1. 重组蛋白
  2. Immune Checkpoint Proteins CD Antigens
  3. Inhibitory Checkpoint Molecules Stimulatory Immune Checkpoint Molecules T Cell CD Proteins B Cell CD Proteins Macrophage CD Proteins Monocyte CD Proteins Dendritic Cell CD Proteins
  4. B7-1/CD80
  5. B7-1/CD80 蛋白, Human (HEK293, mFc)

B7-1/CD80 蛋白, Human (HEK293, mFc)

目录号: HY-P704260
产品使用指南 技术支持

CD80 蛋白在 T 淋巴细胞活化中发挥关键作用,通过 CD28 结合促进增殖和细胞因子产生。相反,与 CTLA-4 的相互作用会抑制 T 细胞活化。B7-1/CD80 蛋白, Human (HEK293, mFc) 是重组的 B7-1/CD80 蛋白,由 HEK293 表达,带有 C-mFc 标签。

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     可免费申领三个不同产品的试用装。

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描述

The CD80 protein plays a key role in T lymphocyte activation, promoting proliferation and cytokine production through CD28 binding. In contrast, interaction with CTLA-4 inhibits T cell activation. B7-1/CD80 Protein, Human (HEK293, mFc) is the recombinant human-derived B7-1/CD80 protein, expressed by HEK293, with C-mFc labeled tag.

研究背景

CD80 蛋白在 T 淋巴细胞激活所必需的共刺激信号中发挥着关键作用,与 CD28 结合后可促进 T 细胞增殖和细胞因子产生。相反,当与 CTLA-4 相互作用时,CD80 会引发相反的作用,抑制 T 细胞活化。在微生物感染的情况下,CD80 还充当腺病毒 B 亚型的受体,进一步证明了它在免疫反应中的多方面参与及其对不同信号线索的识别。CD80 对 T 细胞激活的复杂调节强调了其在协调适应性免疫反应中的重要性,并强调了其在不同生理环境中的多功能功能。

种属

Human

表达系统

HEK293

标签

C-mFc

蛋白编号

P33681-1 (V35-N242)

基因 ID

941

蛋白结构
N-term
B7-1 (V35-N242)
Accession # P33681
mFc
C-term
蛋白长度

Extracellular Domain

同用名
rHuB7-1/CD80, Fc Chimera; BB1; CD28LG; CD28LG1; LAB7
预测分子量
50.9 kDa
分子量

Approximately 65-85 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

糖基化修饰
Yes
构象/形态
Monomer
纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
B7-1/CD80 Protein, Human (HEK293, mFc)
目录号:
HY-P704260
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