1. GPCR/G Protein Neuronal Signaling Autophagy Apoptosis
  2. PINK1/Parkin Opioid Receptor Autophagy Mitophagy Ferroptosis Apoptosis
  3. Matrine

Matrine  (Synonyms: 苦参碱; Matridin-15-one; Vegard; α-Matrine)

目录号: HY-N0164 纯度: ≥98.0%
COA 产品使用指南

Matrine (Matridin-15-one) 是一种在槐属植物中发现的生物碱,可作为 kappa 阿片受体和 u-受体激动剂。Matrine 具有多种药理作用,包括抗癌、抗氧化应激、抗炎和抗凋亡作用,可用于人类非小细胞肺癌、肝癌、甲状腺乳头状癌和急性肾损伤 (AKI) 等疾病的研究。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

Matrine Chemical Structure

Matrine Chemical Structure

CAS No. : 519-02-8

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥1074
In-stock
100 mg ¥976
In-stock
200 mg ¥1674
In-stock
500 mg ¥2976
In-stock
1 g   询价  
5 g   询价  

* Please select Quantity before adding items.

Customer Review

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products

    Matrine purchased from MCE. Usage Cited in: Biomed Pharmacother. 2020 Aug;128:110327.  [Abstract]

    Overexpression of miR-182-5p abolishes the Matrine-induced increase in caspase3 expression and decrease in Bcl-2 expression in vivo. Tumor sections are subjected to IHC staining using an antibody specific for caspase3 and Bcl-2.

    Matrine purchased from MCE. Usage Cited in: Exp Ther Med. 2019 May;17(5):3775-3780.  [Abstract]

    Matrine increases the expression of pro‑apoptotic proteins in RC‑4B/C cells. RC‑4B/C cells are treated with 0.1, 0.5 and 2.5 mg/ml of matrine for 24 h and the expression of Bim, Bax and Bcl‑2 is determined by western blotting analysis. RC‑4B/C cells treated with 0.1% DMSO are used as negative controls. β‑actin is used as internal reference gene in pituitary cancer cells.

    Matrine purchased from MCE. Usage Cited in: Exp Ther Med. 2019 May;17(5):3775-3780.  [Abstract]

    Matrine promotes the nuclear Foxo3a localization and inhibits the levels of cytoplasmic Foxo3a. RC‑4B/C cells are treated with increasing concentrations of matrine for 24 h. The levels of Foxo3 are determined by western blotting analysis. β‑tubulin is used as cytoplasmic marker and lamin B1 is used as nuclear marker in RC‑4B/C cells. The gray values of Foxo3a is shown in histogram in different concentrations of matrine‑treated pituitary cancer cells.
    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Matrine (Matridin-15-one) is an alkaloid found in plants from the Sophora genus that can act as a kappa opioid receptor and u-receptor agonist. Matrine has a variety of pharmacological effects, including anti-cancer, anti-oxidative stress, anti-inflammation and anti-apoptosis effects. Matrine is potential in the research of disease like human non-small cell lung cancer, hepatoma, papillary thyroid cancer and acute kidney injury (AKI)[1][2][3][4][5].

    IC50 & Target

    κ Opioid Receptor/KOR

     

    μ Opioid Receptor/MOR

     

    体外研究
    (In Vitro)

    Matrine (0-1.5 mg/mL,24-72 h) 抑制 A549 和 SMMC-7721 细胞的生长[1]
    Matrine (25 μg/mL,6 h) 抑制 A549 细胞的迁移[1]
    Matrine (0-1 mg/mL,48 h) 通过降低 A549 和 SMMC-7721 细胞的 Bcl-2/Bax 蛋白比率诱导细胞凋亡[1]
    Matrine (0-1 mg/mL,48 h) 抑制miR-182-5p表达并诱导PTC细胞凋亡[2]
    Matrine (10 μM,48 小时) 通过降低 ROS 水平和促炎细胞因子 (包括 IL-1β、IL-6 和 TNF-α) 抑制顺铂诱导的 HK2 细胞氧化损伤和炎症[4]
    Matrine (10 μM,48 h) 通过激活 SIRT3/OPA1 通路逆转顺铂诱导的 HK2 细胞的线粒体功能[4]
    Matrine (0 -20 nM,12 h) 通过抑制线粒体自噬和 PINK1/Parkin 通路促进 HepG2 细胞凋亡[5]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[1]

    Cell Line: A549, SMMC-7721 cells
    Concentration: 0-500 μg/mL for A549 cells, 0-1.5 mg/mL for SMMC-7721 cells
    Incubation Time: 24-72 h
    Result: Inhibited the growth of A549 and SMMC-7721 cells.

    Western Blot Analysis[1]

    Cell Line: A549, SMMC-7721 cells
    Concentration: 100-250 μg/mL for A549 cells, 0.5-1 mg/mL for SMMC-7721 cells
    Incubation Time: 24 h
    Result: Down-regulated the expression of anti-apoptotic protein (Bcl-2) and up-regulated the level of pro-apoptotic protein (bax).

