1. Metabolic Enzyme/Protease
  2. Glycosidase
  3. PNGase F (Lyophilized)

PNGase F (Lyophilized)  (Synonyms: 糖基肽酶F (冻干))

目录号: HY-P2929C
产品使用指南 技术支持

PNGase F (Lyophilized) 是一种由重组大肠杆菌表达的糖苷酶。PNGase F (Lyophilized) 可以选择性水解蛋白质中所有与天冬酰胺酶 (Asn) 连接的 N-糖,但含有 α1-3 岩藻糖苷键的除外。其裂解位点是天冬酰胺酶 (Asn) 和最内层 GlcNAc 之间的糖苷键,从而将与天冬酰胺连接的糖分子从糖蛋白中释放出来,并将天冬酰胺转化为天冬氨酸。裂解的糖型包括高甘露糖、混合糖和复合寡糖。

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PNGase F (Lyophilized) Chemical Structure

PNGase F (Lyophilized) Chemical Structure

CAS No. : 83534-39-8

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PNGase F (Lyophilized) 的其他形式现货产品:

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MCE 顾客使用本产品发表的 1 篇科研文献

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

PNGase F (Lyophilized) is a glycosidase expressed by recombinant E. coli. PNGase F (Lyophilized) can selectively hydrolyze all N-glycans linked to Asn in proteins except those containing α1-3 fucosidic bonds. The cleavage site is the glycosidic bond between Asn and the innermost GIcNAc, which releases sugar molecules linked to asparagine from glycoproteins and converts asparagine to aspartic acid. The cleaved glycoforms include high mannose, hybrid and complex oligosaccharides[1].

CAS 号
中文名称

糖基肽酶F (冻干)

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献
  • 摩尔计算器

  • 稀释计算器

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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PNGase F (Lyophilized)
目录号:
HY-P2929C
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