascorbate), an electron donor, is an endogenous antioxidant agent. L-Ascorbic acid inhibits selectively Cav3.2 channels with an IC50 of 6.5 μM. L-Ascorbic acid is also a collagen deposition enhancer and an elastogenesis inhibitor[1][2][3]. L-Ascorbic acid exhibits anti-cancer effects through the generation of reactive oxygen species (ROS) and selective damage to cancer cells[4].

IC50 & Target

T-type calcium channel

 

Microbial Metabolite

 

Human Endogenous Metabolite

 

体外研究
(In Vitro)

L-Ascorbic acid 的抗癌作用由钠依赖性维生素 C 转运蛋白 2 (SVCT-2) 决定,它是 L-Ascorbic acid 的转运蛋白。L-Ascorbic acid (0.1 μM-2 mM) 根据 SVCT-2 表达和 L-ascorbic acid 摄取表现出抗癌作用。人结直肠癌细胞系对 L-Ascorbic acid 表现出不同的反应,这主要取决于 SVCT-2 的表达水平[4]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[4]

Cell Line: High SVCT-2 expressing cell lines Sw620, Sw480, LoVo, SNU-C4; Low SVCT-2 expressing cell lines HCT15, HCT116, DLD-1, CoLo-205
Concentration: 0, 0.1 μM, 1 μM, 10 μM, 0.1 mM, 0.5 mM, 1 mM, and 2 mM
Incubation Time: 24 hours
Result: Some high SVCT-2 expressing cancer cells demonstrated a dramatic cell-autonomous inhibitory effect of L-ascorbic acid.
Low SVCT-2 expressing cell lines showed biphasic responses to L-ascorbic acid.

Western Blot Analysis[4]

Cell Line: Sw620, Sw480, LoVo, SNU-C4, HCT15, HCT116, DLD-1, CoLo-205 cell lines
Concentration: 1 mM
Incubation Time:
Result: The cell lines showed different levels of SVCT-2 expression in western blot analyses: Sw620, Sw480, and Lovo expressed high levels of SVCT-2 whereas HCT116, HCT15, and DLD-1 expressed low levels.
体内研究
(In Vivo)

L-Ascorbic acid/Tolbutamide 在正常 (60 mg/kg) 和糖尿病 (40 mg/kg) 条件下以剂量依赖性方式产生降血糖活性。在 L-Ascorbic acid 存在的情况下,与 Tolbutamide 匹配对照相比,Tolbuatmide (20 mg/kg) 起效早且维持时间更长[5]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Normal rats:Albino rats of either sex weighing between 125-175 g[5]
Dosage: Group I received L-ascorbic acid 60 mg/kg, Group II received Tolbutamide 20 mg/kg and Group III was given L-ascorbic acid (60 mg/kg) prior to the administration of tolbutamide 20 mg/kg
Administration: Administered orally
Result: L-ascorbic acid at the dose of 60 mg/kg produced 50.91% blood glucose reduction at 0.5 h and 20 mg/kg body weight of Tolbutamide produced 33% at 4 h as peak effects. In the presence of L-ascorbic acid (60 mg/kg), the action of Tolbutamide was early in onset and maintained for 6 h.
Animal Model: Diabetic rats:Albino rats of either sex weighing between 125 to 175 g were fasted overnight before injection with Alloxan[5]
Dosage: Group I received L-ascorbic acid 40 mg/kg and Group II received Tolbutamide 20 mg/kg while Group III was given L-ascorbic acid 40 mg/kg prior to Tolbutamide administration (20 mg/kg).
Administration: Oral administration
Result: L-ascorbic acid (40 mg/kg alone) produced 42.53% blood glucose reduction at 1.5 h and Tolbutamide 20 mg/kg produced 45.09 at 4 h. Administration of L-ascorbic acid 40 mg/kg body weight prior to Tolbutamide produced antidiabetic activity at 0.5 h and was maintained for 6 h.
Clinical Trial
分子量

176.12

Formula

C6H8O6

CAS 号
性状

固体

颜色

White to off-white

中文名称

L-抗坏血酸;维生素C;维生素 C

结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO 中的溶解度 : 100 mg/mL (567.79 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

H2O 中的溶解度 : ≥ 100 mg/mL (567.79 mM)

* "≥" means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 5.6779 mL 28.3897 mL 56.7795 mL
5 mM 1.1356 mL 5.6779 mL 11.3559 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

In Vivo:

以下溶解方案,请直接配置工作液。建议现用现配,在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比; 如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶。

  • 方案 一

    请依序添加每种溶剂: PBS

    Solubility: 100 mg/mL (567.79 mM); 澄清溶液; 超声助溶 (<60°C)

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳,请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度,如有需要,请与 MCE 中国技术支持联系。
纯度 & 产品资料

纯度: 99.92%

参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 5.6779 mL 28.3897 mL 56.7795 mL 141.9487 mL
5 mM 1.1356 mL 5.6779 mL 11.3559 mL 28.3897 mL
10 mM 0.5678 mL 2.8390 mL 5.6779 mL 14.1949 mL
15 mM 0.3785 mL 1.8926 mL 3.7853 mL 9.4632 mL
20 mM 0.2839 mL 1.4195 mL 2.8390 mL 7.0974 mL
25 mM 0.2271 mL 1.1356 mL 2.2712 mL 5.6779 mL
30 mM 0.1893 mL 0.9463 mL 1.8926 mL 4.7316 mL
40 mM 0.1419 mL 0.7097 mL 1.4195 mL 3.5487 mL
50 mM 0.1136 mL 0.5678 mL 1.1356 mL 2.8390 mL
60 mM 0.0946 mL 0.4732 mL 0.9463 mL 2.3658 mL
80 mM 0.0710 mL 0.3549 mL 0.7097 mL 1.7744 mL
100 mM 0.0568 mL 0.2839 mL 0.5678 mL 1.4195 mL

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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