2. Membrane Transporter/Ion Channel Apoptosis
  3. Sodium Channel Apoptosis
  4. Psalmotoxin 1

Psalmotoxin 1  (Synonyms: PcTx1; Psalmopoeus cambridgei toxin-1)

目录号: HY-P1411 纯度: ≥99.0%
COA 产品使用指南

摩尔计算器

Psalmotoxin 1 (PcTx1) 是一种蛋白质毒素，可与酸敏感离子通道 1a (ASIC1a) 的亚基-亚基界面结合。Psalmotoxin 1 通过增加 ASIC1a 对 H+ 的表观亲和力 (apparent affinity) 来有效地、选择性抑制 ASIC1a (IC50: 0.9 nM)。Psalmotoxin 1 可诱导细胞凋亡 (apoptosis)，抑制癌细胞迁移、增殖和侵袭。Psalmotoxin 1 可用于癌症或神经系统疾病的研究。

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Custom Peptide Synthesis

Psalmotoxin 1 Chemical Structure

Psalmotoxin 1 Chemical Structure

CAS No. : 880107-52-8

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规格 价格 是否有货 数量
100 μg ￥7000
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Psalmotoxin 1 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 1 篇科研文献

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Psalmotoxin 1 (PcTx1) is a protein toxin that can bind at subunit-subunit interfaces of acid-sensing ion channel 1a (ASIC1a). Psalmotoxin 1 is a potent and slective ASIC1a inhibitor (IC50: 0.9 nM) by increasing the apparent affinity for H+ of ASIC1a. Psalmotoxin 1 can induce cell apoptosis, also inhibits cell migration, proferliration and invasion of cancer cells. Psalmotoxin 1 can be used in the research of cancers, or neurological disease[1][3][4][6].

IC50 & Target

IC50: 0.9 nM (ASIC1a), 50 nM (ASIC1b, ASIC2a, and ASIC3)[6].
体外研究
(In Vitro)

Psalmotoxin 1 (20 nM，125 s) 通过显著改变稳态脱敏曲线以降低 H+ 浓度来抑制 ASIC1a 电流[1]。
Psalmotoxin 1 (30 nM) 与 Ca2+ 竞争结合 ASIC1a 通道[1]。
Psalmotoxin 1 (100 或 200 ng，24-72 小时) 显著减弱 MCF-7 和 MDA-MB-231 细胞的迁移、增殖和侵袭[4]。
Psalmotoxin 1 (100 ng/mL，24 h) 显著抑制酸诱导的增加参与细胞内钙和 LDH 释放，诱导髓核细胞 (NPC) 细胞凋亡和细胞周期停滞[5]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Psalmotoxin 1 相关抗体:

Cell Proliferation Assay[4]

Cell Line: MCF-7 and MDA-MB-231 cells
Concentration: 100 or 200 ng
Incubation Time: 24, 48, 72 h
Result: Inhibited the cell migration, proliferation and invasion of breast cancer cells.

Western Blot Analysis[5]

Cell Line: Nucleus pulposus cells (NPCs)
Concentration: 100 ng/mL
Incubation Time: 24 h
Result: Decreased Bax and cleaved caspase-3 expression, and increased Bcl-2 expression.
体内研究
(In Vivo)

Psalmotoxin 1 (icv，1 ng/kg，单次剂量) 通过直接抑制 ASIC1a 在有意识的中风模型中发挥神经保护作用[2]。
Psalmotoxin 1 (尾静脉注射，10 ng/kg，每日一次，持续 7 天) 抑制乳腺癌小鼠模型的肿瘤生长[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male spontaneously hypertensive rats (SHR)[2]
Dosage: 1 ng/kg, a single dose.
Administration: Intracerebroventricular (i.c.v.) injection
Result: Reduced cortical and striatal infarct volumes measured 72 h post-stroke.
Reduced the severity of motor deficit at 1 and 3 days after stroke compared to control rats.
Displayed an anti-apoptotic effect in the occluded hemisphere (reduced stroke-induced caspase-3 positive cells).
Animal Model: Female nude BALB/C mice (orthotopic implantation, MCF-7 and MDA-MB-231 cells)[3]
Dosage: 10 ng/kg, daily for 7 days.
Administration: Tail vein injection
Result: Inhibited breast tumor growth.
分子量

4689.39

Formula

C200H312N62O57S6
CAS 号
性状

固体

颜色

White to off-white

Sequence

Glu-Asp-Cys-Ile-Pro-Lys-Trp-Lys-Gly-Cys-Val-Asn-Arg-His-Gly-Asp-Cys-Cys-Glu-Gly-Leu-Glu-Cys-Trp-Lys-Arg-Arg-Arg-Ser-Phe-Glu-Val-Cys-Val-Pro-Lys-Thr-Pro-Lys-Thr (Disulfide bridge: Cys3-Cys18, Cys10-Cys23, Cys17-Cys33)
Sequence Shortening

EDCIPKWKGCVNRHGDCCEGLECWKRRRSFEVCVPKTPKT (Disulfide bridge: Cys3-Cys18, Cys10-Cys23, Cys17-Cys33)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
纯度 & 产品资料

纯度: ≥99.0%

COA (256 KB)

产品使用指南 (1538 KB)
参考文献

Psalmotoxin 1 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Psalmotoxin 1880107-52-8PcTx1 Psalmopoeus cambridgei toxin-1Psalmotoxin1Psalmotoxin-1PcTx 1PcTx-1Psalmopoeus cambridgei toxin1Psalmopoeus cambridgei toxin 1Psalmopoeus cambridgei toxin-1Sodium ChannelApoptosisNa channelsNa+ channelsSpider toxinhypertensiveneuroprotectionbreast cancerMDA-MB-231MCF-7NPCsapoptosisInhibitorinhibitorinhibit

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