    Immunofluorescence[4]

    Cell Line: HK2 cells
    Concentration: 10 μM
    Incubation Time: 48 h
    Result: Increased SIRT3 expression reduced under cisplatin stimuli.
    体内研究
    (In Vivo)

    Matrine (灌胃,40 和 80 mg/kg,连续 16 天,异种移植雄性C57BL/6小鼠模型) 抑制肿瘤生长和转移而不影响体重[3]
    腹膜内注射,每天 5 mg/kg,连续四天) 减轻顺铂诱导的 AKI 小鼠的肾损伤和细胞凋亡,以及减少炎症反应和激活 SIRT3/OPA1 轴并挽救肾线粒体功能障碍[4]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Xenograft male C57BL/6mice model (LLC cells)[3]
    Dosage: 40 and 80 mg/kg for 16 consecutive days
    Administration: Intragastric administration
    Result: Inhibited tumors growth.
    Decreased the ratio of CD206+/F4/80+, promoted the expression of CD4+ and CD8+ T cells, and inhibited the expression of Th2 in tumor and spleen tissues.
    Animal Model: Cisplatin-induced acute kidney injury (AKI) mice model[4]
    Dosage: 5 mg/kg daily for 4 days
    Administration: Intraperitoneal injections
    Result: Attenuated tubular injury observed in AKI mice, including renal tubular necrosis,formation of tubular casts, cytoplasmic vacuoles and renal infiltrationof inflammatory cells in mice.
    Decreased serum levels of TNF-a and IL-6 and the phosphorylation of NF-κB, activated SIRT3/OPA1 axis and improved mitochondrial function.
    分子量

    248.36

    Formula

    C15H24N2O

    CAS 号
    性状

    固体

    颜色

    White to off-white

    中文名称

    苦参碱;母菊碱

    结构分类
    初始来源
    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    溶解性数据
    In Vitro: 

    DMSO 中的溶解度 : ≥ 50 mg/mL (201.32 mM; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

    H2O 中的溶解度 : 20 mg/mL (80.53 mM; 超声助溶)

    * "≥" means soluble, but saturation unknown.

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 4.0264 mL 20.1321 mL 40.2641 mL
    5 mM 0.8053 mL 4.0264 mL 8.0528 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    * 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    In Vivo:

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
    ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (10.07 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL(饱和度未知)的澄清溶液。

      1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;再向上述体系中加入 50 μL Tween-80,混合均匀;然后再继续加入 450 μL 生理盐水 定容至 1 mL

      生理盐水的配制:将 0.9 g 氯化钠,溶解于 ddH₂O 并定容至 100 mL,可以得到澄清透明的生理盐水溶液。
    • 方案 二

      请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (10.07 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL(饱和度未知)的澄清溶液。

      1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中,混合均匀。

      20% SBE-β-CD in Saline 的配制(4°C,储存一周):2 g SBE-β-CD(磺丁基醚 β-环糊精)粉末定容于 10 mL 的生理盐水中,完全溶解至澄清透明。

    以下溶解方案,请直接配置工作液。建议现用现配,在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比; 如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂: PBS

      Solubility: 37.5 mg/mL (150.99 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳,请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度,如有需要,请与 MCE 中国技术支持联系。
    纯度 & 产品资料
    参考文献

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 4.0264 mL 20.1321 mL 40.2641 mL 100.6603 mL
    5 mM 0.8053 mL 4.0264 mL 8.0528 mL 20.1321 mL
    10 mM 0.4026 mL 2.0132 mL 4.0264 mL 10.0660 mL
    15 mM 0.2684 mL 1.3421 mL 2.6843 mL 6.7107 mL
    20 mM 0.2013 mL 1.0066 mL 2.0132 mL 5.0330 mL
    25 mM 0.1611 mL 0.8053 mL 1.6106 mL 4.0264 mL
    30 mM 0.1342 mL 0.6711 mL 1.3421 mL 3.3553 mL
    40 mM 0.1007 mL 0.5033 mL 1.0066 mL 2.5165 mL
    50 mM 0.0805 mL 0.4026 mL 0.8053 mL 2.0132 mL
    60 mM 0.0671 mL 0.3355 mL 0.6711 mL 1.6777 mL
    80 mM 0.0503 mL 0.2517 mL 0.5033 mL 1.2583 mL
    DMSO 100 mM 0.0403 mL 0.2013 mL 0.4026 mL 1.0066 mL

    * 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    您最近查看的产品:

    Your information is safe with us. * Required Fields.

       产品名称:

     

    * 需求量:

    * 客户姓名:

     

    * Email:

    * 电话:

     

    * 公司或机构名称:

       留言给我们:

    Bulk Inquiry

    Inquiry Information

    产品名称:
    Matrine
    目录号:
    HY-N0164
    需求量: